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Mycorrhizal colonization and seed treatment with salicylic acid to improve physiological traits and tolerance of flaxseed (Linum usitatissimum L.) plants grown under drought stress

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Abstract

Application of mycorrhizal fungi and salicylic acid (SA) has been found very effective in reducing the adverse affects of drought. Our research examined interaction between seed treatment with 250 µM SA and two mycorrhizal fungi [Funeliformis mosseae (FM) and Rhizophagus intraradices (RI)] under different irrigation levels [100, 70, and 40 % of field capacity (FC)] on photosynthetic pigments, relative water content (RWC), proline content, leaf area (LA), dry weight (DW), phosphorus content, mycorrhizal colonization percentage (MCP) and mycorrhizal dependency (MD) in flaxseed plants during 160 days in 2010–2011. Drought stress reduced LA, plant P, DW, and RWC, but increased soil P, proline content, MCP, and MD, but had no marked effects on plant pigments. In mycorrhizal plants, chlorophyll, LA, RWC, MD, and MCP increased under drought and non-stress condition and RI was more effective than FM on these traits especially under severe drought. Salicylic acid application had positive effect only on MCP and P in the plant under 100 and 70 % FC, while reduced MD, MCP, and plant P at 40 % FC. Moreover, SA application increased LA, plant P, and RWC of non-mycorrhizal plants, but decreased these traits in mycorrhizal plants. In addition, there was antagonistic action between AM fungi and SA. SA may reduce positive effects of mycorrhizal fungi on flaxseed performances under both stress and non-stress conditions. The results suggested that AM inoculation may increase flaxseed growth under both stress and non-stress conditions, while SA application may reduce growth. Moreover, when SA application combines with AM inoculation flaxseed growth may decline suggesting that there may not be synergic effect between these treatments on flaxseed growth.

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Abbreviations

AM:

Arbuscular mycorrhizal

SA:

Salicylic acid

FM:

Funeliformis mosseae

RI:

Rhizophagus intraradices

FC:

Field capacity

RWC:

Relative water content

LA:

Leaf area

DW:

Dry weight

MCP:

Mycorrhizal colonization percentage

MD:

Mycorrhizal dependency

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Acknowledgments

This manuscript was supported by a grant from the Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology.

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  1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Aida Ansari, Jamshid Razmjoo & Hassan Karimmojeni

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  1. Aida Ansari
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  2. Jamshid Razmjoo
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  3. Hassan Karimmojeni
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Correspondence to Hassan Karimmojeni.

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Ansari, A., Razmjoo, J. & Karimmojeni, H. Mycorrhizal colonization and seed treatment with salicylic acid to improve physiological traits and tolerance of flaxseed (Linum usitatissimum L.) plants grown under drought stress. Acta Physiol Plant 38, 34 (2016). https://doi.org/10.1007/s11738-015-2054-x

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