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Preparations, characterizations and applications of chitosan-based nanoparticles

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Abstract

Chitosan is a natural polysaccharide prepared by the N-deacetylation of chitin. In this paper we have reviewed the methods of preparation of chitosan-based nanoparticles and their pharmaceutical applications. There are five methods of their preparations: emulsion cross-linking, emulsion-droplet coalescence, ionic gelation, reverse micellar method and chemically modified chitosan method. Chitosan nanoparticles are used as carriers for low molecular weight drug, vaccines and DNA. Releasing characteristics, biodistribution and applications are also summarized.

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References

  • Chen, X. G., C. M. Lee, and H. J. Park, 2003. O/W emulsification for the self-aggregation and nanoparticle formation of linoleic acids modified chitosan in the aqueous system. J. Agric. Food Chem., 51: 3135–3139.

    Article  Google Scholar 

  • Christopher, P. L., and S. L. Philip., 2001. Folate-mediated targeting: from diagnostics to drug and gene delivery. Drug Discov. Today, 6: 44–51.

    Article  Google Scholar 

  • Corsi, K., F. Chellat, L. Yahia, and J. C. Fernandes, 2003. Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. Biomaterials, 24: 1255–1264.

    Article  Google Scholar 

  • Cui, Z., and R. J. Mumper, 2001. Chitosan-based nanoparticles for topical genetic immunization. J. Control. Release, 75: 409–419.

    Article  Google Scholar 

  • Dyer, A. M., M. Hinchcliffe, P. Watts, J. Castile, I. Jabbal-Gill, et al., 2002. Nasal delivery of insulin using novel chitosan based formulations: a comparative study in two animal models between simple chitosan formulations and chitosan nanoparticles. Pharma. Res., 19: 998–1008.

    Article  Google Scholar 

  • Erbacher, P., S. Zou, T. Bettinger, A. M. Steffan, and J. S. Remy, 1998. Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability. Pharma. Res., 159: 1332–1339.

    Article  Google Scholar 

  • Felt, O., P. Buri, and R. Gurny, 1998. Chitosan: a unique polysaccharide for drug delivery. Drug Devel. Indus. Pharm., 24: 979–993.

    Article  Google Scholar 

  • Fernandez-Urrusuno, R., P. Cavlo, C. Remunan-Lopez, J. L. Vila-Jato, and M. J. Alonso, 1999. Enhancement of nasal absorption of insulin using chitosan nanoparticles. Pharm. Res., 16: 1576–1581.

    Article  Google Scholar 

  • Gao, S., J. Chen, X. Xu, Z. Ding, Y.H. Yang, et al., 2003. Galactosylated low molecular weight chitosan as DNA carrier for hepatocyte-targeting. Int. J. Clin. Pharm. Res., 255: 57–68.

    Google Scholar 

  • Illum, L., I. Jabbal-Gill, M. Hinchcliffe, A. N. Fisher, and S. S. Davis, 2001. Chitosan as a novel nasal delivery system for vaccines. Adv. Drug Deliv. Rev., 51: 81–96.

    Article  Google Scholar 

  • Janes, K. A., P. Calvo, and M. J. Alonso, 2001a. Polysaccharide colloidal particles as delivery systems for macromolecules. Adv. Drug Deliv. Rev., 47: 83–97.

    Article  Google Scholar 

  • Janes, K. A., M. P. Fresneau, M. Ana, F. Angels, and J. A. Marıa, 2001b. Chitosan nanoparticles as delivery systems for doxorubicin. J. Control. Release, 73: 255–267.

    Article  Google Scholar 

  • Jong, H. K., S. K. Yoo, K. W. Sung, J. H. Park, K. Kim, et al., 2006. Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel. J. Control. Release, 111: 228–234.

    Article  Google Scholar 

  • Kataoka, K., T. Matsumoto, M. Tokohama, T. Okano, Y. Sakurai, et al., 2000. Doxorubicin-loaded poly(ethylene glycol)-poly(b-benzyl-L-aspartate) copolymer micelles: their pharmaceutical characteristics and biological significance. J. Control. Release, 64: 143–153.

    Article  Google Scholar 

  • Kim, T. H., I. K. Park, J. W. Nah, Y. J. Choi and C. S. Cho, 2004. Galactosylated chitosan/DNA nanoparticles prepared using water-soluble chitosan as a gene carrier. Biomaterials, 25: 3 783–3 792.

    Google Scholar 

  • Lee, K. Y., J. H. Kim, I. C. Kwon, and S. Y. Jeong, 2001. Self-aggregates of deoxycholic acid-modified chitosan as a novel carrier of adriamycin. Coll. Polym. Sci., 278: 1216–1219.

    Article  Google Scholar 

  • Liu, C. G., K. G. H. Desai, X. G. Chen, and H. J. Park, 2005a. Linolenic Acid-Modified Chitosan for Formation of Self-Assembled Nanoparticles. J. Agric. Food Chem., 53: 437–441.

    Article  Google Scholar 

  • Liu, C. G., X. G. Chen, and H. J. Park, 2005b. Self-assembled nanoparticles based on linoleic-acid modified chitosan: Stability and adsorption of trypsin. Carbohyd. Polym., 62: 293–298.

    Article  Google Scholar 

  • MacLaughlin, F. C., R. J. Mumper, J. Wang, J. M. Tagliaferri, I. Gill, et al., 1998. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery. J. Control. Release, 56: 259–272.

    Article  Google Scholar 

  • Mao, H. Q., K. Roy, V. L. Troung-Le, K. Y. Lim, Y. Wang, et al., 1997. DNA-chitosan nanospheres: derivitization and storage stability. P. Int. Symp. Control. Release. Bioact. Mater., 24: 671–672.

    Google Scholar 

  • Mao, H. Q., K. Roy, V. L. Troung-Le, K. A. Janes, K. Y. Lim, et al., 2001. Chitosan DNA nanoparticles as gene delivery carriers: synthesis, characterization and transfection efficiency. J. Control. Release, 70: 399–421.

    Article  Google Scholar 

  • Mitra, S., U. Gaur, P. C. Ghosh, and A. N. Maitra, 2001. Tumor targeted delivery of encapsulated dextran-doxorubicin conjugate using chitosan nanoparticles as carrier. J. Control. Release, 74: 317–323.

    Article  Google Scholar 

  • Murata, J., Y. Ohya and T. Ouchi, 1996. Possibility of application of quaternary chitosan having pendant galactose residues as gene delivery tool. Carbohyd. Polym., 29: 69–74.

    Article  Google Scholar 

  • Ohya, Y., M. Shiratani, H. Kobayashi, and T. Ouchi, 1994. Release behavior of 5-fluorouracil from chitosan-gel nanospheres immobilizing 5-fluorouracil coated with polysaccharides and their cell specific cytotoxicity. Pure Appl. Chem., A31: 629–642.

    Google Scholar 

  • Park, I. K., T. H. Kim, Y. H. Park, B. A. Shin, E. S. Choi, et al., 2001. Galactosylated chitosan-graft-poly (ethylene glycol) as hepatocyte-targeting DNA carrier. J. Control. Release, 76: 349–362.

    Article  Google Scholar 

  • Park, J. H., S. Kwon, M. Lee, H. Chung, H. K. Ji, et al., 2006, Self-assembled nanoparticles based on glycol chitosan bearing hydrophobic moieties as carriers for doxorubicin: In vivo biodistribution and anti-tumor activity. Biomaterials, 27: 119–126.

    Article  Google Scholar 

  • Richardson, S. C. W., H. V. J. Kolbe, and R. Dunean, 1999. Potential of low molecular mass chitosan as a DNA delivery system: biocompatibility, body distribution and ability to complex and protect DNA. Int. J. Pharm., 178: 231–243.

    Article  Google Scholar 

  • Roy, K., H. Q. Mao, and K. W. Leong, 1997. DNA-chitosan nanosperes: transfection effiency and cellar uptake. Preoceeding Int. Symp. Control. Release. Bioact. Mater., 24: 673–674.

    Google Scholar 

  • Sania, M., C. Yan, W. Francoise, S. Qin, L. Patrick, et al., 2006. Characterization of folate-chitosan-DNA nanoparticles for gene therapy. Biomaterials, 27: 2060–2065.

    Article  Google Scholar 

  • Sato, T., T. Ishii and Y. Okahata, 2001. In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency. Biomaterials, 22: 2075–2080.

    Article  Google Scholar 

  • Se, K. K., and R. Niranjan, 2005. Enzymatic production and biological activities of chitosan oligosaccharides (COS): A review. Carbohyd. Polym., 62: 357–368.

    Article  Google Scholar 

  • Sevda, S., and S. J. McClureb, 2004. Potential applications of chitosan in veterinary medicine. Adv. Drug Deliver. Rev., 56: 1467–1480.

    Article  Google Scholar 

  • Son, Y. J., J. S. Jang, Y. W. Cho, H. Chung, R. W. Park, et al., 2003. Biodistribution and anti-tumor efficacy of doxorubicin loaded glycol-chitosan nanoaggregates by EPR effect. J. Control. Release, 91: 135–145.

    Article  Google Scholar 

  • Sunil, A. A., N. M. Nadagouda, and M. Tejraj, 2004. Recent advances on chitosan-based micro-and nanoparticles in drug delivery. J. Control. Release, 100: 5–28.

    Article  Google Scholar 

  • Takeuchi, H., H. Yamamoto, and Y. Kawashima, 2001. Mucoadhesive nanoparticle systems for peptide drug delivery. Adv. Drug Deliver. Rev., 47: 39–54.

    Article  Google Scholar 

  • Thanou, M., B. I. Florea, M. Geldof, H. E. Junginger and G Borchard, 2002. Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines. Biomaterials, 23: 153–159.

    Article  Google Scholar 

  • Tokumitsu, H., H. Ichikawa, and Y. Fukumori, 1999. Chitosan-gadopentetic acid complex nanoparticles for gadolinium neutron capture therapy of cancer: preparation by novel emulsiondroplet coalescence technique and characterization. Pharm. Res., 16: 1830–1835.

    Article  Google Scholar 

  • Uchegbu, I. F., A. G. Schatzlein, L. Tetley, A. I. Gray, J. Sludden, et al., 1998. Polymeric chitosan-based vesicles for drug delivery. J. Pharm. Pharmacol., 50: 453–458.

    Google Scholar 

  • Van der Lubben, I. M., J. C. Verhoef, G. Borchard, and H. E. Junginger, 2001. Chitosan and its derivatives in mucosal drug and vaccine delivery. Eur. J. Pharm. Sci., 14: 201–207.

    Article  Google Scholar 

  • Van der Lubben, I. M., J. C. Verhoef, G. Borchard, and H. E. Junginger, 2001. Chitosan for mucosal vaccination. Adv. Drug Deliver. Rev., 52: 139–144.

    Article  Google Scholar 

  • Vila, A., A. Sa–nchez, K. Janes, I. Behrens, T. Kissel, et al., 2004. Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice. Eur. J. Pharm. Biopharm., 57: 123–131.

    Article  Google Scholar 

  • Wang, S., J. Luo, D. A. Lantrip, D. J. Waters, C. J. Mathias, et al., 1997. Design and synthesis of DTPA-folate for use as a tumortargeted radiopharmaceutical. Bioconjugate Chem., 8: 673–679.

    Article  Google Scholar 

  • Xu, Y. M., Y. M. Du, R. H. Huang, and L. P. Gao, 2003. Preparation and modification of N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride nanoparticle as a protein carrier. Biomaterials, 24: 5015–5022.

    Article  Google Scholar 

  • Yokohama, M., S. Fukushima, R. Uehara, K. Okamoto, K. Kataoka, et al., 1998. Characterization of physical entrapment and chemical conjugation of adriamycin in polymeric micelles and their design for in vivo delivery to a solid tumor. J. Control. Release, 50: 79–92.

    Article  Google Scholar 

  • Yoo, H. S., and T. G. Park, 2004a. Folate receptor targeted biodegradable polymeric doxorubicin micelles. J. Control. Release, 96: 273–283.

    Article  Google Scholar 

  • Yoo, H. S., and T. G. Park, 2004b. Folate-receptor-targeted delivery of doxorubicin nano-aggregates stabilized by doxorubicin-PEG-folate conjugate. J. Control. Release, 100: 247–256.

    Article  Google Scholar 

  • Yoo, H. S., J. E. Lee, H. Chung, I. C. Kwon, and S. Y. Jeong, 2005. Self-assembled nanoparticles containing hydrophobically modified glycol chitosan for gene delivery. J. Control. Release, 103: 235–243.

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, Qingdao, 266003, P. R. China

    Liu Chenguang, Tan Yulong, Liu Chengsheng, Chen Xiguang & Yu Lejun

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  1. Liu Chenguang
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  2. Tan Yulong
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  3. Liu Chengsheng
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  4. Chen Xiguang
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  5. Yu Lejun
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Correspondence to Liu Chenguang.

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Liu, C., Tan, Y., Liu, C. et al. Preparations, characterizations and applications of chitosan-based nanoparticles. J Ocean Univ. China 6, 237–243 (2007). https://doi.org/10.1007/s11802-007-0237-9

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  • DOI: https://doi.org/10.1007/s11802-007-0237-9

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