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Physical and Antimicrobial Properties of Compression-Molded Cassava Starch-Chitosan Films for Meat Preservation

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Abstract

Cassava starch-chitosan films were obtained by melt bending and compression molding, using glycerol and polyethylene glycol as plasticizers. Both the starch/chitosan and the polymer/plasticizer ratios were varied in order to analyze their effect on the physical properties of the films. Additionally, the antimicrobial activity of 70:30 polymer:plasticizer films was tested in cold-stored pork meat slices as affected by chitosan content. All film components were thermally stable up to 200 °C, which guaranteed their thermostability during film processing. Starch and chitosan had limited miscibility by melt blending, which resulted in heterogeneous film microstructure. Polyethylene glycol partially crystallized in the films, to a greater extent as the chitosan ratio increased, which limited its plasticizing effect. The films with the highest plasticizer ratio were more permeable to water vapor, less rigid, and less resistant to break. The variation in the chitosan content did not have a significant effect on water vapor permeability. As the chitosan proportion increased, the films became less stretchable, more rigid, and more resistant to break, with a more saturated yellowish color. The incorporation of the highest amount of chitosan in the films led to the reduction in coliforms and total aerobic counts of cold-stored pork meat slices, thus extending their shelf-life.

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References

  • Alves, V. D., Mali, S., Beleia, A., & Grossmann, M. V. (2007). Effect of glycerol and amylose enrichment on cassava starch film properties. Journal of Food Engineering, 78(3), 941–946.

    Article  CAS  Google Scholar 

  • ASTM (1995). Standard test methods for water vapour transmission of materials. In: Standards designations: E96-95. Annual book of ASTM standards (pp. 406-413). Philadelphia, PA: American Society for Testing and Materials.

  • ASTM (1999). Standard test method for specular gloss. In: Designation (D523). Annual book of ASTM standards, Vol. 06.01. Philadelphia, PA: American Society for Testing and Materials.

  • ASTM (2001). Standard test method for tensile properties of thin plastic sheeting. In: Standard D882 annual book of American standard testing methods. Philadelphia, PA: American Society for Testing and Materials.

  • Atarés, L., Bonilla, J., & Chiralt, A. (2010). Characterization of sodium caseinate-based edible films incorporated with cinnamon or ginger essential oils. Journal of Food Engineering, 100(4), 678–687.

    Article  CAS  Google Scholar 

  • Bonilla, J., Atarés, L., Vargas, M., & Chiralt, A. (2013). Properties of wheat starch film-forming dispersions and films as affected by chitosan addition. Journal of Food Engineering, 114(3), 303–312.

    Article  CAS  Google Scholar 

  • Bonilla, J., Fortunati, E., Atarés, L., Chiralt, A., & Kenny, J. (2014). Physical, structural and antimicrobial properties of poly vinyl alcohol-chitosan biodegradable films. Food Hydrocolloids, 35, 463–470.

    Article  CAS  Google Scholar 

  • Bourtoom, T., & Chinnan, M. S. (2008). Preparation and properties of rice starch–chitosan blend biodegradable film. LWT-Food Science and Technology, 41(9), 1633–1641.

    Article  CAS  Google Scholar 

  • Cano, A., Jiménez, A., Cháfer, M., González-Martínez, C., & Chiralt, A. (2014). Effect of amylose: amylopectin ratio and rice bran addition on starch films properties. Carbohydrate Polymers, 111(0), 543–555.

    Article  CAS  PubMed  Google Scholar 

  • Carvalho, A. J. F. (2008). Starch: Major sources, properties and applications as thermoplastic materials. In M. N. Belgacem & A. Gandini (Eds.), Monomers, polymers and composites from renewable resources. Amsterdam: Elsevier.

    Google Scholar 

  • Chillo, S., Flores, S., Mastromatteo, M., Conte, A., Gerschenson, L., & Del Nobile, M. A. (2008). Influence of glycerol and chitosan on tapioca starch-based edible film properties. Journal of Food Engineering, 88(2), 159–168.

    Article  CAS  Google Scholar 

  • Commission Regulation, 2005 (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. In Official Journal of the European Union pp 338/1–338/26.

  • Da Róz, A., Carvalho, A., Gandini, A., & Curvelo, A. (2006). The effect of plasticizers on thermoplastic starch compositions obtained by melt processing. Carbohydrate Polymers, 63(3), 417–424.

    Article  CAS  Google Scholar 

  • Dang, K., & Yoksan, R. (2015). Development of thermoplastic starch blown film by incorporating plasticized chitosan. Carbohydrate Polymers, 115, 575–581.

    Article  CAS  PubMed  Google Scholar 

  • Dou, B., Dupont, V., Williams, P. T., Chen, H., & Ding, Y. (2009). Thermogravimetric kinetics of crude glycerol. Bioresource Technology, 100(9), 2613–2620.

    Article  CAS  PubMed  Google Scholar 

  • Fang, J., Fawler, P., Eserig, C., González, R., Costa, J., & Chamudis, L. (2005). Development of biodegradable laminate films derived from naturally occurring carbohydrate polymers. Carbohydrate Polymers, 60(1), 39–42.

    Article  CAS  Google Scholar 

  • Hutchings, J. B. (1999). Food color and appearance (2nd ed.). Gaithersburg, Maryland, USA: Aspen Publishers, Inc..

    Book  Google Scholar 

  • Jiménez, A., Fabra, M. J., Talens, P., & Chiralt, A. (2012a). Edible and biodegradable starch films: A review. Food Bioprocessing Technology, 5(6), 2058–2076.

    Article  CAS  Google Scholar 

  • Jiménez, A., Fabra, M. J., Talens, P., & Chiralt, A. (2012b). Effect of re-crystallization on tensile, optical and water vapour barrier properties of corn starch films containing fatty acids. Food Hydrocolloids, 26(1), 302–310.

    Article  CAS  Google Scholar 

  • López, O., Garcia, A., Villar, M., Gentili, A., Rodriguez, M., & Albertengo, L. (2014). Thermo-compression of biodegradable thermoplastic corn starch films containing chitin and chitosan. LWT-Food Science and Technology, 57(106), 106–1515.

    Article  CAS  Google Scholar 

  • Mali, S., Grossmann, M. V. E., García, M. A., Martino, M. N., & Zaritsky, N. E. (2006). Effects of controlled storage on thermal, mechanical and barrier properties of plasticized films from different starch sources. Journal of Food Engineering, 75(4), 453–460.

    Article  CAS  Google Scholar 

  • Mendes, J. F., Paschoalin, R. T., Carmona, V. B., Sena Neto, A. R. A., Marques, C. P., Marconcini, J. M., Mattoso, L. H. C., Medeiros, E. S., & Oliveira, J. E. (2016). Biodegradable polymer blends based on corn starch and thermoplastic chitosan processed by extrusion. Carbohydrate Polymers, 137, 452–458.

    Article  CAS  PubMed  Google Scholar 

  • Ortega-Toro, R., Jiménez, A., Talens, P., & Chiralt, A. (2014). Properties of starch–hydroxypropyl methylcellulose based films obtained by compression molding. Carbohydrate Polymers, 109, 155–165.

    Article  CAS  PubMed  Google Scholar 

  • Ortega-Toro, R., Morey, I., Talens, P., & Chiralt, A. (2015). Active bilayer films of thermoplastic starch and polycaprolactone obtained by compression molding. Carbohydrate Polymers, 127, 282–290.

    Article  CAS  PubMed  Google Scholar 

  • Pelissari, F., Grossmann, M., Yamashita, F., & Pineda, E. (2009). Antimicrobial, mechanical and barrier properties of cassava starch-chitosan films incorporated with oregano essential oil. Journal of Agricultural and Food Chemistry, 57(16), 7499–7504.

    Article  CAS  PubMed  Google Scholar 

  • Pelissari, F. M., Yamashita, F., García, M. A., Martino, M. N., Zaritzky, N. E., & Grossmann, M. V. E. (2012). Constrained mixture design applied to the development of cassava starch-chitosan blown films. Journal of Food Engineering, 108(2), 262–267.

    Article  CAS  Google Scholar 

  • Song, R., Xue, R., He, L. H., Liu, Y., & Xiao, Q. L. (2008). The structure and properties of chitosan/polyethylene glycol/silica ternary hybrid organic-inorganic films. Chinese Journal of Polymer Science, 26(05), 621–630.v.

    Article  CAS  Google Scholar 

  • Su, J. F., Huang, Z., Yuan, X. Y., Wang, X. Y., & Lim, M. (2010). Structure and properties of carboxymethyl cellulose/soy protein isolate blend edible films crosslinked by Maillard reactions. Carbohydrate Polymers, 79(1), 145–153.

    Article  CAS  Google Scholar 

  • Thunwall, M., Boldizar, A., & Rigdahl, M. (2006). Compression molding and tensile properties of thermoplastic potato starch materials. Biomacromolecules, 7(3), 981–986.

    Article  CAS  PubMed  Google Scholar 

  • Tomé, L., Fernandes, S., Sadocco, P., Causio, J., Silvertre, A., Neto, P., & Freire, C. (2012). Antibacterial thermoplastic starch- chitosan based materials prepared by melt-mixing. BioResources, 7(3), 3398–3409.

    Google Scholar 

  • Villalobos, R., Chanona, J., Hernández, P., Gutiérrez, G., & Chiralt, A. (2005). Gloss and transparency of hydroxypropyl methylcellulose films containing surfactants as affected by their microstructure. Food Hydrocolloids, 19(1), 53–61.

    Article  CAS  Google Scholar 

  • Xu, Y. X., Kim, K. M., Hanna, M. A., & Nag, D. (2005). Chitosan–starch composite film: Preparation and characterization. Industrial Crops and Products, 21(2), 185–192.

    Article  CAS  Google Scholar 

  • Yang, L., & Paulson, A. T. (2000). Mechanical and water vapour barrier properties of edible gellan. Food Research International, 33(7), 563–570.

    Article  CAS  Google Scholar 

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Funding

The authors acknowledge the financial support provided by the Spanish Ministerio de Economía y Competividad (Projects AGL2013-42989-R and AGL2016-76699-R). Author Cristina Valencia-Sullca thanks the Peruvian Grant National Program (PRONABEC Grant).

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Correspondence to Maria Vargas.

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Valencia-Sullca, C., Atarés, L., Vargas, M. et al. Physical and Antimicrobial Properties of Compression-Molded Cassava Starch-Chitosan Films for Meat Preservation. Food Bioprocess Technol 11, 1339–1349 (2018). https://doi.org/10.1007/s11947-018-2094-5

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  • DOI: https://doi.org/10.1007/s11947-018-2094-5

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