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Nitric oxide is involved in salicylic acid-induced flowering of Lemna aequinoctialis Welw.

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Abstract

Salicylic acid (SA) is a well-known inducer of flowering in Lemna under both non-inductive and inductive photoperiod conditions. However, the underlying mechanism is not well understood. Herein, we report for the first time that nitric oxide (NO) is partially involved in SA-induced flowering in L. aequinoctialis (Syn. L. paucicostata Hegelm.). Our results demonstrated that SA-induced flowering is significantly reduced by exogenous application of NO scavengers; 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and methylene blue, nitric oxide synthase inhibitors; N-ω-nitro-l-arginine and N-ω-nitro-l-arginine-methyl ester hydrochloride, and nitrate reductase inhibitor; sodium tungstate in two strains of Lemna viz. 6746 and LP6. Altogether our present findings shed a light on the new role of NO in SA-induced flowering and open interesting directions that need further investigation.

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Acknowledgments

AK acknowledges the awards of Junior Research Fellowship and Senior Research Fellowship by the Council of Scientific and Industrial Research (CSIR), New Delhi. We are grateful to Professor J.P. Khurana, Department of Plant Molecular Biology, University of Delhi South Campus, Delhi, India for providing aseptic cultures of L. aequinoctialis Welw., strains 6746 and LP6. AK thanks Dr. Hridayesh Prakash, University of Hyderabad, India for critical reading of the manuscript. The research work presented in this paper was also supported by R&D Miscellaneous Grants given to SBB from the University of Delhi.

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  1. Ashima Khurana

    Present address: Botany Department, Zakir Husain Delhi College, University of Delhi, New Delhi, 110002, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, New Delhi, 110007, India

    Ashima Khurana & Shashi B. Babbar

  2. Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India

    Rahul Kumar

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  1. Ashima Khurana
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Correspondence to Ashima Khurana.

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Communicated by A. Gniazdowska-Piekarska.

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Khurana, A., Kumar, R. & Babbar, S.B. Nitric oxide is involved in salicylic acid-induced flowering of Lemna aequinoctialis Welw.. Acta Physiol Plant 36, 2827–2833 (2014). https://doi.org/10.1007/s11738-014-1600-2

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