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Adsorption Equilibrium, Kinetics and Thermodynamics of α-Amylase on Poly(DVB-VIM)-Cu+2 Magnetic Metal-Chelate Affinity Sorbent

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Abstract

Designing an immobilised metal ion affinity process on large-scale demands that a thorough understanding be developed regarding the adsorption behaviour of proteins on metal-loaded gels and the characteristic adsorption parameters to be evaluated. In view of this requirement, interaction of α-amylase as a model protein with newly synthesised magnetic-poly(divinylbenzene-1-vinylimidazole) [m-poly(DVB-VIM)] microbeads (average diameter, 53–212 μm) was investigated. The m-poly(DVB-VIM) microbeads were prepared by copolymerising of divinylbenzene (DVB) with 1-vinylimidazole (VIM). The m-poly(DVB-VIM) microbeads were characterised by N2 adsorption/desorption isotherms, electron spin resonance, elemental analysis, scanning electron microscope and swelling studies. Cu2+ ions were chelated on the m-poly(DVB–VIM) beads and used in adsorption of α-amylase in a batch system. The maximum α-amylase adsorption capacity of the m-poly(DVB–VIM)–Cu2+ beads was determined as 10.84 mg/g at pH 6.0, 25 °C. The adsorption data were analyzed using three isotherm models, which are the Langmuir, Freundlich and Dubinin–Radushkevich isotherm models. The pseudo-first-order, pseudo-second-order, modified Ritchie’s-second-order and intraparticle diffusion models were used to test dynamic experimental data. The study of temperature effect was quantified by calculating various thermodynamic parameters such as Gibbs free energy, enthalpy and entropy changes.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Art, Uludag University, Bursa, Görükle Campus, Nilüfer, 16059, Turkey

    Bilgen Osman, Ali Kara, Emel Demirbel, Senay Kök & Necati Beşirli

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  1. Bilgen Osman
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  2. Ali Kara
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  3. Emel Demirbel
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  4. Senay Kök
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  5. Necati Beşirli
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Correspondence to Bilgen Osman.

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Osman, B., Kara, A., Demirbel, E. et al. Adsorption Equilibrium, Kinetics and Thermodynamics of α-Amylase on Poly(DVB-VIM)-Cu+2 Magnetic Metal-Chelate Affinity Sorbent. Appl Biochem Biotechnol 168, 279–294 (2012). https://doi.org/10.1007/s12010-012-9771-z

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  • DOI: https://doi.org/10.1007/s12010-012-9771-z

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