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Evidence for the presence of non-receptor protein tyrosine kinases in algal cells

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Abstract

Two orders of green alga (Cladophorales and Charales) were investigated for the presence of protein tyrosine kinase activity. Proteins of 70 and 85 kDa were found to be tyrosine phosphorylated in Cladophora fracta, with an additional phosphorylated band evident at the 120-kDa region in Chara vulgaris, suggestive of the presence of putative tyrosine kinase activity in these algal species. A 70-kDa protein was immunoprecipitated from both species using a polyclonal antibody against non-receptor protein tyrosine kinase Syk. The protein was found to be phosphorylated on tyrosine, which was prevented upon pretreatment of algal cells with piceatannol. The extent of phosphorylation directly correlated with algal growth, suggesting a link between Syk kinase activity and growth signaling. These observations supported the presence of Syk-like kinase in the green algal species, which could have critical role in the algal growth and development.

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Acknowledgments

The author thankfully acknowledges grants received from the Department of Science and Technology, Government of India, under the DST Women Scientist Scheme (WOS-A), and the University Grants Commission, under the UGC Part-time Research Associateship Scheme.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Anjali Dash

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  1. Anjali Dash
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Correspondence to Anjali Dash.

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Communicated by H. Janska.

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Dash, A. Evidence for the presence of non-receptor protein tyrosine kinases in algal cells. Acta Physiol Plant 32, 177–182 (2010). https://doi.org/10.1007/s11738-009-0395-z

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  • DOI: https://doi.org/10.1007/s11738-009-0395-z

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