Abstract
Some technological and physiological aspects were analysed during seed storage of holm oak (Quercus ilex L.), a typical Mediterranean recalcitrant species. Considering the mild dormancy and desiccation sensitivity of these seeds, the influence of the storage environment on viability was examined. Acorns were maintained at low temperature and at high moisture content either inside thin polyethylene bags or mixed with peat in a bin. Storage in polyethylene bags, if compared with peat treatment, maintained optimal seed quality parameters. The effects of the two treatments on some physiological parameters were analysed during 1-year seed storage. Acorns stored in a bin with peat started to germinate early and exhibited a more rapid seed deterioration caused by metabolism-linked oxidative damage. The latter phenomenon was linked to a higher respiration and H2O2 level, induction of catalase activity, as well as lowered glutathione pool and ATP content. In addition, a more oxidized redox poise was observed. On the contrary, the plastic film of polyethylene, limiting gaseous exchanges, maintained acorn metabolic activity at low levels and allowed the accumulation of ethylene inside the storage atmosphere. These factors, inducing a dormant-like state, could have played a crucial role in prolonging seed storage.
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References
Akimoto T, Cho S, Yoshida H, Furuta H, Esashi Y (2004) Involvement of acetaldehyde in seed deterioration of some recalcitrant woody species through the acceleration of aerobic respiration. Plant Cell Physiol 45:201–210
Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res 14:93–107
Baker MA, Cerniglia GJ, Zaman A (1990) Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190:360–365
Berjak P, Pammenter NW (2001) Seed recalcitrance—current perspectives. S Afr J Bot 67:79–89
Berjak P, Pammenter NW (2007) From Avicennia to Zizania: seed recalcitrance in perspective. Ann Bot 101:213–228
Berjak P, Farrant JM, Pammenter NW (1989) The basis of recalcitrant seed behaviour. In: Taylorson RB (ed) Recent advances in the development and germination of seeds. Plenum Press, New York, pp 89–108
Bewley JD, Black M (1994) Seeds. Physiology of development and germination, 2nd edn. Plenum Press, New York, pp 140–143
Bonner FT (1996) Responses to drying of recalcitrant seeds of Quercus nigra L. Ann Bot 78:181–187
Bonner FT (2003) Collection and care of acorns. A practical guide for seed collectors and nursery managers-version 1.1. Starkville
Bonner FT, Vozzo JA (1987) Seed biology and technology of Quercus. USDA, Forest service, General Technical Report, New Orleans, GTR SO-66
Chao C, Lin T (1996) Content of adenylate nucleotides and energy charge in the early stage of germination of orthodox and recalcitrant seeds. Bot Bull Acad Sin 37:229–237
Charef M, Yousfi M, Saidi M, Stocker P (2008) Determination of the fatty acid composition of acorn (Quercus), Pistacia lentiscus seeds growing in Algeria. J Am Oil Chem Soc 85:921–924
Chirkova TV (1988) Plant adaptation to hypoxia and anoxia. Leningrad State University Press, Leningrad, ISBN 5-288-00072-7, pp 1–245
Connor KF, Sowa S (2002) Recalcitrant behaviour of temperate forest tree seeds: storage, biochemistry and physiology. In: Proceedings of the 11th biennial southern silvicultural research conference. USDA Forest Service, Southern Research Station: Asheville, General Technical Report GTR-SRS-48, pp 47–50
Corbineau F, Gay-Mathieu C, Vinel D, Côme D (2002) Decrease in sunflower (Helianthus annuus) seed viability caused by temperature as related to energy metabolism, membrane damage and lipid composition. Physiol Plant 116:489–496
Esashi Y, Ooshima Y, Michiharu A, Akiko K, Satoh S (1986) CO2-enrichment of C2H4 production in tissues of imbibed cocklebur seeds. Austr J Plant Physiol 13:417–429
Farnsworth E (2000) The ecology and physiology of viviparous and recalcitrant seeds. Annu Rev Ecol Syst 31:107–138
Farrant JM, Pammenter NW, Berjak P (1988) Recalcitrance—a current assessment. Seed Sci Tech 16:155–166
Farrant JM, Bailly C, Leymarie J, Hamman B, Côme D, Corbineau F (2004) Wheat seedlings as a model to understand desiccation tolerance and sensitivity. Physiol Plant 120:563–574
Finch-Savage WE, Clay HA (1994) Evidence that ethylene, light and abscisic acid interact to inhibit germination in the recalcitrant seeds of Quercus robur L. J Exp Bot 45:1295–1299
Finch-Savage WE, Grange RI, Hendry GAF, Atherton NM (1993) Embryo water status and loss of viability during desiccation in the recalcitrant species Quercus robur L. In: Côme D, Corbineau F (eds) Proceedings of the Fourth International Workshop on Seeds: basic and applied aspects of seed biology. ASFIS, Paris, pp 723–730
Finch-Savage WE, Blake PS, Clay HA (1996) Desiccation stress in recalcitrant Quercus robur L. seeds results in lipid peroxidation and increased synthesis of jasmonates and abscisic acid. J Exp Bot 47:661–667
Greggains V, Finch-Savage WE, Quick WP, Atherton NM (2000) Metabolism-induced free radical activity does not contribute significantly to loss of viability in moist-stored recalcitrant seeds of contrasting species. New Phytol 148:267–276
Halliwell B, Gutteridge JMC (1999) How organisms deal with oxidative protein damage. In: Halliwell B, Gutteridge JMC (eds) Free Radicals in Biology and Medicine, 3rd edn. Oxford University Press, Oxford, pp 320–321
Hanhijärvi AM, Fagerstedt K (1995) Comparison of carbohydrate utilisation and energy charge in the yellow flag iris (Iris pseudacorus) and garden iris (Iris germanica) under anoxia. Physiol Plant 93:493–497
Hendry GAF, Finch-Savage WE, Thorpe PC, Atherton NM, Buckland SM, Nilsson KA, Seel WE (1992) Free radical processes and loss of seed viability during desiccation in the recalcitrant species Quercus robur L. New Phytol 122:273–279
Iakovoglou V, Misra MK, Hall RB, Knapp AD (2010) Alterations of seed variables under storage in nitrous oxide (N2O) atmospheres for two recalcitrant Quercus species. Scand J For Res 25:24–30
International seed testing association (2010) Chapter 9 Determination of Moisture Content. In: International rules for seed testing, IX 1–IX 20, adopted at the Ordinary Meeting 2007, Iguaçu Falls, Brazil
Kępczyński J, Kępczyńska E (1997) Ethylene in seed dormancy and germination. Physiol Plant 101:720–726
Kibinza S, Vinel D, Côme D, Bailly C, Corbineau F (2006) Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiol Plant 128:496–506
King MW, Roberts EH (1979) The storage of recalcitrant seeds: achievements and possible approaches. In: International Board for Plant Genetic Resources, IBPGR Secretariat, FAO, Rome, Italy
Kranner I, Birtić S, Anderson KM, Pritchard HW (2006) Glutathione half-cell reduction potential: a universal stress marker and modulator of programmed cell death? Free Rad Biol Med 40:2155–2165
Kranner I, Minibayeva FV, Beckett RP, Seal CE (2010) What is stress? Concepts, definitions and applications in seed science. New Phytol 188:655–673
Leprince O, Vertucci CW, Hendry GAF, Atherton NM (1995) The expression of desiccation-induced damage in orthodox seeds is a function of oxygen and temperature. Physiol Plant 94:233–240
Monteleone I, Cartarasa M, Belletti P (2001) Aspetti sementieri e vivaistici del leccio. Sherwood, Foreste ed alberi oggi 7:35–39
Oracz K, El-Maarouf Bouteau H, Farrant JM, Cooper K, Belghazi M, Job C, Job D, Corbineau F, Bailly C (2007) ROS production and protein oxidation as a novel mechanism of seed dormancy alleviation. Plant J 50:452–465
Pammenter NW, Berjak P (1999) A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Sci Res 9:13–37
Pammenter NW, Berjak P, Farrant JM, Smith MT, Ross G (1994) Why do stored hydrated recalcitrant seeds die? Seed Sci Res 4:187–191
Panochit J, Wasuwanich P, Hellum AK (1984) Collection, germination and storage of Shorea siamensis Miq. seeds. Embryon 1:1–13
Pasquini S, Braidot E, Petrussa E, Vianello A (2011) Effect of different storage conditions in recalcitrant seeds of holm oak (Quercus ilex L.) during germination. Seed Sci Technol 39:165–177
Petrussa E, Bertolini A, Casolo V, Krajnáková J, Macrì F, Vianello A (2009) Mitochondrial bioenergetics linked to the manifestation of programmed cell death during somatic embryogenesis of Abies alba. Planta 231:93–107
Pukacka S, Gawronska H (2002) Changes in abscisic acid levels in embryo axes of Norway maple and sycamore seeds during maturation and dehydration. Acta Physiol Plant 24:149–155
Ratajczak E, Pukacka S (2006) Changes in the ascorbate–glutathione system during storage of recalcitrant seeds of Acer saccharinum L. Acta Soc Bot Pol 1:23–27
Rawyler A, Pavelic D, Gianinazzi C, Oberson J, Braendle R (1999) Membrane lipid integrity relies on a threshold of ATP production rate in potato cell cultures submitted to anoxia. Plant Physiol 120:293–300
Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1:499–514
Szalai G, Kellös T, Galiba G, Kocsy G (2009) Glutathione as an antioxidant and regulatory molecule in plants under abiotic stress conditions. J Plant Growth Regul 28:66–80
Valio IFM, De L, Ferreira Z (1992) Germination of seeds of Myrciaria cauliflora (Mart.) Berg. (Myrthaceae). Rev Bras Fisiol Veg 4:95–98
Walters C, Pammenter NW, Berjak P, Crane J (2001) Desiccation damage, accelerated ageing and respiration in desiccation tolerant and sensitive seeds. Seed Sci Res 11:135–148
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This work was supported by the University of Udine and by the NCFB (National Centre for the Study and the Conservation of the Forestry Biodiversity), Peri (Italy), in the mainframe of the studies on the forestry biodiversity and its conservation.
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Communicated by M. Horbowicz.
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Pasquini, S., Mizzau, M., Petrussa, E. et al. Seed storage in polyethylene bags of a recalcitrant species (Quercus ilex): analysis of some bio-energetic and oxidative parameters. Acta Physiol Plant 34, 1963–1974 (2012). https://doi.org/10.1007/s11738-012-0996-9
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DOI: https://doi.org/10.1007/s11738-012-0996-9