Abstract
The N2O production in two nitrogen removal processes treating domestic wastewater was investigated in laboratory-scale aerobic-anoxic sequencing batch reactors (SBRs). Results showed that N2O emission happened in the aerobic phase rather than in the anoxic phase. During the aerobic phase, the nitrogen conversion to N2O gas was 27.7%and 36.8% of NH +4 -N loss for conventional biologic N-removal process and short-cut biologic N-removal process. The dissolved N2O was reduced to N2 in the anoxic denitrification phase. The N2O production rate increased with the increasing of nitrite concentration and ceased when NH +4 -N oxidation was terminated. Higher nitrite accumulation resulted in higher N2O emission in the short-cut nitrogen removal process. Pulse-wise addition of 20 mg NO −2 -N·L−1 gave rise to 3-fold of N2O emission in the conventional N-removal process, while little change happened with 20 mg NO −3 -N·L−1 was added to SBR1.
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Noda N, Kaneko N, Mikami M, Kimochi Y, Tsuneda S, Hirata A, Mizuochi M, Inamori Y. Effects of SRT and DO on N2O reductase activity in an anoxic-oxic activated sludge system. Water Science & Technology, 2003, 48(11–12): 363–370
Liu X H, Peng Y, Wu C Y, Akio T, Peng Y Z. Nitrous oxide production during nitrogen removal from domestic wastewater in lab-scale sequencing batch reactor. Journal of Environmental Sciences (China), 2008, 20(6): 641–645
Zhou Y, Pijuan M, Yuan Z G. Development of a 2-sludge, 3-stage system for nitrogen and phosphorous removal from nutrient-rich wastewater using granular sludge and biofilms. Water Research, 2008, 42(12): 3207–3217
Intergovernmental Panel on Climate Change (IPCC). Climate Change: the Scientific Basis. Cambridge: Cambridge University Press, 2001
Kampschreur M J, Temmink H, Kleerebezem R, Jetten M S M, Loosdrecht M C M. Nitrous oxide emission during wastewater treatment. Water Research, 2009, 43(17 ):4093–4103
Bock E, Koops H P, Harns H. Cell Biology of Nitrifying Bacteria, Nitrification. Oxford: IRL Press, 1986, 17–38
Prosser J I. Autotrophic nitrification in bacteria. Advances in Microbial Physiology, 1989, 30(1): 125–181
von Schulthess R, Wild D, Gujer W. Nitric and nitrous oxides from denitrifying activated sludge at low oxygen concentration. Water Science & Technology, 1994, 30(6): 123–132
Osada T, Kuroda K, Yonaga M. Reducing nitrous oxide gas emissions from fill-and-draw type activated sludge process. Water Research, 1995, 29(6): 1607–1608
Itokawa H, Hanaki K, Matsuo T. Nitrous oxide production in high-loading biological nitrogen removal process under low COD/N ratio condition. Water Research, 2001, 35(3): 657–664
Burgess J E, Colliver B B, Stuetz R M, Stephenson T. Dinitrogen oxide production by a mixed culture of nitrifying bacteria during ammonia shock loading and aeration failure. Journal of Industrial Microbiology & Biotechnology, 2002, 29(6): 309–313
Peng Y Z, Zhu G B. Biological nitrogen removal with nitrification and denitrification via nitrite pathway. Applied Microbiology and Biotechnology, 2006, 73(1): 15–26
Bock E, Schmidt I, Stüven R, Zart D. Nitrogen loss caused by denitrifying Nitrosomonas cells using ammonium or hydrogen as electron donors and nitrite as electron acceptor. Archives of Microbiology, 1995, 163(1): 16–20
Kampschreur M J, Tan N C G, Kleerebezem R, Picioreanu C, Jetten MS M, Loosdrecht MCM. Effect of dynamic process conditions on nitrogen oxides emission from a nitrifying culture. Environmental Science & Technology, 2008, 42(2): 429–435
Zeng R J, Lemaire R, Yuan Z, Keller J. A novel wastewater treatment process: simultaneous nitrification, denitrification and phosphorus removal. Water Science &Technology, 2004, 50(10): 163–170
Lemaire R, Meyer R, Taske A, Crocetti G R, Keller J, Yuan Z. Identifying causes for N2O accumulation in a lab-scale sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal. Journal of Biotechnology, 2006, 122(1): 62–72
American Public Health Association (APHA). Standard Methods for the Examination for Water and Wastewater, 20th ed. Washington, D C: American Public Health Association, 1998
Henze M, Gujer W, Mino T, van Loosdrecht M C M. Activated Sludge Models ASM1, ASM2, ASM2d and ASM3. IWA Scientific and Technical Report No.9. London: IWA Publishing, 2000
Yang Q, Liu X, Peng C Y, Wang S, Sun H, Peng Y. N2O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method. Environmental Science & Technology, 2009, 43(24): 9400–9406
Ahn J H, Kwan T, Chandran K. Comparison of partial and full nitrification processes applied for treating high-strength nitrogen wastewaters: microbial ecology through nitrous oxide production. Environmental Science & Technology, 2011, 45(7): 2734–2740
Colliver B B, Stephenson T. Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnology Advances, 2000, 18(3): 219–232
Wrage N, Velthol G L, van Beausichem M L, Oenema O. Role of nitrifier denitrification in the production of nitrous oxide. Soil Biology and Biochemistry, 2001, 33(12–13): 1723–1732
Kampschreur M J, van der Star W R L, Wielders H A, Mulder J W, Jetten MS M, van Loosdrecht MC M. Dynamics of nitric oxide and nitrous oxide emission during full-scale reject water treatment. Water Research, 2008, 42(3): 812–826
Wicht H. A model for predicting nitrous oxide production during denitrification in activated sludge. Water Science & Technology, 1996, 34(5–6): 99–106
Tallec G, Garnier J, Billen G, Gousailles M. Nitrous oxide emissions from denitrifying activated sludge of urban wastewater treatment plants, under anoxia and low oxygenation. Bioresource Technology, 2008, 99(7): 2200–2209
Nelson L M, Knowles R. Effect of oxygen and nitrate on nitrogen fixation and denitrification by Azospirillum brasilense grown in continuous culture. Canadian Journal of Microbiology, 1978, 24(11): 1395–1403
Sacks L E, Barker H A. The influence of oxygen on nitrate and nitrite reduction. Journal of Bacteriology, 1949, 58(1): 11–22
Otte S, Grobben N G, Robertson L A, Jetten M S M, Kuenen J G. Nitrous oxide production by Alcaligenes faecalis under transient and dynamic aerobic and anaerobic conditions. Applied and Environmental Microbiology, 1996, 62(7): 2421–2426
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Gong, Y., Peng, Y., Wang, S. et al. Production of N2O in two biologic nitrogen removal processes: a comparison between conventional and short-cut nitrogen removal processes. Front. Environ. Sci. Eng. 8, 589–597 (2014). https://doi.org/10.1007/s11783-013-0571-7
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DOI: https://doi.org/10.1007/s11783-013-0571-7