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Water deficit-induced oxidative stress and differential response in antioxidant enzymes of tolerant and susceptible tea cultivars under field condition

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Abstract

Tea (Camellia sinensis (L) O. Kuntze) sustainable production is threatened by frequent drought. Tolerance to drought is a complex phenotypic response, which is controlled by the interactive properties of the plant’s genetic makeup, environment and its growing stage. The present investigation aimed to identify the differential responses of antioxidative enzymes, non-enzymatic antioxidants and status of osmolytes in tea plants under water deficit condition with respect to gradually depleting soil moisture content (SMC) and leaf relative water content (RWC). We examined the consequences of water deficit on the physiological characteristics and biochemical compounds of C. sinensis leaves. Our results indicated that the tolerant genotypes maintained favorable water relations, relatively higher photosynthetic pigments and reduced membrane injury by lowering hydrogen peroxide (H2O2) accumulation compared to susceptible cultivars. The relatively lower efficiency of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POX) in susceptible cultivars under subsequent water deficit stress make them more prone to magnified oxidative damage than the tolerant cultivars. Furthermore, a substantial enhancement in the accumulation of osmolytes (proline and glycine betaine) has a great contribution in tolerance during drought. These results provide the insight into the basic mechanisms necessary for induction of antioxidant defense system and accumulation of osmolytes under water deficit conditions.

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Acknowledgements

This work was supported by the Department of Biotechnology (DBT). We gratefully acknowledge the DBT for financial support, the Project No. BT/PR14818/NDB/51/217/2010. The Division of Botany is gratefully acknowledged for providing the DUS center to perform the experiments.

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Authors and Affiliations

  1. Division of Plant Physiology and Biotechnology, UPASI Tea Research Foundation Tea Research Institute, Nirar Dam P.O., Valparai, 642 127, Coimbatore, Tamil Nadu, India

    M. Nalina, R. Rajkumar, B. Radhakrishnan & K. N. Chandrashekara

  2. Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessarghatta Lake P.O., Bangalore, 560 089, Karnataka, India

    S. Saroja

  3. Molecular Biology Laboratory, Department of Genetics and Evolution, Federal University of Sao Carlos (UFSCar), Sao Carlos, SP, 13565905, Brazil

    M. Chakravarthi

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  1. M. Nalina
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Nalina, M., Saroja, S., Chakravarthi, M. et al. Water deficit-induced oxidative stress and differential response in antioxidant enzymes of tolerant and susceptible tea cultivars under field condition. Acta Physiol Plant 43, 10 (2021). https://doi.org/10.1007/s11738-020-03174-1

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