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Bioconversion of Sodium Dodecyl Sulphate to Rhamnolipid by Pseudomonas aeruginosa: A Novel and Cost-Effective Production Strategy

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Abstract

The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Pseudomonas aeruginosa S15 capable of concomitant SDS degradation and biosurfactant synthesis. SDS-based rhamnolipid production was proved on SDS minimal agar plates using cetyl trimethylammonium bromide–methylene blue method and optimised in SDS-based minimal salt (SBS) medium. SDS proved to be an ideal carbon source for rhamnolipid synthesis with a high substrate to product conversion rate yielding 6.9 g/l of rhamnolipids from 1 g/l SDS in 5 days. Fast atom bombardment mass spectroscopy analysis of the purified biosurfactant proved the presence of mono- and di-rhamnolipids, viz., Rha-C10-C10, Rha-C10-C12 and Rha-Rha-C10-C10 with surface active properties. The secreted rhamnolipids were not utilised by S15 as a carbon source, but it caused a dispersion of bacterial biofilms in SBS medium. To the best of our knowledge, this is the first report on bioconversion of synthetic detergent to biodetergent. This SDS-based novel methodology presents a more economised mode of rhamnolipid synthesis utilising SDS as sole carbon source.

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Acknowledgment

The authors are grateful to Kerala State Council for Science Technology and Environment for funding and National Institute of Interdisciplinary Science and Technology (Thiruvananthapuram) for FAB-MS analysis.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, Kottayam, India

    Sharrel Rebello, Aju K. Asok & Jisha M.S.

  2. Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India

    Sunil V. Joseph, Biljo V. Joseph, Leny Jose & Sathish Mundayoor

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  1. Sharrel Rebello
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  2. Aju K. Asok
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  3. Sunil V. Joseph
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  4. Biljo V. Joseph
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  7. Jisha M.S.
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Correspondence to Jisha M.S..

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Rebello, S., Asok, A.K., Joseph, S.V. et al. Bioconversion of Sodium Dodecyl Sulphate to Rhamnolipid by Pseudomonas aeruginosa: A Novel and Cost-Effective Production Strategy. Appl Biochem Biotechnol 169, 418–430 (2013). https://doi.org/10.1007/s12010-012-9988-x

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