Skip to main content
SpringerLink
Log in

Morphology of the reproductive tract of females of leaf beetle Chrysomela populi (Chrysomelidae: Coleoptera)

  • Original Article
  • Published:
Biologia Aims and scope Submit manuscript

Abstract

In this study, oocyte development and vitellogenesis of the leaf beetle Chrysomela populi are described and illustrated using light and scanning electron microscopies. The female reproductive tract of C. populi consists of a pair of ovaries, a pair of lateral oviducts, a common oviduct and a spermathecae. Each ovary has 14 telotrophic meroistic ovarioles. Each ovariole consists of a terminal filament, germarium, vitellarium and pedicel. Terminal filament is thin, long thread shaped. The germarium contains trophocytes towards the distal part, prefollicular cells and oocytes towards the proximal part in the young stage. The vitellarium contains 3–4 oocytes at different stages of development (previteloogenic, vitellogenic and choriogenic oocytes). Previtellogenic oocytes located towards the distal end of the vitellarium are surrounded by follicle epithelium. Vitellogenic oocytes are surrounded by a monolayer follicular epithelium, and their ooplasma is basophilic. The ooplasma of the oocytes in the choriogenic stage, which is the last stage of vitellogenesis, is filled with eosinophilic yolk granules of different sizes. Chorion is clearly differentiated between the vitelline membrane surrounding the ooplasma and the follicular epithelium. The pedicel connects the ovarioles with the lateral oviduct. A pair of lateral oviducts are connected to the common oviduct. A spermatheca is located towards the proximal part of the common oviduct. Spermatheca consists of a reservoir, duct and gland. The eggs are light yellow in color and the chorion is very thin. This study intends to contribute to female reproductive biology studies in Coleoptera and other insect order species, agricultural struggle studies in terms of researching C. populi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aslan I, Özbek H (1999) Faunistic and systematic studies on the subfamily Chrysomelinae (Coleoptera, Chrysomelidae) in Artvin, Erzincan and Erzurum provinces of Turkey. Turk J Zool 23:751–767

    Google Scholar 

  • Aslan GE, Kaya ÖD, Ünal E (2020) Contributions to the knowledge of leaf beetle (Coleoptera: Chrysomelidae) fauna in Elazığ, Erzincan and Tunceli provinces. Journal of Graduate School of Natural and Applied Sciences of Mehmet Akif Ersoy University 11(Suppl Issue 1):273–280. https://doi.org/10.29048/makufebed.783029

  • Assis MQ, Dohanik VT, de Oliveira LL, Zanuncio JC, Serrão JE (2019) Evidence for a transcellular route for vitellogenin transport in the telotrophic ovary of Podisus nigrispinus (Hemiptera: Pentatomidae). Sci Rep 9(1):1–8. https://doi.org/10.1038/s41598-019-52789-z

    Article  CAS  Google Scholar 

  • Boonyoung P, Senarat S, Kettratad J, Jiraungkoorskul W, Thaochan N, Sing KW, Pengsakul P, Poolprasert P (2020) Mature gonadal histology and gametogenesis of the tortoise beetle Aspidimorpha sanctaecrucis (Fabricius, 1792) (Coleoptera: Cassidinae: Chrysomelidae): Histological observation. Songklanakarin J Sci Technol 42(4):725–947

    Google Scholar 

  • Büning J (1994) The insect ovary: ultrastructure, previtellogenic growth and evolution. First edn. Chapman & Hall, London

    Book  Google Scholar 

  • Caron E, Ribeiro-Costa CS, Linzmeier AM (2004) The egg morphology of some species of Sennius Bridwell (Coleoptera: Chrysomelidae: Bruchinae) based on scanning electron micrographs. Zootaxa 556(1):1–10

    Article  Google Scholar 

  • Cavalcaselle B (1972) Pilot experiments for the control of insect pests of poplar in the nursery by means of systemic insecticides in granular form. Celulosa e Carta 23:17–25

    CAS  Google Scholar 

  • Cheetham T (1992) Anatomy and histology of tissues associated with egg deposition in Chrysomela scripta Fabr. (Coleoptera: Chrysomelidae). J Kans Entomol Soc 65(2):101–114

    Google Scholar 

  • Devasahayam S, Vidyasagar PSPV, Koya KM (1998) Reproductive system of pollu beetle, Longitarsus nigripennis Motschulsky (Coleoptera: Chrysomelidae), a major pest of black pepper, Piper nigrum Linnaeus. J Entomol Res 22(1):77–82

    Google Scholar 

  • Georgiev G (2000) Species composition and impact of the phytophagous insects on the poplars (Populus spp.) in Bulgaria. Nauka za Gorata 37:45–54

    Google Scholar 

  • Gerber GH, Neill GB, Westdal PH (1978) The anatomy and histology of the internal reproductive organs of the sunflower beetle, Zygogramma exclamationis (Coleoptera: Chrysomelidae). Can J Zool 56(12):2542–2553

    Article  Google Scholar 

  • Goldson SL, Emberson RM (1981) Reproductive morphology of the Argentine stem weevil, Hyperodes bonariensis (Coleoptera: Curculionidae). N Z J Zool 8(1):67–77

    Article  Google Scholar 

  • Izumi S, Yano K, Yamamoto Y, Takahashi SY (1994) Yolk proteins from insect eggs: structure, biosynthesis and programmed degradation during embryogenesis. J Insect Physiol 40:735–746

    Article  CAS  Google Scholar 

  • Jolivet P (2015) Together with 30 years of symposia on Chrysomelidae! Memories and personal reflections on what we know more about leaf beetles. In: Jolivet P, Santiago-Blay J, Schmitt M (eds) Research on Chrysomelidae 5. Zookeys, pp 35–61

  • Jolivet P, Petipierre E, Hasiao TH (1988) Biology of chrysomelidae. Series entomologica, 42, Kluver Academic Puplishers, Dordrecht

  • Kasap H (1988) A list of some Chrysomelinae (Col., Chrysomelidae) from Turkey. II. Türk Entomol Derg 12:85–95

    Google Scholar 

  • Khan MA, Ahmad D (1991) Comparative toxicity of some chlorinated hydrocarbon insecticides to poplar leaf beetle–Chrysomela populi L. (Coleoptera: Chrysomelidae). Indian J For 14:42–45

    CAS  Google Scholar 

  • Klowden MJ (2009) Spermatheca. In: Vincent HR, Ring TC (eds) Encyclopedia of insects, 2nd edn. Academic, Burlington, pp 939–940

    Chapter  Google Scholar 

  • Konstantinov AS, Korotyaev BA, Volkovitsh MG (2009) Insect biodiversity in the Palearctic region. In: Foottit RG, Adler PH (eds) Insect biodiversity: science and society. Wiley Blackwell, Oxford, pp 107–162

    Chapter  Google Scholar 

  • Kumar R, Attah PK (1977) Structure of the organs of digestion and reproduction in the Tortoise Beetle, Aspidomorpha spp. (Chrysomelidae: Coleoptera). J Nat Hist 11(1):65–76

  • Maffei EMD, Fragoso DDB, Pompolo SDG, Serrão JE (2001) Morphological and cytogenetical studies on the female and male reproductive organs of Eriopis connexa Mulsant (Coleoptera, Polyphaga, Coccinellidae). Neth J Zool 51(4):483–496

    Article  Google Scholar 

  • Marzo L (2008) Studio sulla diversità anatomica della spermateca nei Coleotteri. Entomologica 41:13–76

    Google Scholar 

  • Melo ACA, Valle D, Machado EA, Salerno AP, Paiva-Silva GO, Silva NLCE, de Souza W, Masuda H (2000) Synthesis of vitellogenin by the follicle cells of Rhodnius prolixus. Insect Biochem Mol Biol 30:549–557

    Article  CAS  Google Scholar 

  • Metcalfe ME (1932) Memoirs: the structure and development of the reproductive system in the Coleoptera with notes on its homologies. J Cell Sci 2(297):49–129

    Article  Google Scholar 

  • Mohamed MI, Khaled AS, Fattah HMA, Hussein MA, Salem DA, Fawki S (2015) Ultrastructure and histopathological alteration in the ovaries of Callosobruchus maculatus (F.) (Coleoptera, Chrysomelidae) induced by the solar radiation. J Basic Appl Zool 68:19–32. https://doi.org/10.1016/j.jobaz.2014.12.004

    Article  Google Scholar 

  • Nation JL (1983) A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy. Stain Technol 58(6):347–351

    Article  CAS  Google Scholar 

  • Putman WL (1955) Bionomics of Stethorus punctillum Weise (Coleoptera: Coccinellidae) in Ontario. Can Entomol 87:9–33

    Article  Google Scholar 

  • Raikhel AS, Dhadialla TS (1992) Accumulation of yolk proteins in insect oocytes. Annu Rev Entomol 37:217–251. https://doi.org/10.1146/annurev.en.37.010192.001245

    Article  CAS  PubMed  Google Scholar 

  • Salazar K, Boucher S, Serrão JE (2017) Structure and ultrastructure of the ovary in the South American Veturius sinuatus (Eschscholtz) (Coleoptera, Passalidae). Arthropod Struct Dev 46(4):613–626. https://doi.org/10.1016/j.asd.2017.03.007

    Article  PubMed  Google Scholar 

  • Simões MV (2012) Male and female reproductive systems of Stolas conspersa (Germar) (Coleoptera, Chrysomelidae, Cassidinae). Rev Bras Entomol 56(1):19–22. https://doi.org/10.1590/S0085-56262012005000007

    Article  Google Scholar 

  • Stringer IAN (1988) The female reproductive system of Costelytra zealandica (White) (Coleoptera: Scarabaeidae: Melolonthinae). N Z J Zool 15(4):513–533

    Article  Google Scholar 

  • Świątek P, Górkiewicz T (2006) Microsporidia infect the Liophloeus lentus (Insecta, Colepotera) ovarioles, developing oocytes and eggs. Fol Biol 54(1–2):61–67. https://doi.org/10.3409/173491606777919120

  • Thakur ML (1999) Insect pest status of poplars in India. Indian For 125:866–872

    Google Scholar 

  • Tillesse V, Nef L, Charles J, Hopkin A, Augustin S (2007) Damaging poplar ınsects (ınternationally ımportant species). International Poplar Commission, FAO, Rome

    Google Scholar 

  • Túler AC, Silva Torres CS, Teixeira VW, Teixeira AA, Guedes CA, D’Assunção CG, Brayner FA, Alves LC (2018) Histology of the spermateca and stored sperm of Tenuisvalvae notata (Coleoptera: Coccinellidae). Physiol Entomol 43(3):180–187. https://doi.org/10.1111/phen.12242

    Article  Google Scholar 

  • Urban J (2006) Occurrence, bionomics and harmfulness of Chrysomela populi L. (Coleoptera; Chrysomelidae). J For Sci 52:255–284

    Article  Google Scholar 

  • Wan Nurul ‘Ain WMN, Nurul Wahida O, Yaakop S, Norefrina Shafinaz MN (2018) Morphology and histology of reproductive organ and first screening of Wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae). Serangga 23(2):183–193

    Google Scholar 

  • Wang XP, Zhou XM, Wang YY, Lei CL (2007) Internal reproductive system and diapausing morphology of the Brassica leaf beetle Phaedon brassicae Baly (Coleoptera: Chrysomelidae: Chrysomelinae). Coleopt Bull 61(3):457–462

    Article  Google Scholar 

  • Wheeler DE (2009) Reproduction, female. In: Vincent HR, Ring TC (eds) Encyclopedia of insects, 2nd edn. Academic, California, pp 183–201

    Google Scholar 

  • Wheeler DE (2009) Ovarioles. In: Vincent HR, Ring TC (eds) Encyclopedia of insects, 2nd edn. Academic, California, pp 743–744

    Chapter  Google Scholar 

  • Wigthman JA, Southgate BJ (1982) Egg morphology, host, and probable regions of origin of the bruchids (Coleoptera: Bruchidae) that infest stored pulses-an identification aid. New Zeal J Exp Agr 10:95–99

    Article  Google Scholar 

  • Zeki H, Toros S (1996) The effect of host on the adults of Chrysomela populi L. and Chrysomela tremulae F. (Col:Chrysomelidae). Bitki Kor Bült 36:25–38

    Google Scholar 

  • Zhang Z (2011) Animal biodiversity: an outline of higher-level classification and survey of taxonomic richness. Zootaxa 3148:1–237

    Article  Google Scholar 

  • Zhang SQ, Che LH, Li Y, Liang D, Pang H, Ślipiński A, Zhang P (2018) Evolutionary history of Coleoptera revealed by extensive sampling of genes and species. Nat Commun 9(1):1–11. https://doi.org/10.1038/s41467-017-02644-4

    Article  CAS  Google Scholar 

  • Zimowska G, Shirk PD, Silhacek DL, Shaaya E (1994) Yolk sphere formation is initiated in oocytes before development of patency in follicles of the moth Plodia interpunctella. Wilhelm Roux’s Arch Dev Biol 203:215–226

    Article  Google Scholar 

Download references

Acknowledgements

We thank Gazi University Academic Writing Center for revising the grammar of this article.

Author information

Authors and Affiliations

  1. Department of Biology, Science Faculty, Gazi University, Ankara, Turkey

    Nurcan Özyurt Koçakoğlu & Selami Candan

  2. Department of Plant Protection, Faculty of Agriculture, Bingöl University, Bingöl, Turkey

    Mustafa Güllü

Authors
  1. Nurcan Özyurt Koçakoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Selami Candan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mustafa Güllü
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nurcan Özyurt Koçakoğlu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest disclosed in this work.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Addıtıonal informatıon

This article was originally submitted by the authors in English and is first published here.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Özyurt Koçakoğlu, N., Candan, S. & Güllü, M. Morphology of the reproductive tract of females of leaf beetle Chrysomela populi (Chrysomelidae: Coleoptera). Biologia 76, 3257–3265 (2021). https://doi.org/10.1007/s11756-021-00796-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11756-021-00796-9

Keywords

Search

Navigation