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Fibromatoses desmoïdes: étude immunohistochimique de 14 cas sur tissue microarray (TMA)

Desmoid fibromatosis: immunohistochemical analysis of 14 cases on tissue microarray (TMA)

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Bio tribune magazine

Résumé

Introduction

Les fibromatoses desmoïdes sont des tumeurs bénignes rares, localement agressives, ayant tendance à récidiver mais ne disséminant pas à distance. Le diagnostic histologique peut être difficile. Un complément immunohistochimique est alors utile.

Notre objectif a été d’étudier le profil immunohistochimique de ces tumeurs pour évaluer l’aide diagnostique que peut apporter cette technique.

Matériels et méthodes

14 cas de fibromatoses extra-abdominales sporadiques ont été étudiés. Ces cas ont été re grou - pés sur 1 bloc de tissue-microarray, et des immunomarquages utilisant les marqueurs β caténine, APC (antigène de la polypose adénomateuse colique), WT1, CD117, récepteurs oestrogéniques et progestéroniques ont été réalisés.

Résultats

Une expression nucléaire de la β-caténine est retrouvée dans tous les cas testés (100%). La protéine APC est exprimée dans 11 cas sur 14 (78,6 %). Par contre, les récepteurs oestrogéniques et progestéroniques, le CD117 et le WT1 sont négatifs.

Nos résultats confirment le rôle de la voie de signalisation Wnt/β-catenine/APC, dont la dérégulation joue un rôle crucial dans le développement des tumeurs desmoïdes.

Abstract

Background/aim

Desmoid fibromatosis are rare, benign but locally aggressive tumors, characterized by an infiltrative growth and a tendency towards local recurrence, but an inability to metastasise. The morphological diagnosis may be difficult, requiring immunohistochemistry.

The aim of our study is to determine the im munohistochemical phenotypes of these tumours to evaluate if they are helpful and to define a diagnostic strategy.

Methods

Immunohistochemistry was used to examine the expression of β-catenin, APC protein, in archival material derived from fourteen cases of extraabdominal desmoid tumors.

Desmoids specimens were assembled into a clinical data-linked tissue micro — array. Nuclear β-catenin expression was observed in 100% of the specimens. Positive cytoplasmic staining for APC protein was found in 11 of 14 (78,6%). But all samples were negative for oestrogen and progesterone receptors, c-KIT and WT1.

Results

Our results regarding β-catenin and APC confirm the previous findings that those proteins play a crucial role in the pathogenesis of sporadic aggressive fibromatosis.

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Authors and Affiliations

  1. Institut National d’Oncologie (INO), Rabat, Maroc

    H. Kadiri, M.A Belabbas & M. Amrani

  2. Department of Experimental Oncology, Mediterranean Institute of Oncology, Catania, Italy

    L. Memeo

  3. Department of Pathology, Columbia University Medical Center, New York, NY, USA

    M. Mansukhani

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  1. H. Kadiri
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  2. L. Memeo
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  3. M.A Belabbas
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  4. M. Mansukhani
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  5. M. Amrani
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Correspondence to M. Amrani.

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Kadiri, H., Memeo, L., Belabbas, M. et al. Fibromatoses desmoïdes: étude immunohistochimique de 14 cas sur tissue microarray (TMA). Bio trib. mag. 36, 36–43 (2010). https://doi.org/10.1007/s11834-010-0020-x

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