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Application of luminescence for quality evaluation of in vitro regenerated strawberry plants

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Abstract

The parameters of chlorophyll a fluorescence induction were measured: Fv/Fm, Sc/Fm, Rfd and coefficient of Ld delayed luminescence decay kinetics, related with a course of primary photosynthesis reactions on leaves of strawberry plants, cultured in vitro by means of the micropropagation methods. Strawberry plants cv. Ananasowa from in vitro cultures in optimal condition show significantly higher values of luminescence parameters indicating better condition of plants of this variety in comparison with the variety Senga Sengana. After temperature lowering, however, these values were more reduced than for plants of Senga Sengana, which can be interpreted as higher susceptibility of this variety to chill. Addition of BAP caused disturbance of primary photosynthesis reactions rate, particularly in lower temperature. Auxin 2,4-D had no effect on the luminescence parameters in comparison with control cultures. Dehydration stress strongly diminished the values of measured parameters for Ananasowa variety what indicates the inhibition of primary photosynthesis reaction in leaves. The old culture of Senga Sengana variety showed higher tolerance on linuron in comparison with the new one.

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References

  • Burza W., Murkowski A., Malepszy S. 1994. Differences in the luminescence of regenerated cucumber plants caused by plant hormones in the medium. Gartenbauwissenschaft 59 (3): 105–108.

    CAS  Google Scholar 

  • Burza W., Murkowski A., Skórska E., 1996. Wpływ światła i chłodu na stan fizjologiczny roślin ogórka zregenerowanych w kulturze in vitro, Mat. Konf. “Zastosowanie kultur in vitro w fizjologii roślin”, ZFR PAN w Krakowie: 28.

  • Havaux, M., Lannoye, R. 1985. In vivo chlorophyll fluorescence and delayed light emission as rapid screening techniques for stress tolerance in crop plants. Z. Pflanzen. 95: 1–13.

    Google Scholar 

  • Hideg, E. Demeter, S. 1987. Effect of pH on the slow phase components of delayed luminescence in chloroplasts. Z. Naturforsch. 42c: 1121–1125.

    Google Scholar 

  • Krause, G.H., Somersalo, S. 1989. Fluorescence as a tool in photosynthesis research: application in studies of photoinhibition, cold acclimation and freezing stress. Phil. Trans. R. Soc. Lond. B323: 281–293.

    Google Scholar 

  • Krause, G.H., Weis, E. 1991. Chlorophyll fluorescence and photosynthesis: The Basics. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 313–349.

    Article  CAS  Google Scholar 

  • Lichtenthaler, H., Buschmann, C., Rinderle, U., Schmuck, G. 1986. Application of chlorophyll fluorescence in ecophysiology. Radiat. Environ. Biophys. 25: 297–308.

    Article  PubMed  CAS  Google Scholar 

  • Murashige T., Skoog F., 1962. A revised medium for rapid growth and bio-assayes with tobacco tissue cultures. Physiologia Plantarum. 15: 473–492.

    Article  CAS  Google Scholar 

  • Murkowski A., Burza W., Skórska E. 1995. Rapid estimation of physiological state of in vitro regenerated plants. Biotechnologia (Suppl.) 4(31): 100.

    Google Scholar 

  • Murkowski A., Burza W., Skórska E., 1997. The effect of light and chill on physiological state of cucumber plants regenerated in in vitro cultures. Acta Physiologiae Plantarum. 19(suppl.) (2): 228.

    Google Scholar 

  • Murkowski, A., Skórska, E. 1988. The application of the luminescence method to evaluation of wheat and rape susceptibility to certain herbicides. Hod. Roślin Aklim. Nasien. 32 (1/2): 171–174.

    Google Scholar 

  • Niemirowicz-Szczytt K., Malepszy S., 1980. Micropropagation from the meristems and young leaves of Fragaria. Bull. Acad. Polon. Sci. 28 (5): 335–340.

    CAS  Google Scholar 

  • Oquist G., Wass R., 1988. A portable, microprocessor operated instrument for measuring chlorophyll fluorescence kinetics in stress physiology. Physiol. Plant. 73: 211–217

    Article  Google Scholar 

  • Rutherford, A.W., Inoue, Y. 1984. Oscillation of delayed luminescence from PSII: recombination of S2QB-and S3QB-. FEBS Lett. 165 (2): 163–170.

    Article  CAS  Google Scholar 

  • Skórska, E., Murkowski A. 1988. Photosynthetic luminescence detection as the quick test of chilling resistance of cucumber plants. Hod. Roślin Aklim. Nasien. 32 (1/2): 285–289.

    Google Scholar 

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Authors and Affiliations

  1. Department of Physics, Agricultural University of Szczecin, ul. Pawła VI 3, 71-454, Szczecin

    Antoni Murkowski, Grzegorz Bartoszewski & Elżbieta Skórska

  2. Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, ul.Nowoursynowska 166, 02-766, Warszawa

    Grzegorz Bartoszewski

Authors
  1. Antoni Murkowski
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  2. Grzegorz Bartoszewski
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  3. Elżbieta Skórska
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Murkowski, A., Bartoszewski, G. & Skórska, E. Application of luminescence for quality evaluation of in vitro regenerated strawberry plants. Acta Physiol Plant 20, 465–470 (1998). https://doi.org/10.1007/s11738-998-0034-0

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  • DOI: https://doi.org/10.1007/s11738-998-0034-0

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