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Oxidative events during in vitro regeneration of sunflower

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Abstract

The changes in the activity of some antioxidant enzymes and endogenous H2O2 level in zygotic sunflower embryos during organogenesis and somatic embryogenesis were monitored. Pathways of regeneration were induced on media differing with sucrose concentration 87 mmol dm−3 for shoot [shoot induction medium (SIM) medium] and 350 mmol dm−3 [embryo induction medium (EIM) medium] for somatic embryo induction. Water potential of the explants cultured on SIM increased, while the embryos maintained on EIM showed middle water deficit stress. The pattern of superoxide dismutase (SOD) isoforms was similar in organogenic and embryogenic culture; however, the intensity of MnSOD bands was higher on SIM than on EIM. Differences in catalase activity were observed: high activity on SIM predominated, whereas on EIM it was reduced. The activity of guaiacol peroxidase in the explants producing shoots and somatic embryos differed at the beginning of culture, but became comparable at the time of shoot and somatic embryo formation (day 5). H2O2 content was unchanged in organogenic culture, but on EIM it increased on day 1 followed by significant decrease. The results indicate that sugar concentration per se, or via induction of different developmental pathways influences the activity of antioxidant enzymes and also H2O2 level in cultured sunflower embryos.

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Abbreviations

AOS:

active oxygen species

BA:

6-benzyladenine

CAT:

catalase (EC 1.11.1.6)

DDT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

EIM:

embryo induction medium

MES:

2-[morpholino]ethanesulfonic acid

NBT:

nitro blue tetrazolium

PAGE:

polyacrylamide gel electrophoresis

POD:

guaiacol peroxidase (EC 1.11.1.7)

SIM:

shoot induction medium

SOD:

superoxide dismutase (EC 1.15.1.1)

TEMED:

N, N, N′, N- tetramethylethylenediamine

Tricine:

N-tris[hydroxymethyl]methylglycine

Tris:

tris(hydroxymethyl)aminomethane

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Authors and Affiliations

  1. Department of Plant Cytology and Embryology, Jagiellonian University, ul. Grodzka 52, 31-044, Cracow, Poland

    Robert Konieczny & Monika Tuleja

  2. Department of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239, Cracow, Poland

    Marta Libik, Ewa Niewiadomska & Zbigniew Miszalski

  3. Pedagogical Academy, Institute of Biology, ul. Podbrzezie 3, 31-054, Cracow, Poland

    Zbigniew Miszalski

Authors
  1. Robert Konieczny
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  2. Marta Libik
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  3. Monika Tuleja
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  4. Ewa Niewiadomska
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  5. Zbigniew Miszalski
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Corresponding author

Correspondence to Robert Konieczny.

Additional information

Communicated by J. Rybczynski.

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Konieczny, R., Libik, M., Tuleja, M. et al. Oxidative events during in vitro regeneration of sunflower. Acta Physiol Plant 30, 71–79 (2008). https://doi.org/10.1007/s11738-007-0092-8

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  • DOI: https://doi.org/10.1007/s11738-007-0092-8

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