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Effect of Mg2+ concentration on biocompatibility of pure magnesium

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Abstract

The aim of the present study is to find the correlation between the Mg2+ concentration degraded from pure magnesium material and the biocompatibility of the material. Hemolysis ratio (HR) of the extracts of pure magnesium with different Mg2+ concentration were measured according to ISO 10993.4 standard. The cytotoxicity tests were carried out by both indirect contact with fibroblast L929 and preosteoblasts MC3T3-E1, and MTT tests were used. Cytotoxicity of the pure magnesium with and without surface modification was further evaluated by direct contact method. Samples were cultured with Osteoblast MC3T3-E1 and the effects of the material on viability and activity of cells were discussed. The results showed that the hemolysis rate and cytotoxicity of the modified Mg could meet the requirement for biomaterials. In our test, the hemolysis rate of the extracts was qualified when the concentration of Mg2+ ⩽ 42 mg/L; the extracts with 202 mg/L Mg2+ met the cytotoxicity requirement, and the extracts with 156 mg/L Mg2+ promoted cell proliferation. Therefore, the biocompatibility of magnesium-based materials can be improved by suitable surface modification.

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Authors and Affiliations

  1. National Engineering Center for Magnesium Alloy, Chongqing University, Chongqing, 400044, China

    Jia-Cheng Gao & Li-Ying Qiao

  2. College of Material Science and Engineering, Chongqing University, Chongqing, 400045, China

    Jia-Cheng Gao, Li-Ying Qiao & Ren-Long Xin

Authors
  1. Jia-Cheng Gao
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  2. Li-Ying Qiao
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  3. Ren-Long Xin
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Correspondence to Jia-Cheng Gao.

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Gao, JC., Qiao, LY. & Xin, RL. Effect of Mg2+ concentration on biocompatibility of pure magnesium. Front. Mater. Sci. China 4, 126–131 (2010). https://doi.org/10.1007/s11706-010-0030-3

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  • DOI: https://doi.org/10.1007/s11706-010-0030-3

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