Skip to main content
SpringerLink
Log in

NaCl induced cross-acclimation to UV-B radiation in four Barley (Hordeum vulgare L.) cultivars

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The effect of pre-treatment with 200 mM NaCl on the response of four barley cultivars (Hordeum vulgare L. cv. Bülbül-89, Kalaycı-97, Tarm-92 and Tokak-157/37) to UV-B radiation was investigated. Salt stress as well as UV-B irradiation led to a decrease of the total chlorophyll (chl) content in all cultivars, except in Kalaycı-97. While carotenoids are almost not affected by NaCl treatment, UV-B irradiation caused an increase by 5–20% of carotenoid content of all cultivars. UV-B induced damages of photosynthetic apparatus were estimated by the rate of photosynthetic electron transport measured by chl fluorescence and the rate of oxygen evolution, the latter being more affected. Pre-treatment with NaCl alleviated harmful effect of UV-B irradiation on F v/F m and ETR, but not on oxygen evolution. UV-B-induced and UV-B-absorbing compounds with absorption at 300 and 438 nm increased as a result of UV-B treatment. The level of stress marker proline increased considerably as a result of NaCl treatment, while UV-B irradiation resulted in a pronounced increase of the level of H2O2. MDA enhanced in the seedlings subjected to salt and UV-B stress. Established cross-acclimation to UV-B as a result of salt treatment could be due to the increased free proline and the level of UV-B absorbing compounds in barley seedlings subjected to NaCl.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

Chl:

Chlorophyll

ETR:

Electron transport rate

Fo and Fm:

Initial and maximum fluorescence yield

MDA:

Malondialdehyde

PPFD:

Photosynthetic photon flux density

PSII:

Photosystem II

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

Tris:

Tris[hydroxymethyl]-aminomethane

References

  • Allen DJ, McKee IF, Farage PK, Baker NR (1997) Analysis of the limitation to CO2 assimilation on the exposure of leaves of two Brassica napus cultivars to UV-B. Plant Cell Environ 20:633–640

    Article  CAS  Google Scholar 

  • Allen DJ, Nogues S, Baker NR (1998) Ozone depletion and increased UV-B radiation: is there a real threat to photosynthesis? J Exp Bot 49:1775–1788

    CAS  Google Scholar 

  • Barnes PW, Maggard S, Holma SR, Vergara B (1993) Intraspecific variation in sensitivity to UV-B radiation in rice. Crop Sci 33:1041–1046

    Article  Google Scholar 

  • Barsig M, Malz R (2000) Fine structure, carbohydrates and photosynthetic pigments of sugar maize leaves under UV-B radiation. Environ Exp Bot 43:121–130

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bharti AK, Khurana JP (1997) Mutants of Arabidopsis as tools to understand the regulation of phenylpropanoid pathway and UV-B protection mechanisms. Photochem Photobiol 65:765–776

    Article  CAS  PubMed  Google Scholar 

  • Bieza K, Lois R (2001) An Arabidopsis mutant tolerant to lethal ultraviolet-B levels shows constitutively elevated accumulation of flavonoids and other phenolics. Plant Physiol 126:1105–1115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Caldwell MM (1971) Solar UV irradiation and the growth and development of higher plants. In: Giese AC (ed) Photophysiology. Academic Press, New York, pp 131–177

    Chapter  Google Scholar 

  • Carletti P, Masi A, Wonisch A, Grill D, Tausz M, Ferretti M (2003) Changes in antioxidant and pigment pool dimensions in UV-B irradiated maize seedlings. Environ Exp Bot 50:149–157

    Article  CAS  Google Scholar 

  • Chris A, Zeeshan M, Abraham G, Prasad SM (2006) Proline accumulation in Cylindrospermum sp. Environ Exp Bot 57:154–159

    Article  CAS  Google Scholar 

  • Correia CM, Areal ELV, Torres-Pereira MS, Torres-Pereira JMG (1999) Intraspesific variation in sensitivity to ultraviolet-B radiation in maize grown under field conditions. II. Physiological and biochemical aspects. Field Crops Res 62:97–105

    Article  Google Scholar 

  • Fedina I, Grigorova I, Georgieva K (2003) Response of barley seedlings to UV-B radiation as affected by NaCl. J Plant Physiol 160:205–208

    Article  CAS  PubMed  Google Scholar 

  • Fedina I, Velitchkova M, Georgieva K, Grigorova I (2005) UV-B-induced compounds as affected by proline and NaCl in Hordeum vulgare L. cv. Alfa. Environ Exp Bot 54:182–191

    Article  CAS  Google Scholar 

  • Fedina I, Georgieva K, Velitchkova M, Grigorova I (2006) Effect of pretreatment of barley seedlings with different salts on the level of UV-B induced and UV-B absorbing compounds. Environ Exp Bot 56:225–230

    Article  CAS  Google Scholar 

  • Greenway H, Munns R (1980) Mechanism of salt tolerance in nonhalophytes. Annu Rev Plant Physiol 31:149–190

    Article  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  PubMed  Google Scholar 

  • Hideg E, Vass I (1996) UV-B induced free radical production in plant leaves and isolated thylacoid membranes. Plant Sci 115:251–260

    Article  CAS  Google Scholar 

  • Hidema J, Kumagai T (2006) Sensitivity of rice to ultraviolet-B radiation. Ann Bot 97:933–942

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Larkum AWD, Karge M, Reifarth HJ, Post A, Renger G (2001) Effect of monochromatic UV-B radiation on electron transfer reactions of photosystem II. Photosynth Res 68:49–60

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic membranes. Methods Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Lois R, Buchanan BB (1994) Severe sensitivity to ultraviolet radiation in an Arabidopsis mutant deficient in flavonoid accumulation. II. Mechanisms of UV-resistance in Arabidopsis. Planta 194:504–509

    Article  Google Scholar 

  • Mirecki RM, Teramura AH (1984) Effects of ultraviolet-B irradiance on soybean V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion. Plant Physiol 74:475–480

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Misra AN, Sahu SM, Misra M (1995) Soil salinity-induced changes in pigment and protein contents in cotyledons and leaves of Indian mustard (Brassica juncea Coss.). Acta Physiol Plant 17:375–380

    CAS  Google Scholar 

  • Ries G, Heller W, Puchta H, Sandermann H, Seidlitzz HK, Hohn B (2000) Elevated UV-B radiation reduces genome stability in plants. Nature 406:98–101

    Article  CAS  PubMed  Google Scholar 

  • Ryan KG, Swinny EE, Markham KR, Winefield C (2002) Flavonoid gene expression and UV photoprotection in transgenic and mutant petunia leaves. Phytochemistry 59:23–32

    Article  CAS  PubMed  Google Scholar 

  • Sato T, Kumagai T (1993) Cultivar differences in resistance to the inhibitory effects of near UV radiation among Asian ecotype and Japanese lowland and upland cultivars of rice (Oriza sativa L.). Jpn J Breed 43:61–68

    Article  Google Scholar 

  • Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10:51–62

    Article  CAS  PubMed  Google Scholar 

  • Soheila A, Mackerness H (2000) Plant responses to ultraviolet-B (UV-B: 280–320 nm) stress: What are the key regulators? Plant Growth Regul 32:27–39

    Article  Google Scholar 

  • Strid A (1993) Increased expression of defence genes in Pisum sativum after exposure to supplementary ultraviolet-B radiation. Plant Cell Physiol 34:949–953

    CAS  Google Scholar 

  • Strid A, Porra RJ (1992) Alterations in pigment content in leaves of Pisum sativum after exposure to supplementary UV-B. Plant Cell Physiol 33:1015–1023

    CAS  Google Scholar 

  • Takeuchi Y, Fukumoto R, Kasahra H, Sakaki T, Kitao M (1995) Peroxidation of lipids and growth inhibition induced by UV-B irradiation. Plant Cell Rep 14:566–570

    Article  CAS  PubMed  Google Scholar 

  • Teramura AH, Sullivan JH (1994) Effect of UV-B radiation on photosynthesis and growth of terrestrial plants. Photosynth Res 39:463–473

    Article  CAS  PubMed  Google Scholar 

  • Teramura AH, Ziska K, Sztein AE (1991) Changes in growth and photosynthetic capacity of rice with increase UV-B radiation. Physiol Plant 83:373–380

    Article  CAS  Google Scholar 

  • Tevini M, Braun J, Fieser G (1991) The protective function of epidermal layer of rice seedlings against ultraviolet-B radiation. Photochem Photobiol 53:329–333

    Article  CAS  Google Scholar 

  • Willekens H, Van Camp W, Van Montagu M, Inze D, Langebartels C, Sandermann H (1994) Ozone, sulfur dioxide, and ultraviolet-B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L. Plant Physiol 106:1007–1014

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wilson MI, Greenberg BM (1993) Protection of the D1 photosystem II reaction center protein from degradation in ultraviolet radiation following adaptation of Brassica napus L. to growth in ultraviolet-B. Photochem Photobiol 57:556–5563

    Article  CAS  Google Scholar 

  • Yamamoto HY, Bassi R (1996) Carotenoids: localization and function. In: Ort RR, Yokum FC (eds) Oxygenic photosynthesis. The light reactions, Kluwer, Dordrecht, pp 539–563

    Google Scholar 

Download references

Acknowledgments

This research was supported by The Scientific and Technological Researched Council of Turkey, Project number, TBAG-U/135 and Bulgarian Academy of Sciences.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Hüsnü Çakırlar & Nuran Çiçek

  2. Academic Metodi Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street, Building 21, Sofia, 1113, Bulgaria

    Ivanka Fedina & Katya Georgieva

  3. Department of Biology, Faculty of Art and Sciences, Sakarya University, Esentepe Campus, 54187, Sakarya, Turkey

    Ali Doğru

  4. Institute of Biophysics, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street, Building 21, Sofia, 1113, Bulgaria

    Maya Velitchkova

Authors
  1. Hüsnü Çakırlar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nuran Çiçek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ivanka Fedina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Katya Georgieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ali Doğru
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maya Velitchkova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nuran Çiçek.

Additional information

Communicated by F. Corbineau.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Çakırlar, H., Çiçek, N., Fedina, I. et al. NaCl induced cross-acclimation to UV-B radiation in four Barley (Hordeum vulgare L.) cultivars. Acta Physiol Plant 30, 561–567 (2008). https://doi.org/10.1007/s11738-008-0155-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-008-0155-5

Keywords

Search

Navigation