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High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

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Abstract

An improved protocol for high frequency plant regeneration via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield (Brasenia schreberi) was developed. Zygotic embryos formed pale-yellow globular structures and white friable callus at a frequency of 80% when cultured on half-strength MS medium supplemented with 0.3 mg l−1 2,4-D. However, the frequency of formation of pale-yellow globular structures and white friable callus decreased slightly with increasing concentrations of 2,4-D up to 3 mg l−1, where the frequency reached ~50% of the control. Cell suspension cultures from zygotic embryo-derived white friable callus were established using half-strength MS medium supplemented with 0.3 mg l−1 2,4-D. Upon plating of cell aggregates on half-strength MS basal medium, approximately 8.3% gave rise to somatic embryos and developed into plantlets. However, the frequency of plantlet development from cell aggregates was sharply increased (by up to 55%) when activated charcoal and zeatin were applied. Regenerated plantlets were successfully transplanted to potting soil and grown to normal plants in a growth chamber. The distinctive feature of this study is the establishment of a high frequency plant regeneration system via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield, which has not been previously reported. The protocol for plant regeneration of watershield through somatic embryogenesis could be useful for the mass propagation and transformation of selected elite lines.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog

NAA:

α-Naphthaleneacetic acid

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Acknowledgments

This work was supported by a grant from KRIBB Research Initiative Program, and a grant from the Korea Science and Engineering Foundation (BDW0800723) and the Korea Institute of Environment Science and Technology (EGW0600712).

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Authors and Affiliations

  1. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yuseong-gu, Daejeon, 305-806, South Korea

    Myung Jin Oh & Suk Weon Kim

  2. Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoeun-dong, Yuseong-gu, Daejeon, 305-806, South Korea

    Jang Ryol Liu

  3. Department of Biological Science, Ajou University, Suwon, 443-749, South Korea

    Hye Ryun Na & Hong-Keun Choi

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  1. Myung Jin Oh
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  2. Hye Ryun Na
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  3. Hong-Keun Choi
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  4. Jang Ryol Liu
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  5. Suk Weon Kim
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Correspondence to Suk Weon Kim.

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Oh, M.J., Na, H.R., Choi, HK. et al. High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi). Plant Biotechnol Rep 2, 87–92 (2008). https://doi.org/10.1007/s11816-008-0047-6

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  • DOI: https://doi.org/10.1007/s11816-008-0047-6

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