Skip to main content
SpringerLink
Log in

The effect of Fe-EDDHA and of ascorbic acid on in vitro rooting of the peach rootstock GF-677 explants

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The objective of this research was to study the effect of the chelated form of the iron salt of ethylenediamine di-o-hydroxyphenylacetic acid (Fe-EDDHA) (6% Fe) on in vitro rooting of the rootstock GF-677. The iron salt of ethylenediamine tetraacetic acid (Fe-EDTA) (12% Fe) of the MS basic medium was replaced by Fe-EDDHA, which was applied in three concentrations: 93.5, 187.0 and 280 mg l−1 (5.6, 11.2 and 16.8 mg l−1 Fe, respectively). For each treatment of Fe-EDDHA, the effect of ascorbic acid added in four concentrations (0, 0.1, 1.0 and 10 mg l−1) was studied. After 4 weeks of culture, the explants growing on the medium with 280 mg l−1 Fe-EDDHA gave the best rooting results. Regarding ascorbic acid, no clear stimulating effect on rooting was found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C (2004) The influence of radiation quality on the in vitro rooting and nutrient concentration of peach rootstock GF-677. Biologia Plantarum 48:549–553

    Article  CAS  Google Scholar 

  • Antonopoulou C, Dimassi K, Therios I, Chatzissavvidis C, Tsirakoglou V (2005) Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF-677. Sci. Hortic 106:268–272

    Article  CAS  Google Scholar 

  • Chopra RN, Rashid A (1969) Induction of shoot buds in Anoectangium thomsonii Mitt by a metal chelate Fe-EDDHA. Zeitung Pflanzenphysiolog 61:199–202

    CAS  Google Scholar 

  • Dalton CC, Iqbal K, Turver DA (1983) Iron phosphate precipitation in Murashige and Skoog media. Physiol Plant 57:472– 476

    Article  CAS  Google Scholar 

  • Dimassi-Theriou K: Factors affecting in vitro multiplication and rhizogenesis of the peach rootstock GF 677 and petunia (Petunia hybrida). PhD thesis (In Greek), Aristotle University of Thessaloniki, Greece, pp 18–20 (1989)

  • Dunlap JR, Robacker KM (1988) Nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture. Plant Physiol 88:379–382

    PubMed  CAS  Google Scholar 

  • Foyer C, Rowell J, Walker D (1983) Measurements of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination. Planta 157:239–244

    Article  CAS  Google Scholar 

  • Gaspar T, Kevers C, Hausman JF, Berthon JY, Ripetti V (1992) Practical use of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots. Agronomie 12:757–765

    Article  Google Scholar 

  • Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J 219:1–14

    PubMed  CAS  Google Scholar 

  • Hangarter RP, Stasinopoulos TC (1991) Effect of Fe-catalyzed photooxidation of EDTA on root growth in plant culture media. Plant Physiol 96:843–847

    Article  PubMed  CAS  Google Scholar 

  • Liso R, Innocenti AM, Bitonti MB, Arrigoni O (1988) Ascorbic acid-induced progression of quiescent center cells from G1 to S phase. New Phytol 110:469–471

    Article  CAS  Google Scholar 

  • Maheshwari SC, Seth PN (1965) Induction of flowering in Wolfia microscopica by the iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA). Zeitung Pflanzenphysiolog 55:89–91

    Google Scholar 

  • Molassiotis AN, Dimassi KN, Therios IN, Diamantidis GR (2003) Fe-EDDHA promotes rooting of rootstock GF-677 (Prunus amygdalus × P. persica) explants in vitro. Biol. Plant 47:141–144

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Rashid A, Street HF (1973) The development of haploid embryoids from anther cultures of Atropa belladonna L. Planta 113:263–270

    Article  Google Scholar 

  • Shibli RA, Mohammad MJ, Ajlouni ZI (2002) Growth and micronutrient acquisition of in vitro grown bitter almond and sour orange in response to iron concentration from different iron chelates. J Plant Nutr 25:1599–1606

    Article  CAS  Google Scholar 

  • Singh M, Krikorian AD (1980) Chelated iron in culture media. Ann Bot 46:807–809

    CAS  Google Scholar 

  • Thompson JE, Legge RL, Barber RF (1987) The role of free radicals in senescence and wounding. New Phytol 105:317–344

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture, School of Agriculture, Aristotle University, 541 24, Thessaloniki, Greece

    Chrysovalantou Antonopoulou, Kortessa Dimassi, Ioannis Therios & Ioannis Papadakis

  2. Department of Agricultural Development, Democritus University of Thrace, 68200, Orestiada, Greece

    Christos Chatzissavvidis

Authors
  1. Chrysovalantou Antonopoulou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kortessa Dimassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ioannis Therios
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christos Chatzissavvidis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ioannis Papadakis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kortessa Dimassi.

Additional information

Communicated by E. Lojkowska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Antonopoulou, C., Dimassi, K., Therios, I. et al. The effect of Fe-EDDHA and of ascorbic acid on in vitro rooting of the peach rootstock GF-677 explants. Acta Physiol Plant 29, 559–561 (2007). https://doi.org/10.1007/s11738-007-0067-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-007-0067-9

Keywords

Search

Navigation