Abstract
The major constrains for practical exploitation of the somatic hybrids between eggplant and its wild relatives have been their sterility and tetraploidy which prevented their incorporation into breeding programs. Here we demonstrate that anther culture was successfully utilized to bring back the ploidy level to the diploid status in tetraploid interspecific hybrids between eggplant and the allied species S. integrifolium and S. aethiopicum gr. gilo. Both the relative species are resistant to Fusarium oxysporum f. sp. melongenae and to some strains of bacterial wilt (Ralstonia solanacearum) which are very destructive diseases of eggplant. Dihaploid androgenetic plants were obtained from the somatic hybrids, from the “double somatic hybrid” obtained by sexual cross of the two somatic hybrids [(eggplant + S. aethiopicum) × (eggplant + S. integrifolium)], and from tetraploid backcrossed plants between the somatic hybrid with S. aethiopicum and eggplant. Phenotypical, molecular, biological and biochemical characterization, and also artificial inoculation with Fusarium oxysporum are consistent with a recombination between the genomes of the species involved in the hybridizations. Dihaploids resistant to Fusarium were successfully backcrossed with eggplant. Besides their utility as potential valuable breeding materials, the introgressed lines obtained may be utilized in genetic and molecular studies about the resistance to Fusarium from S. integrifolium and S. aethiopicum gr. gilo.
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Bletsos F.A., Roupakias D.G., Tsaktsina M.L., Scaltsojannes A.B., Thanassoulopoulos C.C. 1998. Interspecific hybrids between three eggplant (S. melongena L.) cultivars and two wild species (S. torvum Sn & S. sisymbriifolium). Plant Breed. 117: 159–164.
Burnham C.R. 1967. Discussion in cytogenetics. Burgess Publishing Company Minneapolis, Minnesota. pp 203–250.
Cappelli C., Stravato V.M., Rotino G.L., Buonaurio R. 1995. Sources of resistance among Solanum spp. to an Italian isolate of Fusarium oxysporum f. sp. melongenae, In: Eucarpia, IX Meeting Genet. Breed. Capsicum Eggplant, ed. by A. Andrasfalvi, A. Moor, J. Zatyko, Sincop, Budapest: 221–224.
Collonnier C., Mulya, K., Fock, I., Mariska, I., Servaes, A., Vedel, F., Siljak-Yakovlev., Souvannavong, V., Ducreux, G., Sihachakr, D. 2001a. Source of resistance against Ralstonia solanaceraum in fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L. Plant Sci. 301–313.
Collonnier C., Fock I., Kashyap V., Rotino G.L., Daunay M.C., Lian Y., Mariska I.K., Rajam M.V., Servaes A., Ducreux G., Sihachakr D. 2001b. Applications of biotechnology in eggplant. Plant Cell Tiss. Org. Cult. 65: 91–107.
Daunay M.C, Lester R.N., Laterrot H. 1991. The use of wild species for the genetic improvement of Brinjal eggplant (Solanum melongena) and tomato (Lycopersicum esculentum). In: Solanaceae III: Taxonomy, Chemistry, Evolution, Vol 27, ed. by J.C. Hawkes, R.N. Lester, M. Nee, N. Estrada, Royal Botanic Gardens Kew and Linnean Soc., London: 389–413.
Daunay M.C., Chaput M.H., Sihachakr D., Allot M., Vedel F., Ducreux G. 1993. Production and characterization of fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L. Theor. Appl. Genet. 85: 841–850.
Dumas de Vaulx R., Chambonnet D. 1982. Culture in vitro d’anth res d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements +35°C associés à de faibles teneurs en substances de croissance. Agronomie 2: 983–988.
Fock I., Collonnier C., Purwito A., Luisetti J., Souvannavong V., Vedel F., Servaes A., Ambroise A., Kodja H., Ducreux G., Sihachakr D. 2001. Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiol. Biochem. 39: 899–908.
Gleddie S., Keller W.A, Setterfield G. 1986. Production and characterisation of somatic hybrids between Solanum melongena L. and S. sisymbrifolium. Theor. Appl. Genet. 71: 613–621.
Isshiki, S., Okubo, H., Fujieda, K. 2000. Segregation of isozymes in selfed progenies of a synthetic amphidiploid between Solanum integrifolium and S. melongena. Euphytica, 112: 9–14.
Lefrancois C.Y., Chupeau Y., Bourgin J.P. 1993. Sexual and somatic hybridisation in the genus Lycopersicon. Theor. Appl. Genet. 86: 533–546.
Miyoshi K. 1996. Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep. 15: 391–395.
Raina S.K., Iyer R.D. 1973. Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L. Z. Pflanzenzucht. 70: 275.
Rizza F., Mennella G., Collonnier C., Sihachakr D., Kashyap V., Rajam M.V., Presterà M., Rotino G.L. 2002. Androgenic dihaploids from somatic hybrids between Solanum melongena and Solanum aethiopicum group gilo as source of resistance to Fusarium oxysporum f. sp. melongenae. Plant Cell Rep. 20: 1022–1032.
Rotino G.L. 1996. Haploidy in Eggplant. In: In vitro Haploid Production in Higher Plants, Vol. 3, ed. by S.M. Jain, S.K. Sopory, R.E. Veilleux, Kluwer Acad. Publishers, The Netherlands: 115–141.
Rotino G.L., Perri E., D’Alessandro A., Mennella G. 1998. Characterization of fertile somatic hybrids between eggplant (S. melongena L.) and S. integrifolium Poir. Xth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant, 7–11 September 1998, Avignon-France; 213–217.
Rotino G.L., Mennella G., Fusari F., Vitelli G., Tacconi M.G., D’Alessandro A., Acciarri N. 2001. Towards introgression of resistance to Fusarium oxysporum f. sp. melongenae from Solanum integrifolium into eggplant. XIth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant, April 9–13, 2001, Antalya-Turkey; pp 303–307.
Rotino G.L., Rizza F., Mennella G., Tacconi M.G., Alberti P., D’Alessandro A., Acciarri N., Toppino L., 2004. Production and utilization of sexual “doubled hybrid” between the somatic hybrids S. melongena (+) S. integrifolium and S. melongena (+) S. aethiopicum gr. gilo. EUCARPIA, XIIth Meeting on Genetics and Breeding of Capsicum & Eggplant. Noordwijkerhout-The Netherlands, 17–19 May 2004; pp 203–209.
Sihachakr D., Daunay M.C., Serraf I., Chaput M.H., Mussio I., Haicour R., Rossignol L., Ducreux G. 1994. Somatic hybridization of eggplant (Solanum melongena L.) with its close and wild relatives. In: Biotechnology in Agriculture and Forestry, Somatic hybridization in Crop Improvement, Vol 1, ed. by Y.P.S. Bajaj, Springer-Verlag, Berlin, Heidelberg: 255–278.
Toppino L. 2002. Analisi biomorfologica e molecolare di piante androgenetiche ottenute da “doppi ibridi” [(S. melongena+ S. aethiopicum gr. gilo) × (S. melongena + S. integrifolium)] e sviluppo di marcatori molecolari per l’introgressione della resistenza a Fusarium oxysporum in melanzana. Bachelor thesis, University of Milano.
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Rotino, G.L., Sihachakr, D., Rizza, F. et al. Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives. Acta Physiol Plant 27, 723–733 (2005). https://doi.org/10.1007/s11738-005-0077-4
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DOI: https://doi.org/10.1007/s11738-005-0077-4