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Physiological and antioxidant enzyme gene expression analysis reveals the improved tolerance to drought stress of the somatic hybrid offspring of Brassica napus and Sinapis alba at vegetative stage

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Abstract

Drought is a major constraint of agriculture development. The intergeneric somatic hybrids between Brassica napus and Sinapis alba were created by electrofusion to obtain materials with enhanced drought tolerance. The drought tolerance of B. napus cv. Yangyou 6 (Y6) and one offspring line (W146) of the somatic hybrids was evaluated by morphological observation. Physiological parameters were determined in this study. Moreover, the activities of a few antioxidant enzymes and the transcript level of the antioxidant enzyme encoding genes were analyzed by qPCR. W146 and Y6 showed apparent wilting after drought stress for 7 days. However, Y6 wilted more severely than W146. The result of the physiological analysis showed that the relative electronic conductivity and malondialdehyde content of Y6 were higher than that of W146. The relative water content, net photosynthesis rate, proline content, and the activities of superoxide dismutase (SOD) and peroxidase in W146 were higher than that in Y6 after drought stress for 12 days. The DNA and nitrotetrazolium blue chloride staining analysis revealed less accumulation of O2 ·− and H2O2 in W146 than that in Y6 after drought stress. Moreover, the transcript level of some antioxidant enzyme encoding genes, such as Cu/ZnSOD, MnSOD, ascorbate peroxidase, glutathione reductase, and glutathione peroxidase in W146, was higher than that in Y6 under drought stress. Results revealed that line W146 showed more drought stress tolerance than Y6 because line W146 could reduce oxidative damage by efficient antioxidant systems.

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbate

AsA–GSH:

Ascorbate–glutathione

DAB:

Diaminobenzidine

DHA:

Dehydroascorbate

DHAR:

Dehydroascorbate reductase

Fer:

Ferritin

GPX:

Glutathione peroxidase

GR:

Glutathione reductase

GSSG:

Glutathione oxidized

MDA:

Malondialdehyde

MDHA:

Monodehydroascorbate

MDHAR:

Monodehydroascorbate reductase

NBT:

Nitrotetrazolium blue chloride

Pn:

Net photosynthesis rate

POD:

Peroxidase

Pro:

Proline

PVP:

Polyvinyl pyrrolidone

REC:

Relative electronic conductivity

ROS:

Reactive oxygen species

RWC:

Relative water content

SOD:

Superoxide dismutase

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grants 31330057, 31501335), the Jiangsu Province Science Foundation (Grants BK20150441, 15KJB180019), China Postdoctoral Science Foundation (2015M570482), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Yangzhou University for Excellent Talent Support Program.

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Authors and Affiliations

  1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Lingjun Xia, Liuqing Yang, Nianli Sun, Jian Li, Yujie Fang & Youping Wang

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  1. Lingjun Xia
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  2. Liuqing Yang
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  3. Nianli Sun
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  4. Jian Li
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  5. Yujie Fang
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Correspondence to Yujie Fang or Youping Wang.

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Communicated by W Zhou.

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Xia, L., Yang, L., Sun, N. et al. Physiological and antioxidant enzyme gene expression analysis reveals the improved tolerance to drought stress of the somatic hybrid offspring of Brassica napus and Sinapis alba at vegetative stage. Acta Physiol Plant 38, 88 (2016). https://doi.org/10.1007/s11738-016-2111-0

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