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Enhancement of the Activity and Enantioselectivity of Lipase by Sol–Gel Encapsulation Immobilization onto β-cyclodextrin-Based Polymer

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Abstract

Candida rugosa lipase was encapsulated within a chemically inert sol–gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of β-cyclodextrin-based polymer. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yield of the encapsulated lipase was 65 U/g, which is 7.5 times higher than that of the covalently immobilized lipase. The β-cyclodextrin-based encapsulated lipases had higher conversion and enantioselectivity compared with covalently immobilized lipase. The study confirms an excellent enantioselectivity (E >300) for the encapsulated lipase with an enantiomeric excess value of 98% for S-naproxen.

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Acknowledgment

The authors thank the Scientific Research Projects Foundation of Selcuk University (SUBAP grant number 08101024) for financial support of this work produced from a part of E. Yilmaz’s Ph.D. thesis.

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  1. Department of Chemistry, Faculty of Science, Selcuk University, Konya, 42075, Turkey

    Elif Yilmaz & Mehmet Sezgin

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  1. Elif Yilmaz
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Correspondence to Elif Yilmaz.

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Yilmaz, E., Sezgin, M. Enhancement of the Activity and Enantioselectivity of Lipase by Sol–Gel Encapsulation Immobilization onto β-cyclodextrin-Based Polymer. Appl Biochem Biotechnol 166, 1927–1940 (2012). https://doi.org/10.1007/s12010-012-9621-z

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