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The effect of exogenous and endogenous phytohormones on the in vitro development of gametophyte and sporophyte in Asplenium nidus L.

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Abstract

The fern Asplenium nidus L. is in great demand as an ornamental plant. The aim of this work was to investigate the influence of phytohormones in promoting a gametophytic and sporophytic growth in homogenized sporophytes tissue. Exogenous application of 0.5 and 5 μM N 6-benzyladenine, 0.05 and 0.5 μM indole-3-acetic acid (IAA), and 0.3 and 3 μM gibberellic acid (GA3) favoured sporophyte regeneration, whereas gametophyte regeneration took place when plant material was cultured in a hormone-free liquid MS medium. The endogenous contents of the auxin IAA, the cytokinins trans-zeatin, trans-zeatin riboside, dihydrozeatin, dihydrozeatin riboside, isopentenyladenine and isopentenyladenosine, and the gibberellins GA1, GA3, GA4, GA7, GA9 and GA20 in growing gametophytes and sporophytes were evaluated. Similar levels of the auxin and cytokinins and qualitative differences in the gibberellins were found between both generations.

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Abbreviations

BAP:

N6-benzyladenine

CKs:

Cytokinins

DHZ:

Dihydrozeatin

DHZR:

Dihydrozeatin riboside

DW:

Dry weight

FW:

Fresh weight

GAs:

Gibberellins

HPLC-MS/MS:

High-performance liquid chromatography-tandem mass spectrometry

IAA:

Indole-3-acetic acid

iP:

Isopentenyladenine

iPR:

Isopentenyladenosine

MS:

Murashige and Skoog nutrient medium (1962)

tZ:

Trans-zeatin

tZR:

Trans-zeatin riboside

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Acknowledgments

The authors thank to Science and Technologies Ministry for supporting this research through the project MEC2006 CGL 06578, and also FYCIT Program Severo Ochoa.

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Authors and Affiliations

  1. Lab of Plant Physiology, Department of BOS, c) Catedrático R Uría s/n, 33071, Oviedo, Spain

    V. Menéndez, Y. Abul & H. Fernández

  2. Biotechnical Faculty, Centre for Plant Biotechnology and Breeding, Jamnikarjeva 101, 1111, Ljubljana, Slovenia

    B. Bohanec

  3. Mass Spectrometry Unit, Edificio Ramón y Cajal, Campus Rabanales, Cordova, Spain

    F. Lafont

Authors
  1. V. Menéndez
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  2. Y. Abul
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  3. B. Bohanec
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  4. F. Lafont
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  5. H. Fernández
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Corresponding author

Correspondence to H. Fernández.

Additional information

Communicated by P. K. Nagar.

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Menéndez, V., Abul, Y., Bohanec, B. et al. The effect of exogenous and endogenous phytohormones on the in vitro development of gametophyte and sporophyte in Asplenium nidus L.. Acta Physiol Plant 33, 2493–2500 (2011). https://doi.org/10.1007/s11738-011-0794-9

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  • DOI: https://doi.org/10.1007/s11738-011-0794-9

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