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Phenolic compounds accumulation in Hypericum ternum propagated in vitro and during plant development acclimatization

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Abstract

The phenolic compound content of Hypericum ternum was investigated after micropropagation establishment and during acclimatization over the phenological development of the plant. Plantlets cultured in vitro on full Murashige and Skoog medium without growth regulators displayed higher phenolic compound yields, were acclimatized, and field grown. Production of total phenolic compounds as well as hyperoside, chlorogenic acid, quercitrin, guaijaverin, isoquercitrin, and uliginosin B were quantified at vegetative, flowering and fructification stages, and different plant organs (roots, stems, leaves and reproductive parts) showing that reproductive parts at flowering stage and the leaves at fructification stage were the main repository site of secondary metabolites, except for uliginosin B. The stems were the least accumulative organ, while the roots accumulated only hyperoside and uliginosin B. Moreover, the accumulation of most of the flavonoids and uliginosin B in acclimatized plants surpassed the levels found in the wild plant, warranting further research with the species.

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Authors and Affiliations

  1. Faculdade de Farmácia, UFRGS, Av. Ipiranga, 2752, Porto Alegre, 90610-000, Brazil

    Amanda Valle Pinhatti, Jéssica de Matos Nunes, Gilsane Lino von Poser & Sandra Beatriz Rech

  2. Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM/UFRGS), Porto Alegre, Brazil

    Natasha Maurmann

  3. Departamento de Plantas Forrageiras e Agrometeorologia, Faculdade de Agronomia, UFRGS, Av. Bento Gonçalves, 7712, Caixa Postal 15100, Porto Alegre, Brazil

    Luís Mauro Gonçalves Rosa

Authors
  1. Amanda Valle Pinhatti
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  2. Jéssica de Matos Nunes
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  3. Natasha Maurmann
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  4. Luís Mauro Gonçalves Rosa
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  5. Gilsane Lino von Poser
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  6. Sandra Beatriz Rech
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Correspondence to Sandra Beatriz Rech.

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Communicated by S. Lewak.

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Pinhatti, A.V., de Matos Nunes, J., Maurmann, N. et al. Phenolic compounds accumulation in Hypericum ternum propagated in vitro and during plant development acclimatization. Acta Physiol Plant 32, 675–681 (2010). https://doi.org/10.1007/s11738-009-0446-5

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  • DOI: https://doi.org/10.1007/s11738-009-0446-5

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