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An extraction-assay system: Evaluation on flavonols in plant resistance to Pb and Cd by supercritical extraction-gas chromatography

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Abstract

In this research, supercritical carbon dioxide extraction (SFE) showed better extraction effect when compared with Solid- liquid extraction (SLE), Soxhlet extraction (SE) and Ultrasonic extraction (UE), not only in the rate but also the time. The comparison among these three extraction modifiers, including acetone, ethanol and methanol demonstrated that ethanol was preferred to SFE due to its high extraction effect and low toxicology. In addition, parameter of SFE, influence of temperature and pressure were investigated, and the best extraction effect was achieved at the optima conditions, temperature of 40°C and the pressure of 35 MPa. Thus, SFE is a highly effective method for flavonols extraction, requiring minimum energy and producing non-toxic byproduct. SFE-GC system is applied for the evaluation on flavonols that plays a key role in plant resistance to heavy metal, with its content and synthetase gene expression significantly increasing in plant when threatened by heavy metal. Besides, results indicated that flavonols can improve plant resistance to oxidative stress by quenching the redundant ROS in matrix.

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References

  • Benaventegarcía O, Castillo J (2008). Update on uses and properties of citrus flavonoids: New findings in anticancer, cardiovascular, and anti-inflammatory activity. Journal of Agricultural and Food Chemistry, 56(15): 6185–6205

    Article  CAS  Google Scholar 

  • Bogdanov M G, Svinyarov I, Keremedchieva R, Sidjimov A (2012). Ionic liquid-supported solid‒liquid extraction of bioactive alkaloids. I. New HPLC method for quantitative determination of glaucine in Glaucium flavum Cr. (Papaveraceae). Separation and Purification Technology, 97: 221–227

    CAS  Google Scholar 

  • Dauner M, Sauer U (2000). GC-MS Analysis of amino acids rapidly provides rich information for isotopomer balancing. Biotechnology Progress, 16(4): 642–649

    Article  CAS  Google Scholar 

  • Diet A, Link B, Seifert G J, Schellenberg B, Wagner U, Pauly M, Reiter W D, Ringli C (2006). The Arabidopsis root hair cell wall formation mutant LRX1 is suppressed by mutations in the RHM1 gene encoding a UDP-l-rhamnose synthase. Plant Cell, 18(7): 1630–1641

    Article  CAS  Google Scholar 

  • El-Aty A M A, Choi J H, Ko M W, Khay S, Goudah A, Shin H C, Kim J S, Chang B J, Lee C H, Shim J H (2009). Approaches for application of sub and supercritical fluid extraction for quantification of orbifloxacin from plasma and milk: Application to disposition kinetics. Analytica Chimica Acta, 631(1): 108–115

    Article  CAS  Google Scholar 

  • Emteborg H, Björklund E, Ödman F, Karlsson L, Mathiasson L, Frech W, Baxter D C (2016). Determination of methylmercury in sediments using supercritical fluid extraction and gas chromatography coupled with microwave-induced plasma atomic emission spectrometry. Analyst (London), 121(1): 19–29

    Article  Google Scholar 

  • Espinosa-Pardo F A, Nakajima V M, Macedo G A, Macedo J A, Martínez J (2016). Extraction of phenolic compounds from dry and fermented orange pomace using supercritical CO2 and cosolvents. Food and Bioproducts Processing, 101: 1–10

    Article  CAS  Google Scholar 

  • Ferrentino G, Calliari N, Bertucco A, Spilimbergo S (2014). Validation of a mathematical model for predicting high pressure carbon dioxide inactivation kinetics of Escherichia coli spiked on fresh cut carrot. Journal of Supercritical Fluids, 85: 17–23

    Article  CAS  Google Scholar 

  • Hossein K, Karamatollah R (2009). Effect of various parameters on the selective extraction of main components from hyssop using supercritical fluid extraction (SFE). Food Science and Technology Research, 15(6): 645–652

    Article  Google Scholar 

  • Houben R J, Janssen H G M, Leclercq P A, Rijks J A, Cramers C A (2015). Supercritical fluid extraction-capillary gas chromatography: On-line coupling with a programmed temperature vaporizer. Journal of Separation Science, 13: 669–673

    Google Scholar 

  • Hu Y, Li C, Kulkarni B A, Strobel G, Lobkovsky E, Torczynski RMJr P J (2001). Exploring chemical diversity of epoxyquinoid natural products: Synthesis and biological activity of (-)-jesterone and related molecules. Organic Letters, 3(11), 1649–1652

    Article  CAS  Google Scholar 

  • Jaakola L, Hohtola A (2010). Effect of latitude on flavonoid biosynthesis in plants. Plant, Cell & Environment, 33: 1239

    CAS  Google Scholar 

  • Jaramillo K, Dawid C, Hofmann T, Fujimoto Y, Osorio C (2011). Identification of antioxidative flavonols and anthocyanins in Sicana odorifera fruit peel. Journal of Agricultural and Food Chemistry, 59 (3): 975–983

    Article  CAS  Google Scholar 

  • Jungbluth G, Ternes W (2000). HPLC separation of flavonols, flavones and oxidized flavonols with UV-, DAD-, electrochemical and ESI-ion trap MS detection. Fresenius’ Journal of Analytical Chemistry, 367 (7): 661–666

    Article  CAS  Google Scholar 

  • Kamali H, Ghaziaskar H S, Khakshour A, Kaboudvand M (2013). Supercritical CO2 extraction of phthalic anhydride, benzoic acid and maleic acid from petrochemical wastes. Journal of Supercritical Fluids, 74: 46–51

    Article  CAS  Google Scholar 

  • Katherine L S, Edgar C C, Jerry W K, Luke R H, Julie C D (2008). Extraction conditions affecting supercritical fluid extraction (SFE) of lycopene from watermelon. Bioresource Technology, 99(16): 7835–7841

    Article  CAS  Google Scholar 

  • Kazazi H, Rezaei K, Ghotb-Sharif S J, Emam-Djomeh Z, Yamini Y (2007). Supercriticial fluid extraction of flavors and fragrances from Hyssopus officinalis L. cultivated in Iran. Food Chemistry, 105(2): 805–811

    Article  CAS  Google Scholar 

  • Kuhn B M, Geisler M, Bigler L, Ringli C (2011). Flavonols accumulate asymmetrically and affect auxin transport in Arabidopsis. Plant Physiology, 156(2): 585–595

    Article  CAS  Google Scholar 

  • Kumar P, Pal A, Saxena M K, Ramakumar K L (2008). Supercritical fluid extraction of uranium and thorium from solid matrices. Desalination, 232(1-3): 71–79

    Article  CAS  Google Scholar 

  • Kutchko B G, Goodman A L, Rosenbaum E, Natesakhawat S, Wagner K (2013). Characterization of coal before and after supercritical CO2 exposure via feature relocation using field-emission scanning electron microscopy. Fuel, 107: 777–786

    Article  CAS  Google Scholar 

  • Lummaetee K, Ku H M, Wongrat W, Elkamel A (2017). Optimization of supercritical fluid extraction of isoflavone from soybean meal. Canadian Journal of Chemical Engineering, 95(6): 1141–1149

    Article  CAS  Google Scholar 

  • Maran J P, Manikandan S, Priya B, Gurumoorthi P (2015). Box-Behnken design based multi-response analysis and optimization of supercritical carbon dioxide extraction of bioactive flavonoid compounds from tea (Camellia sinensis L.) leaves. Journal of Food Science and Technology, 52(1): 92–104

    Article  CAS  Google Scholar 

  • Mason J T, Chemat F, Vinatoru M (2016). The extraction of natural products using ultrasound or microwaves. Current Organic Chemistry, 15: 237–247

    Article  Google Scholar 

  • Modey W K, Mulholland D A, Raynor M W (2015). Analytical supercritical fluid extraction of natural products. Phytochemical Analysis, 7(1): 1–15

    Article  Google Scholar 

  • Pan H, Zhang X, Ren B, Yang H, Ren Z, Wang W (2017). Toxic assessment of cadmium based on online swimming behavior and the continuous AChE activity in the gill of Zebrafish (Danio rerio). Water, Air, and Soil Pollution, 228(9): 355

    Article  CAS  Google Scholar 

  • Rajagopalan R, Vaucheret H, Trejo J, Bartel D P (2006). A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes & Development, 20(24): 3407–3425

    Article  CAS  Google Scholar 

  • Ren Z (2015). Characterizing response behavior of medaka (Oryzias latipes) under chemical stress based on self-organizing map and filtering by integration. Ecological Informatics, 29: 107–118

    Article  Google Scholar 

  • Shen J, Shao X (2005). A comparison of accelerated solvent extraction, Soxhlet extraction, and ultrasonic-assisted extraction for analysis of terpenoids and sterols in tobacco. Analytical and Bioanalytical Chemistry, 383(6): 1003–1008

    Article  CAS  Google Scholar 

  • Skerget M, Kotnik P, Hadolin M, Hras A R, Simonic M, Knez Z (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89(2): 191–198

    Article  CAS  Google Scholar 

  • Spence A J, Jimenez-Flores R, Qian M, Goddik L (2009). The influence of temperature and pressure factors in supercritical fluid extraction for optimizing nonpolar lipid extraction from buttermilk powder. Journal of Dairy Science, 2009, 92(2): 458–468

    CAS  Google Scholar 

  • Sung S, Amasino R M (2004). Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3. Nature, 427(6970): 159–164

    Article  CAS  Google Scholar 

  • Suzuki N, Koussevitzky S, Mittler R, Miller G (2012). ROS and redox signalling in the response of plants to abiotic stress. Plant, Cell & Environment, 35(2): 259–270

    Article  CAS  Google Scholar 

  • Taher H, Al-Zuhair S, Al-Marzouqi A H, Haik Y, Farid M (2014). Mass transfer modeling of Scenedesmus sp. lipids extracted by supercritical CO2. Biomass and Bioenergy, 70: 530–541

    Article  CAS  Google Scholar 

  • Veggi P C, Cavalcanti R N, Meireles M A A (2011). Modifier effects on supercritical fluid extraction (SFE) of some Brazilian plants: Antioxidant activity and economical evaluation. Procedia Food Science, 1: 1717–1724

    Article  CAS  Google Scholar 

  • Wang W, Dong C, Dong W, Yang C, Ju T, Huang L, Ren Z (2016). The design and implementation of risk assessment model for hazard installations based on AHP–FCE method: A case study of Nansi Lake Basin. Ecological Informatics, 36: 162–171

    Article  CAS  Google Scholar 

  • Xing N, Ji L, Song J, Ma J, Li S, Ren Z, Xu F, Zhu J (2017). Cadmium stress assessment based on the electrocardiogram characteristics of zebra fish (Danio rerio): QRS complex could play an important role. Aquatic Toxicology (Amsterdam, Netherlands), 191: 236–244

    Article  CAS  Google Scholar 

  • Yang C, Ming Z, Dong W, Cui G, Ren Z, Wang W (2017). Highly efficient photocatalytic degradation of methylene blue by PoPD/TiO2 nanocomposite. PLoS One, 12(3): e0174104

    Article  CAS  Google Scholar 

  • Yin L, Yang H, Si G, Ren Q, Fu R, Zhang B, Zhang X, Wang X, Qi P, Xia C (2015). Persistence parameter: A reliable measurement for behavioral responses of Medaka (Oryzias latipes) to Environmental Stress. Environmental Modeling and Assessment, 21: 1–9

    CAS  Google Scholar 

  • Ren Z, Zhang X, Wang X, Qi P, Zhang B, Zeng Y (2015). AChE inhibition: One dominant factor for swimming behavior changes of Daphnia magna under DDVP exposure. Chemosphere, 120, 252

    Article  CAS  Google Scholar 

  • Zhang Q, Li Y, Phanlavong P, Wang Z, Jiao T, Qiu H, Peng Q (2017a). Highly efficient and rapid fluoride scavenger using an acid/base tolerant zirconium phosphate nanoflake: Behavior and mechanism. Journal of Cleaner Production, 161: 317–326

    Article  CAS  Google Scholar 

  • Zhang Q, Li Y, Yang Q, Chen H, Chen X, Jiao T, Peng Q (2017b). Distinguished Cr(VI) capture with rapid and superior capability using polydopamine microsphere: Behavior and mechanism. Journal of Hazardous Materials, 342: 732–740

    Article  CAS  Google Scholar 

  • Zhang X, Li X, Yang H, Cui Z (2018a). Biochemical mechanism of phytoremediation process of lead and cadmium pollution with Mucor circinelloides and Trichoderma asperellum. Ecotoxicology and Environmental Safety, 157: 21–28

    Article  CAS  Google Scholar 

  • Zhang X, Ren B, Li S, Qu X, Yang H, Xu S, Ren Z, Kong Q, Wang C (2017c). Is sodium percarbonate a good choice in situ remediation of deltamethrin pollution? Frontiers of Environmental Science & Engineering, 11(3): 3

    Article  CAS  Google Scholar 

  • Zhang X, Yang H, Cui Z (2016). A new indicator to evaluate the pollution of iron and manganese. RSC Advances, 6(33): 27963–27968

    Article  CAS  Google Scholar 

  • Zhang X, Yang H, Cui Z (2017d). Alleviating effect and mechanism of flavonols in Arabidopsis resistance under Pb–HBCD stress. ACS Sustainable Chemistry & Engineering, 5(11): 11034–11041

    Article  CAS  Google Scholar 

  • Zhang X, Yang H, Cui Z (2018b). Evaluation and analysis of soil migration and distribution characteristics of heavy metals in iron tailings. Journal of Cleaner Production, 172: 475–480

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to School of Life Sciences in Shandong University for their suggestions and support to improve the basic research. Special thanks are extended to Department of Plant and Microbial Biology in University of Zurich for technical support and article modification. Many thanks to CSC (Xu Zhang was Supported by China Scholarship Council for 13 months study at University of Zurich).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China

    Xu Zhang, Xinlei Wang, Wen Song & Zhaojie Cui

  2. School of Life Science, Shandong University, Jinan, 250100, China

    Huanhuan Yang

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  1. Xu Zhang
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Correspondence to Zhaojie Cui.

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Zhang, X., Yang, H., Wang, X. et al. An extraction-assay system: Evaluation on flavonols in plant resistance to Pb and Cd by supercritical extraction-gas chromatography. Front. Environ. Sci. Eng. 12, 6 (2018). https://doi.org/10.1007/s11783-018-1059-2

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