Skip to main content
SpringerLink
Log in

Enhanced Lycopene Content in Blakeslea trispora by Effective Mutation-Screening Method

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The zygomycete fungus Blakeslea trispora is usually used as a natural source of lycopene and β-carotene. In this study, the B. trispora (−) strain, a major mating type for lycopene production, was treated with N+ ion implantation and N-methyl-N′-nitro-N-nitrosoguanidine (NTG), and further isolated on the screening plates supplemented with lovastatin and crude extracts of trisporic acid (CTA). After several rounds of screening, four mutants with higher yield of lycopene and biomass were isolated. Among these mutants, I5 obtained with N+ ion implantation showed a maximum lycopene yield (28.8 mg/g), which was 64 % higher than the parent strain (17.5 mg/g) in the production of lycopene. The results indicated that N+ ion implantation is more suitable for B. trispora (−) than NTG treatment, and the addition of lovastatin promoted the generation of positive mutant and CTA amplified the color differences between colonies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Kong, K.-W., Khoo, H.-E., Prasad, K. N., Ismail, A., Tan, C.-P., & Rajab, N. F. (2010). Molecules, 15, 959–987.

    Article  CAS  Google Scholar 

  2. Rao, A. V., & Agarwal, S. (2000). Journal of the American College of Nutrition, 19, 563–569.

    Article  CAS  Google Scholar 

  3. Erdman, J. W., Ford, N. A., & Lindshield, B. L. (2009). Archives of Biochemistry and Biophysics, 483, 229–235.

    Article  CAS  Google Scholar 

  4. Swarthout, E. J. (1963). U.S. Pat. No. 3,097,146, Publication date: 09-07-1963

  5. Cerdá-Olmedo, E. (1989). In E. J. Vandamme (Ed.), Biotechnology of vitamins, pigments and growth factors (pp. 27–42). Amsterdam, The Netherlands: Elsevier Applied Science.

    Chapter  Google Scholar 

  6. Alper, H., Miyaoku, K., & Stephanopoulos, G. (2006). Applied Microbiology and Biotechnology, 72, 968–974.

    Article  CAS  Google Scholar 

  7. Papaioannou, E. H., & Liakopoulou-Kyriakides, M. (2010). Food and Bioproducts Processing, 88, 305–311.

    Article  CAS  Google Scholar 

  8. Das, A., Yoon, S. H., Lee, S. H., Kim, J. Y., Oh, D. K., & Kim, S. W. (2007). Applied Microbiology and Biotechnology, 77, 505–512.

    Article  CAS  Google Scholar 

  9. Wang, G. (2002). Metabolic Engineering, 4, 193–201.

    Article  CAS  Google Scholar 

  10. Shimada, H., Kondo, K., Fraser, P. D., Miura, Y., Saito, T., & Misawa, N. (1998). Applied and Environmental Microbiology, 64, 2676–2680.

    CAS  Google Scholar 

  11. Alberts, A., Chen, J., Kuron, G., Hunt, V., Huff, J., Hoffman, C., et al. (1980). Proceedings of the National Academy of Sciences, 77, 3957–3961.

    Article  CAS  Google Scholar 

  12. Thomas, D., Harris, R., Kirk, J., & Goodwin, T. (1967). Phytochemistry, 6, 361–366.

    Article  CAS  Google Scholar 

  13. Schmidt, A. D., Heinekamp, T., Matuschek, M., Liebmann, B., Bollschweiler, C., & Brakhage, A. A. (2005). Applied Microbiology and Biotechnology, 67, 549–555.

    Article  CAS  Google Scholar 

  14. Sun, J., Li, H., & Yuan, Q. (2012). PLoS One, 7, e46110.

    Article  CAS  Google Scholar 

  15. Rodriguez, M., Teresa, A., De, E., Antonio, C., Costa Perez, J., Ruiz, O., Antonio, M., Fraile Yecora, N., De La, F. M. J. L., Rodriguez Saiz, M. (2009). EP Patent 1,471,151, Publication date: 27-10-2004

  16. Shi, Y. Q., Xin, X. L., & Yuan, Q. P. (2012). Biotechnology Letters, 34, 849–852.

    Article  CAS  Google Scholar 

  17. Schachtschabel, D., Menzel, K. D., Krauter, G., David, A., Roth, M., Horn, U., et al. (2010). Applied Microbiology and Biotechnology, 88, 241–249.

    Article  CAS  Google Scholar 

  18. Schimek, C., Kleppe, K., Saleem, A.-R., Voigt, K., Burmester, A., & Wöstemeyer, J. (2003). Mycological Research, 107, 736–747.

    Article  CAS  Google Scholar 

  19. Gu, S. B., Li, S. C., Feng, H. Y., Wu, Y., & Yu, Z. L. (2008). Applied Microbiology and Biotechnology, 78, 201–209.

    Article  CAS  Google Scholar 

  20. Mehta, B. J., Obraztsova, I. N., & Cerda-Olmedo, E. (2003). Applied and Environmental Microbiology, 69, 4043–4048.

    Article  CAS  Google Scholar 

  21. Sandesh Kamath, B., Vidhyavathi, R., Sarada, R., & Ravishankar, G. A. (2008). Bioresource Technology, 99, 8667–8673.

    Article  CAS  Google Scholar 

  22. Mehta, B., & Cerdá-Olmedo, E. (1995). Applied Microbiology and Biotechnology, 42, 836–838.

    Article  CAS  Google Scholar 

  23. Navarro, E., Sandmann, G., & Torres-Martinez, S. (1995). Experimental mycology, 19, 186–190.

    Article  CAS  Google Scholar 

  24. Ohnishi, J., Mizoguchi, H., Takeno, S., & Ikeda, M. (2008). Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 649, 239–244.

    Article  CAS  Google Scholar 

  25. Su, C., Zhou, W., Fan, Y., Wang, L., Zhao, S., & Yu, Z. (2006). Journal of Industrial Microbiology and Biotechnology, 33, 1037–1042.

    Article  CAS  Google Scholar 

  26. Gu, S. B., Yao, J. M., Yuan, Q. P., Xue, P. J., Zheng, Z. M., Wang, L., et al. (2006). Journal of Industrial Microbiology and Biotechnology, 72, 456–461.

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 21176105), the Natural Science Foundation of Jiangsu Province, China (Grant No. BK20130130), the Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (Grant No. KLIB-KF201105) and the Innovation and entrepreneurship training program for college students in Jiangsu Province (Grant No. 201310295030X).

Author information

Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Qiang Wang, Wei Luo, Qiu-ya Gu, Ling-ran Feng, Han-guang Li & Xiao-bin Yu

Authors
  1. Qiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qiu-ya Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ling-ran Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Han-guang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiao-bin Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao-bin Yu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Q., Luo, W., Gu, Qy. et al. Enhanced Lycopene Content in Blakeslea trispora by Effective Mutation-Screening Method. Appl Biochem Biotechnol 171, 1692–1700 (2013). https://doi.org/10.1007/s12010-013-0468-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0468-8

Keywords

Search

Navigation