Abstract
Biological hydrogen production by dark fermentation is an important part of biological hydrogen production technologies. China is a typical developing country that heavily relies on fossil fuels; thus, new, clean, and sustainable energy development turns quite urgent. It is delightful that Chinese government has already drawn up several H2 development policies since 1990s and provided financial aid to launch some H2 development projects. In this paper, the research status on dark fermentative hydrogen production in China was summarized and analyzed. Subsequently, several new findings and achievements, with some of which transformed into scale-up tests, were highlighted. Moreover, some prospecting coupling processes with dark fermentation of hydrogen production were also proposed to attract more research interests in the future.
Similar content being viewed by others
References
Ren N Q, Wang B Z, Huang J C. Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor. Biotechnol Bioeng, 1997, 54: 428–433
Guo W Q, Ren N Q, Wang X J, Xiang W S, Meng Z H, Ding J, Qu Y Y, Zhang L S. Biohydrogen production rate from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor. Int J Hydrogen Energy, 2008, 33(19): 4981–4988
Wang X J, Ren N Q, Xiang W S. Effect on iron on hydrogen production capacity, hydrogenase and NADH-Fd reductase activities of a fermentative hydrogen producing bacteria strain B49. High Technol Lett, 2004, 10(4): 69–74
Xing D F, Ren N Q, Rittmann B E. Genetic diversity of hydrogen-producing bacteria in an acidophilic ethanol-H2-coproducing system, analyzed using the [Fe]-hydrogenase gene. App Environ Microbiol, 2008, 74(4): 1232–1239
Li Y F, Ren N Q, Yang C P, Li J Z, Wang X Z, Li P. The molecular characterization and traits of a high efficient biohydrogen-producing bacterium in clostridium genus. Acta Energiae Solaris Sinica, 2006, 27(2): 208–211 (in Chinese)
Chen S Y, Dong X Z. Characterization of a new clostridium species and its hydrogen production. Acta Microbiologica Sinica, 2004, 44(4): 411–416 (in Chinese)
Xing D F, Ren N Q, Li Q B, Lin M, Wang A J, Zhao L H. Ethanoligenens harbinensegen. nov., sp nov., isolated from molasses wastewater. Int J Syst Evol Microbiol, 2006, 56: 755–760
Tanisho S, Shiwata Y. Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenesis. Int J Hydrogen Energy, 1994, 9: 807–812
Ren N Q, Wang B Z, Ma F. Hydrogen bio-production of carbohydrate fermentation by anaerobic activated sludge process. In: Proceedings of the 68th Water Environment Federation Annual Conference Exposure. Miami: 1995, 145–153
Ren N Q, Lin M, Ma X P, Wang A J, Li J Z. A strain of anaerobic bacteria screened for high efficient hydrogen production and its aciduric character. Acta Energiae Solaris Sinica, 2003, 24(1): 80–84 (in Chinese)
Xu L Y, Ren N Q, Wang X Z, Jia Y F. Biohydrogen production by Ethanoligenens harbinenseB49: Nutrient optimization. Int J Hydrogen Energy, 2008, 33(23): 6962–6967
Guo W Q, Ren N Q, Wang X J, Xiang W S, Ding J, You Y, Liu B F. Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology. Bioresour Technol, 2009, 100: 1192–1196
Zhu D L, Wang G C, Qiao H J, Cai J L. Fermentative hydro gen production by the new marine Pantoea agglomerans isolated from the mangrove sludge. Int J Hydrogen Energy, 2008, 33: 6116–6123
Ren N Q, Ding J, Ding L, Liu M, Li YF, Bao H X. Effect of Cu2+ concentration on hydrogen fermentation by mixed culture. Harbin Institute of Technol (New Series), 2004, 11(1): 11–16
Hao X L, Zhou M H, Yu H Q, Shen Q Q, Lei L C. Effect of sodium ion concentration on hydrogen production from sucrose by anaerobic hydrogen-producing granular sludge. Chinese J Chem Eng, 2006, 14(4): 511–517 (in Chinese)
Wang X J, Ren N Q, Xiang W S, Guo W Q. Influence of gaseous end-products inhibition and nutrient limitations on the growth and hydrogen production by hydrogen-producing fermentative bacterial B49. Int J Hydrogen Energy, 2007, 32: 748–754
Wang J L, Wan W. Influence of Ni2+ concentration on biohydrogen production. Bioresour Technol, 2008, 99: 8864–8868
Lin C Y, Lay C H. Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora. Int. J Hydrogen Energy, 2004, 29(1): 41–45
Lee K S, Lin P J, Fangchiang K, Chang J S. Continuous hydrogen production by anaerobic mixed microflora using a hollow-fiber microfiltration membrane bioreactor. Int J Hydrogen Energy, 2007, 32: 950–957
Li J Z, Li B K, Zhu G F, Ren N Q, Bo L X, He J G. Hydrogen production from diluted molasses by anaerobic hydrogen producing bacteria in an anaerobic baffled reactor (ABR). Int J Hydrogen Energy, 2007, 32: 3274–3283
Ren N Q, Wang X J, Xiang W S, Lin M, Li J Z, Guo W Q. Hydrogen production with high yield by immobilized cells of hydrogen-producing bacteria strain B49 in a column reactor. High Technol Lett, 2002, 8(4): 21–25
Chang J S, Lee K S, Lin P J. Biohydrogen production with fixed-bed bioreactors. Int J Hydrogen Energy, 2002, 27: 1167–1174
Yu H Q, Zhu Z H, Hu W R, Zhang H S. Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures. Int J Hydrogen Energy, 2002, 27: 1359–1365
Guo W Q, Ren N Q, Chen Z B, Liu B F, Wang X J, Xiang W S. Simultaneous biohydrogen production and starch wastewater treatment in an acidogenic expanded granular sludge bed reactor by mixed culture for long-term operation. Int J Hydrogen Energy, 2008, 33: 7397–7404
Fang H H P, Liu H. Effect of pH on hydrogen production from glucose by a mixed culture. Bioresource Technol, 2002, 82: 87–93
Wu S Y, Hung C H, Lin C N, Chen H W, Lee A S, Chang J S. Fermentative hydrogen production and bacterial community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge. Biotechnol Bioeng, 2005, 93(5): 934–946
Lee K S, Wu J F, Lo Y S, Lo Y C, Lin P J, Chang J S. Anaerobic hydrogen production with an efficient carrier-induced granular sludge bed bioreactor. Biotechnol Bioeng, 2004, 87, 648-657
Wu K J, Chang J S. Batch and continuous fermentative production of hydrogen with anaerobic sludge entrapped in a composite polymeric matrix. Process Biochem, 2007, 42: 279–284
Ren N Q, Cao G L, Wang A J, Lee D J, Guo W Q, Zhu Y H. Dark fermentation of xylose and glucose mix using isolated Thermoanaerobacterium thermosaccharolyticum W16. Int J Hydrogen Energy, 2008, 33(21): 6124–6132
Ren N Q, Wang A J, Gao L F, Xin L, Lee D J, Su A. Bioaugmented hydrogen production from carboxymethyl cellulose and partially delignified corn stalks using isolated cultures. Int J Hydrogen Energy, 2008, 33(19): 5250–5255
Lu Y, Lai Q H, Zhang C, Zhao H X, Ma K, Zhao X B, Chen H Z, Liu D H, Xing X H. Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. Bioresour Technol, 2009, 100: 2889–2895
Li D, Yuan Z H, Sun Y M, Kong X Y, Zhang Y. Hydrogen production characteristics of the organic fraction of municipal solid wastes by anaerobic mixed culture fermentation. Int J Hydrogen Energy, 2009, 34(2): 812–820
Ren N Q, Zheng G X. Screening and H2-producing behavior of highly efficient H2-producing mutant UV-d48. J Chem Industry and Eng, 2007, 58(3): 755–758 (in Chinese)
Ren N Q, Lin H L, Zhang K, Zheng G X, Duan Z J, Lin M. Cloning, expression, and characterization of an acetate kinase from a high rate of biohydrogen bacterial strain Ethanoligenens sp. hit B49. Current Microbiol, 2007, 55: 167–72
Author information
Authors and Affiliations
Corresponding author
Additional information
Dr. Nanqi Ren is a National Distinguished Young Scholar of China, Chang-Jiang Scholars Professor, and Head of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (HIT). He focuses on environmental biotechnology, bio-energy technology of H2, CH4, and electricity, biological processes of wastewater treatment, and POPs in water, air, and soil. He invented a technology of biohydrogen production from ethanol fermentation, found a novel genus of hydrogen-producing bacteria (Ethanoligenens), and established a full-scale biohydrogen production plant. He got two times of National Second-Place Award by China, published more than 300 papers and 9 books, and held more than 20 patents.
Rights and permissions
About this article
Cite this article
Ren, N., Guo, W., Liu, B. et al. Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects. Front. Environ. Sci. Eng. China 3, 375–379 (2009). https://doi.org/10.1007/s11783-009-0148-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11783-009-0148-7