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Microstructural and Photoacoustic Infrared Spectroscopic Studies of Human Cortical Bone with Osteogenesis Imperfecta

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Abstract

The molecular basis of bone disease osteogenesis imperfecta (OI) and the mineralization of hydroxyapatite in OI bone have been of significant research interest. To further investigate the mechanism of OI disease and bone mineralization, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and x-ray diffraction (XRD) are used in the present study to describe the structural and compositional differences between OI and healthy bone. OI bone exhibits more porous, fibrous features, abnormal collagen fibrils, and abnormal mineral deposits. Likewise, photoacoustic-FTIR experiments indicate an aberrant collagen structure and an altered mineral structure in OI. In contrast, there is neither significant difference in the non-collagenous proteins (NCPs) composition observed nor apparent change in the crystal structure between OI and healthy bone minerals as shown in XRD and energy-dispersive x-ray spectroscopy (EDS) results. This observation indicates that the biomineralization process is more controlled by the bone cells and non-collagenous phosphorylated proteins. The present study also confirms that there is an orientational influence on the stoichiometry of the mineral in OI bone. Also, a larger volume of the hydrated layer in the transverse plane than the longitudinal plane of the mineral crystal structure is proposed. The appearance of a new C–S band in the FTIR spectra in OI bone suggests the substitution of glycine by cysteine in collagen molecules or/and an increased amount of cysteine-rich osteonectin that relates to mineral nucleation and mineral crystal formation.

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Acknowledgements

Instrumentation obtained from National Science Foundation MRI grants is acknowledged for enabling experiments conducted in this work. The authors would like to acknowledge the assistance in electron microscopy laboratory from Mr. Scott Payne. Author CG would like to acknowledge the support from Doctoral Dissertation Award of NDSU graduate school.

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  1. Department of Civil and Environmental Engineering, North Dakota State University, Fargo, ND, 58105, USA

    Chunju Gu, Dinesh R. Katti & Kalpana S. Katti

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  1. Chunju Gu
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Correspondence to Kalpana S. Katti.

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Gu, C., Katti, D.R. & Katti, K.S. Microstructural and Photoacoustic Infrared Spectroscopic Studies of Human Cortical Bone with Osteogenesis Imperfecta. JOM 68, 1116–1127 (2016). https://doi.org/10.1007/s11837-016-1838-9

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