Skip to main content

Advertisement

SpringerLink
Log in

Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicum L.) in response to soil water deficits under field conditions

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

A field experiment was conducted to evaluate the response of ten sesame genotypes to different levels of soil water in terms of contents of proline, soluble carbohydrates, carotenoids, and activities of catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX). Plants were grown under three irrigation levels, including irrigation at 55 % (control), 75, and 85 % depletion of soil available water. Field test plots were a two-way factorial arranged in a randomized complete block design with three replications. Under control level of irrigation, the most and the least grain yields were achieved for genotypes Ultan (2,519 kg/ha) and Isfahan1 (1,311 kg/ha), respectively. Grain yield was decreased in some genotypes under 75 % and in all genotypes under 85 % depletion of available water. Based on percentage reduction in grain yield under both 75 and 85 % depletion of soil available water, Isfahan4, Borazjan, Isfahan1, Ahvaz, Ardestan, and Shiraz were recognized as relatively tolerant and Ultan, Shahreza, Kal, and Markazi were identified as relatively sensitive to water stress. The activities of antioxidant enzymes and the contents of carotenoids, proline, and soluble carbohydrates in leaves were increased in most genotypes under stress conditions, and the magnitudes of the increases were greater in the tolerant than in the sensitive genotypes. The results of this experiment showed that the stress-induced increase of antioxidant enzymes and the contents of the compatible solutes in leaves were related to the tolerance of sesame genotypes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abedi T, Pakniyat H (2010) Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Plant Breed 46:27–34

    CAS  Google Scholar 

  • Alizadeh A (2004) Soil, water and plant relationship, 4th edn. Emam Reza University Press, Mashhad (ISBN. 964-6582-57-50)

    Google Scholar 

  • Allen RG, Pereira LS, Raes D, Smith M (2000) FAO irrigation and drainage paper. Crop Evapotranspiration (Guidel Comput Crop Water Requir) 56:1–326

    Google Scholar 

  • Andrew JS, Moreau H, Kuntz M, Pagny G, Lin C, Tanksley S, McCarthy J (2008) An investigation of carotenoid biosynthesis in Coffea canephora and Coffea arabica. Plant Physiol 165:1087–1106

    Article  Google Scholar 

  • Bartoli CG, Simontacchi M, Tambussi E, Beltrano J, Montaldi E, Puntarulo S (1999) Drought and watering-dependent oxidative stress: effect on antioxidant content in Triticum aestivum L. leaves. J Exp Bot 50:375–383

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39(1):205–207

    Article  CAS  Google Scholar 

  • Boydak E, Karaaslan D, Simsek M, Gercak S, Kirnak H, Kaspa Y, Ozturk I (2007) Effect of irrigation methods and irrigation interval on yield and some yield components of sesame growing in semi-arid area. J Agron 6(3):439–443

    Article  Google Scholar 

  • Brar GS, Kar S, Singh NT (1990) Photosynthetic response of wheat to soil water deficits in the tropics. J Agron Crop Sci 164:343–348

    Article  Google Scholar 

  • Chance B, Maehly AC (1955) Assay of catalases and peroxidase. Method Enzymol 2:764–775

    Article  Google Scholar 

  • Demmig-Adams B, Adams WW (1996) Chlorophyll and carotenoid composition in leaves of Euonymus kiautschovicus acclimated to different degrees of light stress in the field. Aust J Plant Physiol 23:649–659

    Article  CAS  Google Scholar 

  • Deng X, Hu ZA, Wang HX, Wen XG, Kuang TY (2003) A comparison of photosynthetic apparatus of the detached leaves of the resurrection plant Boea hygrometrica with its non-tolerant relative Chirita heterotricha in response to dehydration and rehydration. Plant Sci 165:851–861

    Article  CAS  Google Scholar 

  • Fazeli F, Ghorbanli M, Niknam V (2007) Effect of drought on biomass, protein content, lipid peroxidation and anti-oxidant enzymes in two sesame cultivars. Biol Plant 51:98–103

    Article  CAS  Google Scholar 

  • Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars. 1. Grain yields responses. Aust J Agric Res 29:897–912

    Article  Google Scholar 

  • Gao J (2006) Experimental techniques of plant physiology. Higher Education Press, Beijing (In Chinese)

    Google Scholar 

  • Ghorbanli M, Ebrahimzadeh H, Sharifi M (2004) Effects of NaCl and mycorrhizal fungi on antioxidative enzymes in soybean. Biol Plant 48:575–581

    Article  CAS  Google Scholar 

  • Glombitza C, Dubuis PH, Thulke O (2004) Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism. Plant Mol Biol 51:1–19

    Google Scholar 

  • Hassanzadeh M, Ebadi M, Panahyan-e-Kivi M, Jamaati-e-Somarin SH, Saeidi M, Zabihi-e-Mahmoodabad R (2009) Evaluation of drought stress on relative water content and chlorophyll content of sesame (Sesamum indicum L.) genotypes at early flowering stage. Res J Environ Sci 3(3):345–350

    Article  CAS  Google Scholar 

  • Havaux M, Tardy F (1999) Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress. Aust J Plant Physiol 26:569–578

    Article  CAS  Google Scholar 

  • Hendry GAF, Price AH (1993) Stress indications: chlorophylls and carotenoids. In: Hendry GAF, Grime JP (eds) Methods in comparative plant ecology, 1st edn. Chapman and Hall, London, pp 148–152

    Chapter  Google Scholar 

  • Herzog V, Fahimi H (1973) Determination of the activity of peroxidase. Anal Biochem 55:554–562

    Article  CAS  PubMed  Google Scholar 

  • Irigoyen JJ, Emerich DW, Sanchez-Diaz M (1992) Water stress induce changes in concentration of proline and total soluble sugar in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60

    Article  CAS  Google Scholar 

  • Jaleel CA, Manivannan P, Wahid A, Farooq M, Somasundaram R, Panneerselvam R (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. Int J Agri Biol 11:100–105

    Google Scholar 

  • Karaaslan D, Boydak E, Gercek S, Simsek M (2007) Influence of irrigation intervals and row spacing on some yield components of sesame grown in Harran region. Asian J Plant Sci 6:623–662

    Article  Google Scholar 

  • Kholova J, Hash CT, Kocova M, Vadez V (2011) Does a terminal drought tolerance QTL contribute to differences in ROS scavenging enzymes and photosynthetic pigments in pearl millet exposed to drought? Environ Exp Bot 71:99–106

    Article  CAS  Google Scholar 

  • Kim KS, Park SH, Jenks MA (2006) Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit. J Plant Physiol 164:1134–1143

    Article  PubMed  Google Scholar 

  • Kreps JA, Wu YJ, Chang HS (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mensah JK, Obadoni BO, Eruotor PG, Onome-Irieguna F (2006) Simulated flooding and drought effects on germination, growth, and yield parameters of sesame (Sesamum indicum L.). Afr J Biotech 5(13):1249–1253

    CAS  Google Scholar 

  • Mitra J (2001) Genetics and genetic improvement of drought resistance in crop plants. Curr Sci 80:758–762

    CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9(10):490–498

    Article  CAS  PubMed  Google Scholar 

  • Munne-Bosch S, Alegre L (2000) Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants. Planta 207:925–931

    Article  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Nazarli H, Faraji F, Zardashti MR (2011) Effect of drought stress and polymer on osmotic adjustment and photosynthetic pigments of sunflower. Cercetări Agronomice în Moldova XLIV(1(145)):35–41

    Google Scholar 

  • Noctor G, Foyer CH (1998) Ascorbate and glutathione. Keeping active oxygen under control. Ann Rev Plant Physiol Mol Biol 49:249–279

    Article  CAS  Google Scholar 

  • Parida AK, Dagaonkar VS, Phalak MS, Umalkar GV, Aurangabadkar LP (2007) Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery. Plant Biotechnol Rep 1:37–48

    Article  Google Scholar 

  • Pastori GM, Trippi VS (1992) Antioxidative protection in a drought-resistant maize strain during leaf senescence. J Plant Physiol 87:227–231

    Article  Google Scholar 

  • Pinhero RG, Pao MV, Palyath G, Murr DP, Fletcher RA (2000) Changes in the activities of antioxidant enzymes and their relationship to genetic and paclobutrazol induced chilling tolerance of maize seedlings. J Plant Physiol 114:695–704

    Google Scholar 

  • Ramachandra Reddy A, Viswanatha Chaitanya K, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202

    Article  PubMed  Google Scholar 

  • Rudoplh AS, Crowe JH, Crowe LM (1986) Effects of three stabilizing agents-proline, betaine, and trehalose on membrane phospholipids. Arch Biochem Biophys 245:134–143

    Article  Google Scholar 

  • Saeidi A, Tohidi-Nejad E, Ebrahimi F, Mohammadi-Nejad G, Shirzadi MH (2012) Investigation of water stress on yield and some yield components of sesame genotypes (Sesamum indicum L.) in Jiroft Region. J Appl Sci Res 8(1):243–246

    Google Scholar 

  • Selote DS, Khanna-Chopra R (2004) Drought-induced spikelet sterility is associated with an inefficient antioxidant defense in rice panicles. Plant Physiol 121:462–471

    Article  CAS  Google Scholar 

  • Shao HB, Liang ZS, Shao MA (2003) Roles of ABA signal transduction during higher plant seed maturation and germination. Forestry Stud China 5(4):42–51

    Google Scholar 

  • Shao HB, Liang ZS, Shao MA (2005) Impacts of PEG-6000 pretreatment for barley (Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism. Colloids Surf B Biointerfaces 41(2–3):73–77

    CAS  Google Scholar 

  • Sharma P, Dubey RS (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul 46:209–221

    Article  CAS  Google Scholar 

  • Tantawy MM, Ouda SA, Khalil FA (2007) Irrigation optimization for different sesame varieties grown under water stress conditions. J Applied Sci Res 3:7–12

    Google Scholar 

  • Terzi R, Kadioglu A (2006) Drought stress tolerance and the antioxidant enzyme system in Ctenanthe setosa. Acta Biol Cracov Bot 48(2):89–96

    Google Scholar 

  • Vasconcelos ACF, Zhang XZ, Ervin EH, Kiehl JD (2009) Enzymatic antioxidant responses to Biostimulants in maize and soybean subjected to drought. Scientia Agricola 66(3):395–402

    Article  Google Scholar 

  • Wang JR, Li SX, Li KI (2001) Effect of water limited deficit stress during different growth stages on leaf enzymes of winter wheat. Acta Bot Borel Occident Sin 21(1):47–52

    CAS  Google Scholar 

  • Yang D, Shen X, Zhao T, Ma X (2001) Achievement of drought-injury physiology in maize. Crops (In Chinese) 5:1–4

    Google Scholar 

  • Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71

    Article  CAS  PubMed  Google Scholar 

  • Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6(5):441–445

    Article  CAS  PubMed  Google Scholar 

  • Zhu JH, Shi HZ, Lee BH (2004) An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. PNAS 101(26):9873–9878

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Isfahan University of Technology.

Author information

Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Isfahan University of Technology, Isfahan, Iran

    Azam Kadkhodaie, Morteza Zahedi & Jamshid Razmjoo

  2. School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    Mohammad Pessarakli

Authors
  1. Azam Kadkhodaie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Morteza Zahedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jamshid Razmjoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Pessarakli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammad Pessarakli.

Additional information

Communicated by G. Bartosz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kadkhodaie, A., Zahedi, M., Razmjoo, J. et al. Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicum L.) in response to soil water deficits under field conditions. Acta Physiol Plant 36, 641–650 (2014). https://doi.org/10.1007/s11738-013-1442-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-013-1442-3

Keywords

Search

Navigation