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Identification of cDNAs for jasmonic acid-responsive genes in Polygonum minus roots by suppression subtractive hybridization

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Abstract

Elicitation, the plant-based biotechnology approach that utilizes the ability of plant roots to absorb and secrete a vast variety of bioactive compounds, was studied on Polygonum minus using jasmonic acid (JA) as an elicitor. To understand the overall molecular responses of P. minus roots to JA induction, a subtracted cDNA library was constructed using the suppression subtractive hybridization (SSH) method. From a total of 1,344 randomly selected colonies, 190 clones were shown to be differentially expressed using Reverse Northern hybridization. BLAST analysis revealed that clones were similar to genes associated with the biosynthesis of aromatic compounds through the oxylipin pathway, such as alcohol dehydrogenase and lipoxygenase. Putative clones involved in the shikimate pathway, including S-adenosyl-l-methionine synthetase and S-adenosyl-l-homocysteine hydrolase, were identified with predicted roles in phenylpropanoids’ biosynthesis. Genes responding to abiotic stress unique to JA elicitation, such as ELI3-1, glutathione S-transferase and peroxidase 1, were also identified. The kelch-repeat containing F-box family protein, a possible transcription factor in response to JA elicitation was also found. The results of the RT-PCR showed that the eight selected clones were strongly up-regulated, except for lipoxygenase, which showed a slightly higher expression of the transcript levels in response to the JA elicitation.

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Acknowledgments

This work was supported by Ministry of Higher Education of Malaysia through FRGS grant (UKM-RB-FRGS0001-2006) awarded to Ismanizan Ismail. Miss Gor Mian Chee is a recipient of the National Science Foundation (NSF) fellowship granted by Ministry of Science, Technology and Innovation of Malaysia.

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Authors and Affiliations

  1. School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Mian Chee Gor, Ismanizan Ismail, Zamri Zainal, Normah Mohd Noor, Roohaida Othman & Zeti Azura Mohamed Hussein

  2. Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Ismanizan Ismail, Zamri Zainal, Normah Mohd Noor, Roohaida Othman & Zeti Azura Mohamed Hussein

  3. School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Wan Aida Wan Mustapha

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  1. Mian Chee Gor
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Correspondence to Ismanizan Ismail.

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Communicated by S. Abe.

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Gor, M.C., Ismail, I., Mustapha, W.A.W. et al. Identification of cDNAs for jasmonic acid-responsive genes in Polygonum minus roots by suppression subtractive hybridization. Acta Physiol Plant 33, 283–294 (2011). https://doi.org/10.1007/s11738-010-0546-2

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