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A comparative study of different cytokinins on the formation of Rhododendron forrestii Balf. f. ex diels. axillary shoots in vitro

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Abstract

The effect of different concentrations and activities of cytokinins on the morphogenesis of regenerated Rhododendron forrestii Balf. f. ex Diels. shoots taken from nodal segments were tested. We evaluated zeatin, zeatin riboside, izopentyladenine, izopentyladenine riboside, kinetin, kinetin riboside, benzylaminopurine, benzylaminopurine riboside. The experimental results were evaluated by mathematical methods and regression analysis describing the effect of isoprenic and aromatic type of cytokinins. On the basis of this modelling, maximum axillary shoot production was attained with medium supplemented with 2.0 mg·l−1 izopentyladenine riboside, 2.0 mg·l−1 benzylaminopurine and 20 g·l−1 sucrose. Minimal axillary shoots were produced with kinetin and kinetin riboside.

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Abbreviations

BA:

6-benzylaminopurine

BAR:

6-benzylaminopurine riboside

2iP:

6-dimethylallylaminopurine

2iP-R:

6-dimethylallylaminopurine riboside

Z:

Zeatin

ZR:

Zeatin riboside

K:

Kinetin

KR:

Kinetin riboside

References

  • Anderson W.C. 1984. A revised tissue culture medium for shoot multiplication. of rhododendron. J. Amer. Soc. Hort. Sci., 109: 343–347.

    Google Scholar 

  • Amo-Marco J.B., Picazo I. 1994. Effect of growth regulators in vitro propagation of Ficus benjamina cv. Exotica. Biol. Plantarum. 36: 167–173.

    Article  Google Scholar 

  • Bojarczuk K. 1995. Regeneracja wybranych gatunków i odmian różaneczników z sadzonek pędowych i z kultur in vitro. Plantpress. Kraków: 1–112.

  • Cholvadova B., Korcova D., Vizarova G. 1996. Effects of different cytokinins type on growth of meristematic culture of Actinidia deliciosa (A. CHEV.). Biológia. 51: 81–82.

    Google Scholar 

  • Economou A. S., Read P.E. 1984. In vitro shoot proliferation of Minnesota deciduous Azaleas. Hort. Science. 19: 60–61.

    CAS  Google Scholar 

  • Fordham I., Stimart P. 1982. Axillary and adventitious shoot proliferation of Exbury azaleas in vitro. Hort Science. 17: 738–739.

    Google Scholar 

  • Heylen. C., Vendrig J. C. 1988. The influence of different cytokinins and auxins on flower neoformation in thin cell layers of Nicotiana tabacum L. Plant Cell Physiol. 29: 665–671.

    CAS  Google Scholar 

  • Kamenicka A., Rypak M. 1985. Možnosti rozmnožovania aktinídie v podmienkach in vitro. Záhradníctvo. 10: 463–464.

    Google Scholar 

  • Kamenicka A., Rypak M. 1989. Explantáty v rozmnožovaní drevín. Acta dendrobiologica. Veda. Bratislava. 1–160.

  • Marino G., Bertazza, G. 1990. Micropropagation of Actinidia deliciosa cos Hayward and Tomuri. Scientia Horticulture. 45: 65–74.

    Article  Google Scholar 

  • Mc Clelland M.T., Smith M.A.L. 1990. Vessel type, closure and explant orientation influence in vitro performance of five woody species. Hort Science. 25: 797–800.

    Google Scholar 

  • Monette P. L. 1986. Micropropagation of kiwifruit using nonaxenic shoot tips. Plant Cell Tissue Organ Culture. 6: 73–82.

    Article  CAS  Google Scholar 

  • Pruski K., Nowak J., Grainger G. 1990. Micropropagation of four cultivars of Saskatoon berry (Amelanchier alnifolia NUTT.). Plant Cell Tissue and Organ Culture. 21: 103–109.

    Article  CAS  Google Scholar 

  • Rahman M.A., Blake J. 1988. The effect of medium composition and culture conditions on in vitro rooting and ex vitro establishment of jockfruit (Artocarpus heterophyllus Lam.). Plant Cell Tissue and Organ Culture. 13: 189–200.

    Article  Google Scholar 

  • Strnad M., Hanus J., Peters W., Beck E. 1994. Orthotopolin-9-glucoside, a new aromatic cytokinin from Populus x canadensis cv. Robusta leaves. Phytochemistry. 37: 1059–1062.

    Article  CAS  Google Scholar 

  • Vizarova G., Masarovicova E. 1992. Seasonal changes of free endogenous cytokinins in the leaves of watered and non-watered oak seedlings. In: International symposium at the occasion of the 100-th anniversary of the Arboretum Mlynany foundation, ed. by P. Hrubik, Publ., Veda. Bratislava: 548–552.

  • Wassen M., Phipps J., Carbonneau R., Simmonds J. 1991. Plant growth substances in sugar maple (Acer saccharum Marsh) spring sap. Identification of cytokinins, abscisic acid and an indolic compound. J. Plant Physiol. 138: 489–493.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Forest Ecology, Slovak Academy of Sciences, Sturova 2, 960 53, Zvolen, Slovak Republic

    Aurelia Kamenicka & Jozef Valka

  2. Institute of Microbiology, Slovak Academy of Sciences, Stefanikova 3, 814 34, Bratislava, Slovak Republic

    Gabriela Vizarova

Authors
  1. Aurelia Kamenicka
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  2. Jozef Valka
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  3. Gabriela Vizarova
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Kamenicka, A., Valka, J. & Vizarova, G. A comparative study of different cytokinins on the formation of Rhododendron forrestii Balf. f. ex diels. axillary shoots in vitro . Acta Physiol Plant 20, 167–171 (1998). https://doi.org/10.1007/s11738-998-0009-1

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  • DOI: https://doi.org/10.1007/s11738-998-0009-1

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