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Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose

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Abstract

Rugosa rose (Rosa rugosa) is cultivated as a garden flower and an important genetic resource for the breeding of roses (R. hybrida). This study describes culture conditions for high frequency plant regeneration from zygotic embryo explants via somatic embryogenesis in rugosa rose. Mature zygotic embryo, cotyledon, and radicle explants formed embryogenic calluses at frequencies of 38, 6.7, and 8.8% when cultured on half-strength Murashige and Skoog medium (½MS) supplemented with 2.26, 9.05, and 9.05 μM 2,4-dichlorophenoxyacetic acid, respectively. Embryogenic calluses produced numerous somatic embryos, which then developed into plantlets on ½MS without growth regulators. Regenerated plantlets were grown to whole plants in a growth chamber.

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Acknowledgments

This work was supported by a grant (ABC1000912) to S.W.K. from BioGreen 21 Program funded by the Rural Development Administration, a grant to J.R.L. from the Crop Functional Genomics Center of the 21st Century Frontier Research Program, a grant to J.R.L. from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of the Kyung Hee University funded by the Korea Ministry of Education, Science and Technology, and a grant to J.R.L. from the Marine Extreme Genome Research Center funded by the Korean Ministry of Marine Affairs and Fisheries.

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Authors and Affiliations

  1. Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea

    Suk Weon Kim

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea

    Myung Jin Oh & Jang R. Liu

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  1. Suk Weon Kim
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  2. Myung Jin Oh
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  3. Jang R. Liu
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Correspondence to Jang R. Liu.

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Kim, S.W., Oh, M.J. & Liu, J.R. Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnol Rep 3, 199–203 (2009). https://doi.org/10.1007/s11816-009-0092-9

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  • DOI: https://doi.org/10.1007/s11816-009-0092-9

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