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Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP

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Abstract

CMS is induced by the coordinated expression of certain mitochondrial and nuclear genes in flower development. Mitochondrial genes regulate manifestation of CMS, whereas nuclear genes regulate fertility phenotype and thus affect negatively. In this article, the buds of newly bred Ogura CMS of non-heading Chinese cabbage and its maintainer line as plant materials, genes differentially expressed transcripts were analyzed by cDNA-AFLP. Seventeen differently expressed genes were found in new Ogura CMS and nine genes in maintainer line. These genes were involved in energy metabolism, signal transduction, flower development, stress-related metabolism, transcription, etc. Expression patterns of three genes encoding BrCAM6, BrANK, BrTUB3 were verified by qRT-PCR in different organs and various stage flower buds of CMS and its maintainer line. The results revealed that two genes related to signal transduction, BrCAM6 and BrANK, were highly expressed in stamens and microspores of CMS than in maintainer line. As believed, these two genes involved in signal transduction of male sterile in CMS line. In comparison, BrTUB3 gene was accumulated in stamens and was expressed in significantly lower level in CMS line than in maintainer line. It expressed significantly lower in CMS than maintainer line after tetrad stage. This expression profile suggests that BrTUB3 played an important role in the development of the pollen, and may be closely related to male sterility.

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Abbreviations

CMS:

Cytoplasmic male sterility

cDNA-AFLP:

cDNA-amplified fragment length polymorphism

qRT-PCR:

quantitative Real-time RT-PCR

BrCAM6 :

Calcium-ion-binding 6

BrANK :

Ankyrin repeat family protein

BrTUB3 :

Tubulin alpha-3

GAPDH :

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

We wish to thank Dr. Zengcui Zhang (North Dakota State University, USA) and Dr. Chowda Reddy (Southern Crop Protection and Food Research Centre, Canada) for critical reading the manuscript. This research was supported by the national natural science foundation of China(30671420) and Scientific research Innovations Program of Graduate students in General higher learning institutions of Jiangsu province in 2008 (CX08B_154Z).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 1# Weigang, Nanjing, Jiangsu, 210095, People’s Republic of China

    Changwei Zhang, Li Qi, Xilin Hou, Gongjun Shi & Jingyi Zhang

  2. Key Laboratory of Southern Vegetable Crop Genetic Improvement, College of Horticulture, Nanjing Agricultural University, Ministry of Agriculture, 1# Weigang, Nanjing, Jiangsu, 210095, People’s Republic of China

    Xilin Hou

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  1. Changwei Zhang
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  2. Li Qi
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  3. Xilin Hou
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  4. Gongjun Shi
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  5. Jingyi Zhang
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Corresponding author

Correspondence to Xilin Hou.

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Communicated by L. A. Kleczkowski.

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Zhang, C., Qi, L., Hou, X. et al. Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP. Acta Physiol Plant 32, 781–787 (2010). https://doi.org/10.1007/s11738-010-0463-4

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