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Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses

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Abstract

It is known that somatic mutations arising during animal growth and ageing contribute to the development of neurodegenerative and other animal diseases. For plants, several studies showed that small-scale somatic DNA mutations accumulated during Arabidopsis life cycle. However, there is a lack of data on the influence of environmental stresses on somatic DNA mutagenesis in plants. In this study, we analyzed the effects of ultraviolet C (UV-C) irradiation, high soil salinity, and cadmium (CdI3) stresses on the level of small-scale somatic DNA mutations in Arabidopsis thaliana. The number of DNA mutations was examined in the Actin2 3′UTR (Actin-U1), ITS1-5.8rRNA-ITS2 (ITS), and ribulose-1,5-biphosphate carboxylase/oxygenase (rbcL) DNA regions. We found that somatic mutation levels considerably increased in CdI3-treated Arabidopsis plants, while the mutation levels declined in the UV-C- and NaCl-treated A. thaliana. Cadmium is a mutagen that is known to inhibit DNA repair processes. The detected stress-induced alterations in somatic DNA mutation levels were accompanied by markedly increased expression of base excision repair genes (AtARP, AtDME, AtDML2, AtDML3, AtMBD4, AtROS, AtUNG, and AtZDP), nucleotide excision repair genes (AtDDB1a, AtRad4, and AtRad23a), mismatch repair genes (AtMSH2, AtMSH3, and AtMSH7), and photoreactivation genes (AtUVR2, AtUVR3). Thus, the results demonstrated that UV-C, high soil salinity, and cadmium stresses influence both the level of DNA mutations and expression of DNA repair genes. Salt- and UV-induced activation of DNA repair genes could contribute to the stress-induced decrease in somatic mutation level.

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Acknowledgements

This work was supported by a Grant from the Russian Foundation for Basic Research (16-04-00839a).

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Authors and Affiliations

  1. Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia

    K. V. Kiselev, Z. V. Ogneva, A. S. Dubrovina, A. R. Suprun & A. P. Tyunin

  2. Department of Biochemistry, Biotechnology, and Microbiology, Far Eastern Federal University, Vladivostok, 690090, Russia

    K. V. Kiselev, Z. V. Ogneva & A. R. Suprun

Authors
  1. K. V. Kiselev
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  2. Z. V. Ogneva
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  3. A. S. Dubrovina
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  4. A. R. Suprun
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  5. A. P. Tyunin
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Correspondence to K. V. Kiselev.

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Communicated by M. Horbowicz.

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Kiselev, K.V., Ogneva, Z.V., Dubrovina, A.S. et al. Altered somatic mutation level and DNA repair gene expression in Arabidopsis thaliana exposed to ultraviolet C, salt, and cadmium stresses. Acta Physiol Plant 40, 21 (2018). https://doi.org/10.1007/s11738-017-2600-9

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  • DOI: https://doi.org/10.1007/s11738-017-2600-9

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