Abstract
Calcium carbonate is one of the most abundant materials present in nature. Crystal structures of CaCO3 become three polymorphic modifications, namely calcite, aragonite and vaterite. Polymorphic modifications are mediated by adding aspartic acid (Asp) and lysozyme. Lysozyme, which is a major component of egg white proteins, has influenced the calcification of avian eggshells. The influence of Asp and lysozyme on the crystallization of CaCO3 was investigated by adding these additives and calcium chloride solution into sodium carbonate solution in a crystallization vessel. CaCO3 crystals were analyzed by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectrometry (FT-IR). XRD was used to select the intensities and crystal structure of specific calcium carbonate. SEM was employed for the analysis of the morphology of the precipitation and particle size. Two kinds of crystals were identified by FT-IR spectrum. Hexagonal crystals of vaterite were affected by the Asp in the crystallization solution. However, rhombohedral crystals of calcite by lysozyme were formed without any sign of vaterite.
Similar content being viewed by others
References
C. Shivkumara, P. Singh, A. Gupta and M. S. Hegade, Mater. Res. Bull., 41, 1455 (2006).
K. Tamura and H. Tsuge, Chem. Eng. Sci., 61, 5818 (2006).
X. Sun, Y. Zhou, J. Ren, F. Cui and H. Li, Appl. Phys., 7, 75 (2007).
S. Bentov, S. Weil, L. Glazer and A. Berman, J. Struct. Biol., 171(2), 207 (2010).
L. Gago-Duport, M. J. I. Briones, J. B. Rodriguez and B. Covelo, J. Struct. Biol., 162(3), 422 (2008).
M. Faatz, F. Grohn and G. Wegner, Mater. Sci. Eng., 25(2), 153 (2005).
R. Beck and J. Andreassen, Cryst. Growth, 312(15), 2226 (2010).
W. M. Jung, S. H. Kang, W. S. Kim and C. K. Choi, Chem. Eng. Sci., 55(4), 733 (2000).
S.H. Kang, I. Hirasawa, W. S. Kim and C. K. Choi, J. Colloid Interface Sci., 288(2), 496 (2005).
H. Wei, Q. Shen, T. Zhao, D. Wang and D. Xu., Cryst. Growth, 260(3), 545 (2004).
J. Kawano, N. Shimobayashi, M. Kitamura, K. Shinoda and N. Aikawa, J. Cryst. Growth, 237, 419 (2002).
Y. Li, T. Wiliana and K. C. Tam, Mater. Res. Bull., 42(5), 820 (2007).
G. Hadiko, Y. S. Han, M. Fuji and M. Takahashi, Mater. Lett., 59(19), 25192 (2005).
Y. S. Han, G. Hadiko, M. Fuji and M. Takahashi, J. Cryst. Growth, 289(1), 269 (2006).
D. Zhao, T. Zhu, F. Li, Q. Ruan, S. Zhang, L. Zhang and D. Xu, Mater. Res. Bull., 45(1), 80 (2010).
J. Aizenberg, S. Albeck, S. Weiner and L. Addadi, J. Cryst. Growth, 142(1), 156 (1994).
H. Wentao and F. Qingling, Mater. Sci. Eng., 26(4), 644. (2006).
G. E. Henderson and K. M. McGrath, J. Crystal Growth, 244(3), 369 (2002).
Y. Hu, Y. Ma, Y. Zhou, F. Nie, X. Duan and C. Pei, J. Cryst. Growth, 321(10), 1741 (2010).
G. Frenandez and E. Castro, J. Food Eng., 92(1), 112 (2009).
P. S. Lukeman, W. B. Sherman, C. Micheel, A. P. Alivisatos and N. C. Seeman, Biophys. J., 95(1), 3340 (2008).
J. H. Kim, J. M. Kim, W. S. Kim and I. H. Kim, Korean Chem. Eng. Res., 47(2), 213 (2009).
J. H. Kim, S. M. Song, J. M. Kim, W. S. Kim and I. H. Kim, Korean J. Chem. Eng., 27(5), 1535 (2010).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Song, S.M., Kim, I.H. Biomineralization of calcium carbonate by adding aspartic acid and lysozyme. Korean J. Chem. Eng. 28, 1749–1753 (2011). https://doi.org/10.1007/s11814-011-0022-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-011-0022-8