Abstract
WRKY proteins are known to play major roles in defense signaling. We identified a pathogen-inducible and SA-inducible OsWRKY30. Our cDNA clone encodes the C-terminal region (CTR) of OsWRKY30. CTR-OsWRKY30 includes the C-terminal WRKY domain and nuclear localization sequence. CTR-OsWRKY30 was sufficient to bind W-box sequences (TTGACC/T). Over-expression of the CTR-OsWRKY30 resulted in enhanced resistance to pathogens in Arabidopsis and rice. Defense-related genes were constitutively expressed in transgenic Arabidopsis and rice over-expressing CTR-OsWRKY30. Based on promoter transient assays, CTR-OsWRKY30 is sufficient to activate OsPR10a promoter as much as full length OsWRKY30. Taken together, CTR-OsWRKY30 positively regulates defense signaling, thereby resulting in enhanced resistance to pathogens.
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Abbreviations
- ET:
-
Ethylene
- JA:
-
Jasmonic acid
- Pcc :
-
Pectobacterium carotovora ssp. carotovora
- SA:
-
Salicylic acid
- Xcc :
-
Xanthomonas campestris pv. campestris
- Xoo :
-
Xanthomonas oryzae pv. oryzae
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Acknowledgment
This work was supported in part by two grants (PJ007850 and PJ008574) from Rural development administration to Dr. Duk-Ju Hwang.
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H. Lee and Y.J. Ko equally contributed to this work.
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11816_2012_252_MOESM1_ESM.ppt
Supplementary Fig. 1. Phylogenetic tree of OsWRKY30 with the other WRKY proteins. It is constructed by clustal W of Megalign program in DNA STAR package. C in OsWRKY53C, AtWRKY33C, OsWRKY30C, and AtWRKY25C stands for C-terminal WRKY domain. Distance in the tree corresponds to evolutionary distance. Locus identifiers of the genes from this article are as follows: At1g62300 (WRKY6), At1g18860 (WRKY61), At4g31800 (WRKY18), At2g25000 (WRKY60), At3g01970 (WRKY45), At5g64810 (WRKY51), At2g30250 (WRKY25), At4g24240 (WRKY7), At2g23320 (WRKY15), At4g31550 (WRKY11), At4g01250 (WRKY22), At4g23550 (WRKY29), At1g66560 (WRKY64), At3g56400 (WRKY70), OsWRKY53 (AK121190), OsWRKY30 (AK066518) (PPT 73 kb)
11816_2012_252_MOESM2_ESM.ppt
Supplemental Fig. 2. Alignment of OsWRKY30 with related WRKY proteins. Three Group I WRKY proteins were aligned by clustal W of Megalign in DNA STAR package. Identical amino acids are in pink box between them (PPT 180 kb)
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Lee, H., Ko, Y.J., Cha, JY. et al. The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes. Plant Biotechnol Rep 7, 221–230 (2013). https://doi.org/10.1007/s11816-012-0252-1
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DOI: https://doi.org/10.1007/s11816-012-0252-1