Abstract
The imperative role of functionalized natural alginate in immobilization of Lactobacillus delbrucekii (NCIM 2365) cells in production of optically pure l (+) lactic acid was studied. L. delbrucekii cells were immobilized in alginate, succinylated alginate and carrageenan to evaluate the bead stability and selectivity towards production of optically pure l (+) lactic acid. The scanning electron microscopic studies of free and immobilized cells show little morphological changes. The present study highlights the use of functionalized alginate-immobilized L. delbrucekii cells in production of l (+) lactic acid in higher yields (0.93 Yp/s in grams) with an improved enantioselectivity (99%). In addition, they further revealed decreased by-product formation (acetic and propionic acid) when compared to free and other immobilized cell fermentation.
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Acknowledgements
The authors of this investigation acknowledge Dr. Sanjay Nene, Scientist, NCL, Pune for providing L. delbrucekii culture, and Dr. Y Yogeshwara Rao and Dr. Meenakshi Singh of CSIR, New Delhi for financial support under NMITLI programme.
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Rao, C.S., Prakasham, R.S., Rao, A.B. et al. Functionalized Alginate as Immobilization Matrix in Enantioselective l (+) Lactic Acid Production by Lactobacillus delbrucekii . Appl Biochem Biotechnol 149, 219–228 (2008). https://doi.org/10.1007/s12010-007-8052-8
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DOI: https://doi.org/10.1007/s12010-007-8052-8