Abstract
Shoot branching is considered a key factor that influences plant architecture and productivity. In the present study, the open reading frame encoding lateral shoot-inducing factor, named JLIF, was cloned from Jatropha curcas L. JLIF encoded a 214 amino acid polypeptide and contained a strongly conserved QALGGH motif in zinc-finger domain which is the typical motif of the SUP protein family. Sequence and phylogenetic analysis indicated that JLIF had very high similarity (97.67%) to the LIF gene in petunia, but showed no significant similarity to other known SUP proteins except for the conserved motifs. Taken together, we presumed JLIF was the ortholog of LIF protein, and both appeared to belong to a novel subfamily of the SUP proteins. Semi-quantitative RT-PCR analysis revealed JLIF was abundant in stems and petioles, weakly expressed in leaves and seeds, but absent in roots. Subsequently, the JLIF gene was introduced into tobacco under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. PCR analysis and Southern blot hybridization were performed to confirm if JLIF was stably integrated into the tobacco genome. GUS activity analysis and RT-PCR revealed the location of the overexpressed JLIF. Overexpression of JLIF in transgenic tobaccos resulted in reduced plants height and shortened internodes in vitro.
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Acknowledgments
This work was supported by the grants from [‘‘Eleventh Five Years’’ Key Project of Chinese Ministry of Science and Technology] (no. 2007BAD50B01-02-04 and no. 2007BAD50B05), Hundred Talents Project of Southwest Jiaotong University.
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Communicated by J.-H. Liu.
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Zong, H., Wang, S., Ouyang, C. et al. Cloning, characterization and overexpression of the complete open reading frame of the lateral shoot-inducing factor from Jatropha Curcas . Acta Physiol Plant 34, 317–326 (2012). https://doi.org/10.1007/s11738-011-0831-8
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DOI: https://doi.org/10.1007/s11738-011-0831-8