Abstract
In this study, seed germination percentages, effects on phases of mitosis and α-amylase enzyme activity of lentil seeds treated with four different concentrations (0.25, 0.5, 1 and 1.5%) of Fusilade (Fluazifop-p-butyl) were determined. Median EC (effective concentration) values were calculated according to seed germination percentages after treatment for 72 h. Germination percentages of primary lentil roots decreased with increasing Fusilade concentrations. Cytological observations showed that the mitotic frequency in root meristematic cells were decreased parallel to the increase in concentrations and all Fusilade concentrations applied decreased the activity of α-amylase enzyme in lentil seeds. The obtained results indicate that the herbicide Fusilade had the ability to cause reduction in seed germination, mitotic frequency and also α-amylase activity of lentil seeds.
Similar content being viewed by others
References
Abdel-Mallek AY, Abdel-Kader MIA, Omar SA (1996) Effect of the herbicide fluazifop-butyl on fungal populations and activity in soil. Water Air Soil Pollut 86:151–157
Aktaç T, Ekinci F, Sıdal U, Sıdal FE (1994) Endosülfanın mercimek (Lens esculanta) kök ucu hücreleri üzerindeki etkileri. Turk J Biol 18:27–37
An YJ (2004) Soil ecotoxicity assessment using cadmium sensitive plants. Environ pollut 127(1):21–26
Bartakova I, Kummerova M, Mandl M, Pospisil M (2001) Phytotoxicity of iron in relation to its solubility conditions and the effect of ionic strength. Plant Soil 235(1):45–51
Beestman GB, Deming JM (1976) Triallate mobility in soils. Weed Sci 24:541–544
Boutin C, Lee HB, Peart ET, Batchelor PS, Maguire RJ (2000) Effects of the sulfonylurea herbicide metsulfuron methyl on growth and reproduction of five wetland and terrestrial plant species. Environ Toxicol Chem 19(10):2532–2541
Chopra S, Singh RP (1978) Effect of gamma rays and 2,4-D on germination, growth and morphogenetic responses in Guizotia abyssinica. Phytomorphology 92(2):82–87
Dalgic O (2005) The determination of some of the toxic effects of Fusilade (Fluazifop-p-butyl) on Lentil (Lens culinaris Medik). PhD thesis, Trakya University, Faculty of Science and Arts, Department of Biology
Dane F, Dalgıc O (2005) The effects of fungicide benomyl (Benlate) on growth and mitosis in onion (Allium cepa L.) root apical meristem. Acta Biologica Hungarica 56(12):119–128
Ekinci F, Aktaç T (1997) Buğday (Triticum aestivum L.) α-Amilazının bazı biyokimyasal özelliklerinin belirlenmesi ve enzim aktivitesi üzerine endosülfanın etkisi. Turk J Biol 21(3):283–298
El-Khodary S, Habib A, Haliem A, 1990) Effect of the herbicide tribunil on root mitosis of Allium cepa. Cytologia 55:209–215
Erlingson M (1988) Fusilade- a strategy for long-term control of couch (Elymus repens). Weeds Weed Control 1:158–165
EXTOXNET (1996) Fluazifop-p-butyl. Pesticide information profiles. Extension toxicology network. http://www.ace.orst.edu/info/extoxnet/
Fayez KA, Gerken I, Kristen U (1994) Ultrastructural responses of root caps to the herbicides chlorsulfuron and metsulfuron methyl. Plant Soil 161:1–8
Fayez KA, Kristen U (1996) The influence of herbides on the root growth and proline content of primary roots and on the ultrastructure of root caps. Environ Exp Bot 36:71–81
Gorlach-Lira K, Stefaniak O, Slizak W, Owedyk I (1997) The response of forest soil microflora to the herbicide formulations Fusilade and Roundup. Microbiol Res 152:319–329
Hess FD (1983) Mode of action of herbicides that affect cell division. In: Miyamoto J, Kearney PC (eds) Pesticide Chemistry. Human welfare and the environment, Pergamon, Oxford 3:79–84
Kim JC, Bendixen LE (1987) Effects of Haloxyfop and CGA-82725 on cell cycle and cell division of oat (Avena sativa) root tips. Weed Sci 35:769–774
Michitte P, Espinoza N, De Prado R (2003) Cross-resistance to ACCase inhibitors of Lolium multiflorum, Lolium perenne and Lolium rigidum found in Chile. Commun Agric Appl Biol Sci 68(4cPtA):397–402
Ouzounidou G, Moustakas M, Eleftheriou EP (1997) Physiological and ultrastructural effects of cadmium on wheat (Triticum aestivum L.) leaves. Arc Env Toxic 32:154–160
Rank J, Nielsen MH (1994) Evaluation of the Allium anaphase-telophase test in relation to genotoxicity screening of industrial wastewater. Mutat Res 312:17–24
Ruiz-Santaella JP, Bakkali Y, Fischer AJ, De Prado R (2003) Is it possible to detect Echinochloa spp. tolerance to ACCase-inhibiting herbicides using a simple quick tolerance test?. Commun Agric Appl Biol Sci 68(4):331–4
Sasaki S, Kozlowski TT, Torrie JH (1968) Effect of pretreatment of pine seeds with herbicides on seed germination and growth of young seedlings. Can J Bot 46(3):255–262
Soliman MI, Ghoneam GT (2004) The mutagenic potentialities of some herbicides using Vicia faba as a biological system. Biotechnology 3(2):140–154
Turk MA, Tawaha AM, El-Shatnawi MKJ (2003) Response of Lentil (Lens culinaris Medik.) to plant density, sowing date, phosphorus fertilization and Ethephon application in the absence of moisture stress. J Agron Crop Sci 189:1–6
Urano K (1982) Onecide, a new herbicide fluazifop-butyl. Jap Pestic Inf 41:28–31
Xiao LY, Hiroshi M (2002) Susceptibility of a broad-leaved weed, Acanthospermum hispidum, to the grass herbicide fluazifop-butyl. Weed Biol Manag 2(2):98–102
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by W. Bielawski.
Rights and permissions
About this article
Cite this article
(Dalgic) Aksoy, O., Dane, F., Ekinci Sanal, F. et al. The effects of Fusilade (Fluazifop-p-butyl) on germination, mitotic frequency and α-amylase activity of lentil (Lens culinaris Medik.) seeds. Acta Physiol Plant 29, 115–120 (2007). https://doi.org/10.1007/s11738-006-0014-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11738-006-0014-1