Skip to main content
SpringerLink
Log in

Effect of several medium factors on polysaccharide and alkaloid accumulation in protocorm-like bodies of Dendrobium candidum during bioreactor culture

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Polysaccharides and alkaloids are the most important bioactive compounds found in Dendrobium candidum. For mass production of polysaccharide and alkaloids from protocorm-like bodies (PLBs), this study optimized medium condition by investigating several factors affecting D. candidum PLB biomass and bioactive compound accumulation in bioreactors. During PLB bioreactor culture, sucrose is a suitable carbon source at an optimal concentration of 30 g l−1 for polysaccharide and alkaloid production. High production of polysaccharides and alkaloids was determined in salt strengths of 1/2×, 3/4×, and full strength of the MS medium. Phosphate and ammonium/nitrate ratio in normal half-strength MS medium had to be adjusted, and 1.8 mmol phosphate and 30 mmol of NO3 − as the sole nitrogen source were suitable. Production of bioactive compounds by PLB bioreactor culture is a feasible and efficient method, and polysaccharide and alkaloid yields can be improved by modifying the culture medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

BA:

Benzyl aminopurine

CW:

Coconut water

DW:

Dry weight

FW:

Fresh weight

MS:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

PLBs:

Protocorm-like bodies

vvm:

Air volume/culture medium volume per min

References

  • Biao L, Wang BC, Tang K, Liang YL, Wang J, Wei JM (2006) A simple and convenient approach for isolating RNA from highly viscous plant tissue rich in polysaccharides. Colloid Surf B 49:101–105

    Article  Google Scholar 

  • Bush L, Wilkinson H, Schardl C (1997) Bioprotective alkaloids of grass-fungal endophyte symbioses. Plant Physiol 114:1–7

    PubMed Central  CAS  PubMed  Google Scholar 

  • Cha XQ, Luo JP (2004) Nutrient requirement for growth and polysaccharide accumulation in liquid cultures of protocorm-like dodies of Dendrobium huoshanene. J Hefei Univ Technol 27:53–57

    Google Scholar 

  • Cha XQ, Luo JP, Jiang ST (2005) Studies on the synthesis of active polysaccharides in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Food Sci 26:41–44

    Google Scholar 

  • Chen X, Guo S (2001) Advances in the research of constituents and pharmacology of Dendrobium. Nat Prod Res Dev 13:70–75

    CAS  Google Scholar 

  • Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520

    Article  CAS  Google Scholar 

  • Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:4708–4716

    Article  CAS  PubMed  Google Scholar 

  • Cui HY, Baque MA, Lee EJ, Paek KY (2013) Scale-up of adventitious root cultures of Echinacea angustifolia in a pilot-scale bioreactor for the production of biomass and caffeic acid derivatives. Plant Biotechnol Rep 7:297–308

    Article  Google Scholar 

  • Cui HY, Murthy HN, Moh SH, Cui YY, Lee EJ, Paek KY (2014) Production of biomass and bioactive compounds in protocorm cultures of Dendrobium candidum Wall ex Lindl. using balloon type bubble bioreactors. Ind Crop Prod 53:28–33

    Article  CAS  Google Scholar 

  • Dan Y, Meng ZX, Guo SX (2012) Effects of forty strains of orchidaceae mycorrhizal fungi on growth of protocorms and plantlets of Dendrobium candidum and D. nobile. Afr J Microbiol Res 6:34–39

    Google Scholar 

  • Dubios M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  Google Scholar 

  • Gao R, Wu SQ, Piao XC, Park SY, Lian ML (2014) Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor systems. Acta Physiol Plant 36:117–124

    Article  CAS  Google Scholar 

  • He TG, Yang LT, Li YR, Wang CQ, Su J (2007) Effect of sucrose on growth of protocorms of Dendrobium candidum and accumulation of polysaccharide. J Anhui Agric Sci 35:3817–3819

    Google Scholar 

  • Huang T, Gao W, Wang J (2010) Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer. Acta Physiol Plant 32:765–772

    Article  Google Scholar 

  • Jiang ST, Wei M, Luo JP (2006) Effect of phosphate on cell growth and polysaccharide production by suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Chin J Biotechnol 22:613–617

    Article  CAS  Google Scholar 

  • Leung PC, Cheng KF (2008) Good agricultural practice (GAP)—Does it ensure a perfect supply of medicinal herbs for research and drug development? Int J Appl Res Nat Prod 1:1–8

    Google Scholar 

  • Li M, Hirata Y, Xu G, Niwa M (1990) Determination of polysaccharide contents in the drugs of Dendrobium. Chin Tradit Herb Drugs 21:10–12

    Google Scholar 

  • Misic D, Siler B, Skoric M, Djurickovic MS, Zivkovic JN, Jovanovic V, Giba Z (2013) Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. Process Biochem 48:1587–1591

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nagella P, Murthy HN (2011) Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tiss Org Cult 104:119–124

    Article  CAS  Google Scholar 

  • Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell Dev Biol Plant 37:149–157

    Article  CAS  Google Scholar 

  • Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large-scale production of horticultural and medicinal plants. Plant Cell Tiss Org Cult 81:287–300

    Article  Google Scholar 

  • Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss Org Cult 63:67–72

    Article  Google Scholar 

  • Shiau YJ, Nalawade SM, Hsia CN, Mulabagal V, Tsay HS (2005) In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segments. In Vitro Cell Dev Biol Plant 41:666–670

    Article  Google Scholar 

  • Shim KM, Hahn EJ, Jeon WK, Paek KY (2010) Accumulation of cell biomass, anthraquinones, phenolics, and flavonoids as affected by auxin, cytokinin, and medium salt strength in cell suspension culture of Morinda citrifolia. Kor J Hortic Sci Technol 28:288–294

    CAS  Google Scholar 

  • Sivakumar G, Kim SJ, Hahn EJ, Paek KY (2005) Optimizing environmental factors for large-scale multiplication of chrysanthemum (Chrysanthemum grandiflorum) in balloon-type bioreactor culture. In Vitro Cell Dev Biol Plant 41:822–825

    Article  CAS  Google Scholar 

  • Thorpe T, Meier D (1972) Starch metabolism, respiration, and shoot formation in tobacco callus cultures. Physiol Plant 27:365–369

    Article  CAS  Google Scholar 

  • Wei XX, Su J, He TG (2008) Effects of several factors on protocorm growth and polysaccharide accumulation in suspension cultures of protocorm of Dendrobium candidum. Guangxi Agr Sci 39:601–606

    Google Scholar 

  • Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199

    Article  CAS  Google Scholar 

  • Wu RZ, Chakrabarty D, Hahn EJ, Paek KY (2007) Micropropagation of an endangered jewel orchid (Anoectochilus formosanus) using bioreactor system. Hortic Environ Biotechnol 48:376–380

    CAS  Google Scholar 

  • Yang JF, Piao XC, Sun D, Lian ML (2010) Production of protocorm-like bodies with bioreactor and regeneration in vitro of Oncidium ‘Sugar Sweet’. Sci Hortic 125:712–717

    Article  CAS  Google Scholar 

  • Yin SS, Gao WY, Liang YY, Wang J, Liu H, Wei CL, Zuo BM (2013a) Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla. Acta Physiol Plant 35:1579–1585

    Article  CAS  Google Scholar 

  • Yin SS, Liang YY, Gao WY, Wang J, Jing SS, Zhang Y, Liu H (2013b) Influence of medium salt strength and nitrogen source on biomass and metabolite accumulation in adventitious root cultures of Pseudostellaria heterophylla. Acta Physiol Plant 35:2623–2628

    Article  CAS  Google Scholar 

  • Yoon YJ, Murthy HN, Hahn EJ, Paek KY (2007) Biomass production of Anoectochilus formosanus Hayata in a bioreactor system. J Plant Biol 50:573–576

    Article  Google Scholar 

  • Yu KW, Gao WY, Hahn EJ, Paek KY (2001) Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer). J Plant Biol 44:179–184

    Article  CAS  Google Scholar 

  • Zeng SJ, Wang J, Wu KL, da Silva JAT, Zhang JX, Duan J (2013) In vitro propagation of Paphiopedilum hangianum Perner and Gruss. Sci Hortic 151:147–156

    Article  CAS  Google Scholar 

  • Zhao P, Wang W, Feng FS, Wu F, Yang ZQ, Wang WJ (2007) High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall Ex Lindl. Plant Cell Tiss Org Cult 90:131–139

    Article  Google Scholar 

  • Zhao P, Wu F, Feng FS, Wang WJ (2008) Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cell Dev Biol Plant 44:178–185

    Article  CAS  Google Scholar 

  • Zheng Y, Jiang W, Silva EN, Mao L, Hannaway DB, Lu H (2012) Optimization of shade condition and harvest time for Dendrobium candidum plants based on leaf gas exchange, alkaloids and polysaccharides contents. Plant Omics 5:253–260

    CAS  Google Scholar 

  • Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Process Biochem 23:671–675

    Article  Google Scholar 

  • Zhong JJ, Zhu QX (1995) Effect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of Panax notoginseng. Appl Biochem Biotechnol 55:241–247

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Science Foundation of China (31160079).

Author information

Authors and Affiliations

  1. Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Ministry of Education, Yanbian University, Park Road 977, Yanji, 133002, Jilin, China

    Fan Yang, Nan-Nan Wei, Ri Gao, Xuan-Chun Piao & Mei-Lan Lian

Authors
  1. Fan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nan-Nan Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ri Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xuan-Chun Piao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mei-Lan Lian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mei-Lan Lian.

Additional information

Communicated by M. Capuana.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, F., Wei, NN., Gao, R. et al. Effect of several medium factors on polysaccharide and alkaloid accumulation in protocorm-like bodies of Dendrobium candidum during bioreactor culture. Acta Physiol Plant 37, 94 (2015). https://doi.org/10.1007/s11738-015-1843-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-015-1843-6

Keywords

Search

Navigation