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Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption

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Abstract

SBA-15-NH2 and SBA-15-Cl Mesoporous Materials were prepared by modifying SBA-15 with silane coupling agent 3-aminopropyltriethoxysilane and 3-chloropropyltriethoxysilane using the post-synthesis method. The mesoporous samples were characterized by Fourier transform infrared spectra and nitrogen adsorption. Compared with SBA-15-NH2, SBA-15-Cl has suitable pore opening for further utilization in the immobilization of Candida sp.99-125 lipase by physical adsorption. The influences of lipase concentration and immobilizing time on the immobilization efficiency were investigated. Meanwhile, the lipase immobilized on SBA-15-Cl showed higher thermal, pH and storage stability than that of free lipase. Further study demonstrated that lipase immobilized on SBA-15-Cl could be used eight times without significant decrease of enzyme activity. The phenomenon was associated with the shrinkage of the pore opening of SBA-15-Cl keeping lipase from leaping out.

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, Jiangsu, 210009, China

    Ya Li, Wei Wang & Pingfang Han

  2. Department of Environment and Resource, Nantong Agricultural College, Nantong, Jiangsu, 226007, China

    Ya Li

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  1. Ya Li
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  2. Wei Wang
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  3. Pingfang Han
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Correspondence to Pingfang Han.

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Li, Y., Wang, W. & Han, P. Immobilization of Candida sp.99-125 lipase onto silanized SBA-15 mesoporous materials by physical adsorption. Korean J. Chem. Eng. 31, 98–103 (2014). https://doi.org/10.1007/s11814-013-0198-1

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  • DOI: https://doi.org/10.1007/s11814-013-0198-1

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