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Multienzymatic Sucrose Conversion into Fructose and Gluconic Acid through Fed-Batch and Membrane-Continuous Processes

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Abstract

Multienzymatic conversion of sucrose into fructose and gluconic acid was studied through fed-batch and continuous (in a membrane reactor) processes. The law of substrate addition (sucrose or glucose) for the fed-batch process which led to a yield superior to 80% was the decreasing linear type, whose feeding rate (ϕ; L/h) was calculated through the equation: ϕ = ϕo − k.t, where ϕo (initial feeding rate, L/h), k (linear addition constant, L/h 2), and t (reaction time, h). In the continuous process, the yield of conversion of sucrose (Y) was superior to 70% under the following conditions: dilution rate = 0.33 h−1, total duration of 15 h, pH 5.0, 37 °C and initial sucrose concentration of 64 g/L (Y = 92%), 100 g/L (Y = 83%), or 150 g/L (Y = 76%).

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References

  1. Goulas, A. K., Cooper, J. M., Grandison, A. S., & Rastall, R. A. (2004). Biotechnology and Bioengineering, 88, 778–779.

    Article  CAS  Google Scholar 

  2. Kennedy, J. F., Pimentel, M. C. B., Melo, E. H. M., & Lima-filho, J. L. (2007). Journal of the Science of Food and Agriculture, 87, 2266–2271.

    Article  CAS  Google Scholar 

  3. Vaz, A. J., Takei, K., & Bueno, E. C. (2007). Immunoassays: fundamentals and applications (Guanabara Koogan, ed). Rio de Janeiro, 67.

  4. Andreotti, D. Z., Tomotani, E. J., & Vitolo, M. (2010). European Biomass Conference and Exhibition from Research to Industry and Markets 18, 1367–1370. Lyon, France.

  5. Tomotani, E. J., Vitolo, M., Felipe, M. G. A., & Arruda, P. V. (2010). International Congress on Biocatalysis, 5, from 29/08 to 02/09/2010, Hamburg, Germany. Biocat, 2010, 103–104.

    Google Scholar 

  6. Panke, S., & Wubbolts, M. G. (2005). Current Opin Chem Biol, 9, 188–194.

    Article  CAS  Google Scholar 

  7. Sheu, D. C., Lio, P. J., Chen, S. T., Lin, C. T., & Duan, K. (2001). Biotechnology Letters, 23, 1499–1503.

    Article  CAS  Google Scholar 

  8. Tomotani, E. J., & Vitolo, M. (2010). Brazilian. Journal of Pharmaceutical Sciences, 46(3), 571–577.

    CAS  Google Scholar 

  9. Neves, L. C. M., & Vitolo, M. (2007). Applied Biochemistry and Biotechnology, 136, 161–170.

    Article  Google Scholar 

  10. Tomotani, E. J., & Vitolo, M. (2007). Enzyme and Microbial Technology, 40, 1020–1025.

    Article  CAS  Google Scholar 

  11. Tomotani, E. J., Neves, L. C. M., & Vitolo, M. (2005). Applied Biochemistry and Biotechnology, 121, 149–162.

    Article  Google Scholar 

  12. Sengupta, S., & Modak, J. M. (2001). Chemical Engineering Science, 56, 3315–3325.

    Article  CAS  Google Scholar 

  13. Pérez-terrazas, J. E., Ibarra-junquera, V., & Rosu, H. C. (2008). Korean J Chemical Engineer, 25(3), 461–465.

    Article  Google Scholar 

  14. Tomotani, E. J., & Vitolo, M. (2004). Applied Biochemistry and Biotechnology, 113, 145–159.

    Article  Google Scholar 

  15. Taraboulsi, F. A., Jr., & Vitolo, M. (2010). Analytica, 8(47), 86–93.

    Google Scholar 

  16. Da Silva, A. R. (2010). Dissertation. Sao Paulo, Brazil: University of São Paulo, School of Pharmaceutical Sciences.

    Google Scholar 

  17. Mansour, E. H., & Dawoud, M. (2003). Journal of the Science of Food and Agriculture, 83, 446–450.

    Article  CAS  Google Scholar 

  18. Arruda, L. M., & Vitolo, M. (1999). Applied Biochemistry and Biotechnology, 8, 23–33.

    Article  Google Scholar 

  19. Raba, J., & Mottola, H. A. (1995). Glucose oxidase as an analytical reagent. Critical Reviews in Analytical Chemistry, 25(1), 1–42.

    Article  CAS  Google Scholar 

  20. Fogarty, W. M., & Kelly, C. T. (1990). Microbial enzymes and biotechnology (2nd ed.). London: Elsevier Applied Science.

    Book  Google Scholar 

  21. Wigley, R. C. (1996). Cheese and Whey. In T. Godfrey & S. West (Eds.), Industrial Enzymology (2nd ed., pp. 135–154). London: MacMillan LTDA.

    Google Scholar 

  22. Echegaray, O. F., Carvalho, J. C. M., Fernandes, A. N. R., Sato, S., Aquarone, E., & Vitolo, M. (2000). Biomass and Bioenergy, 19, 39–50.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors acknowledge the financial grant from CNPq (Conselho Nacional de Pesquisa) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and thank Philip Barsanti for revising the manuscript.

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Authors and Affiliations

  1. University of São Paulo, School of Pharmaceutical Sciences, Av. Prof. Lineu Prestes, 580, B.16, 05508-900, São Paulo, SP, Brazil

    Fadi Antoine Taraboulsi Jr., Ester Junko Tomotani & Michele Vitolo

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  1. Fadi Antoine Taraboulsi Jr.
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  2. Ester Junko Tomotani
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  3. Michele Vitolo
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Correspondence to Fadi Antoine Taraboulsi Jr..

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Taraboulsi, F.A., Tomotani, E.J. & Vitolo, M. Multienzymatic Sucrose Conversion into Fructose and Gluconic Acid through Fed-Batch and Membrane-Continuous Processes. Appl Biochem Biotechnol 165, 1708–1724 (2011). https://doi.org/10.1007/s12010-011-9389-6

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