Abstract
In this work, essential oil was extracted from nettle leaves and encapsulated in chitosan nanoparticles by a two-stage of emulsion-ionic gelation method. An acceptable retention rate (59.5 to 68.2%) of nettle essential oil (NEO) loaded in chitosan nanoparticles was achieved. Also, Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) also confırmed successful introducing and loading NEO in chitosan. The nanoparticles displayed an average size of 208.3–369.4 nm. NEO-loaded chitosan nanoparticles had greater antioxidant activity than the free form of NEO. Furthermore, the antibacterial activity of NEO-loaded chitosan nanoparticles was high against S. aureus and E. coli (inhibition zone diameter of 4.11–3.95 cm). Based on these results, the encapsulation of EOs in chitosan nanoparticles are promising candidate to be used in food and pharmaceutical products for novel applications.
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A.E. Asbahani, K. Miladi, W. Badri, M. Sala, E.H.A. Addi, H. Casabianca, A.E. Mousadik, D. Hartmann, A. Jilale, F.N.R. Renaud, A. Elaissari, Essential oils: from extraction to encapsulation. Int. J. Pharm. 483, 220–243 (2015)
A. Esfahani, M. Ehsani, M. Mizani, A.M. Nafchi, The synergistic effects of cinnamon essential oil and nano TiO2 on antimicrobial and functional properties of sago starch films. Int. J. Biol. Macromol. 157, 743–751 (2020)
S.M.T. Gharibzahedi, S. Mohammadnabi, Effect of novel bioactive edible coatings based on jujube gum and nettle oil-loaded nanoemulsions on the shelf-life of Beluga sturgeon fillets. Int. J. Biol. Macromol. 95, 769–777 (2017)
F. Donsì, M. Annunziata, M. Sessa, G. Ferrari, Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT Food Sci. Technol. 44, 1908–1914 (2011)
N. Vahedikia, F. Garavand, B. Tajeddin, I. Cacciotti, S.M. Jafari, T. Omidi, Z. Zahedi, Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: physical, mechanical, structural and antimicrobial attributes. Colloids Surf. B. 177, 25–32 (2019)
E. Assadpour, S.M. Jafari, A systematic review on nanoencapsulation of food bioactive ingredients and nutraceuticals by various nanocarriers. Crit. Rev. Food Sci. Nutr. 59(19), 3129–3151 (2019)
M. Hadidi, S. Pouamin, F. Adinepour, S. Haghani, S.M. Jafari, Chitosan nanoparticles loaded with clove essential oil: characterization, antioxidant and antibacterial activities. Carbohydr. Polym. 236, 116075 (2020)
D.S. Do Amaral, A. Cardelle-Cobas, B.M. Do Nascimento, M.J. Monteiro, M.S. Madruga, M.M. Pintado, Development of a low fat fresh pork sausage based on chitosan with health claims: Impact on the quality, functionality and shelf-life. Food Funct. 6(8), 2768–2778 (2015)
P. Estakhr, J. Tavakoli, F. Beigmohammadi, S. Alaei, A. Mousavi Khaneghah, Incorporation of the nanoencapsulated polyphenolic extract of Ferula persica into soybean oil: assessment of oil oxidative stability. Food Sci. Nutr. 8(6), 2817–2826 (2020)
I. Younes, M. Rinaudo, Chitin and chitosan preparation from marine sources Structure, properties and applications. Mar. Drugs. 13(3), 1133–1174 (2015)
M. Arteche Pujana, L. Pérez-Álvarez, L.C. Cesteros Iturbe, K. Issa, Biodegradable chitosan nanogels crosslinked with genipin. Carbohydr. Polym. 94(2), 836–842 (2013)
D. Ramimoghadam, S. Bagheri, S.B.A. Hamid, Stable monodisperse nanomagnetic colloidal suspensions: an overview. Colloids Surf. B. 133, 388–411 (2014)
A. Esmaeili, A. Asgari, In vitro release and biological activities of Carum copticum essential oil (CEO) loaded chitosan nanoparticles. Int. J. Biol. Macromol. 81, 283–290 (2015)
S.F. Hosseini, M. Zandi, M. Rezaei, F. Farahmandghavi, Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: preparation, characterization and in vitro release study. Carbohydr. Polym. 95, 50–56 (2013)
B. Jamil, R. Abbasi, S. Abbasi, M. Imran, S.U. Khan, A. Ihsan, S. Javed, H. Bokhari, Encapsulation of cardamom essential oil in chitosan nano-composites: in-vitro efficacy on antibiotic-resistant bacterial pathogens and cytotoxicity studies. Front. Microbiol. 7, 1580 (2016)
R.P. Adams, Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th edn. (Allured Publishing Corp, Carol Stream, 2007)
A. Gasparetto, A. Bella Cruz, T.M. Wagner, T.J. Bonomini, R. Correa, A. Malheiros, Seasonal variation in the chemical composition, antimicrobial and mutagenic potential of essential oils from Piper cernuum. Ind. Crops Prod. 95, 256–263 (2017)
L. Keawchaoon, R. Yoksan, Preparation characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles. Colloids Surf. B 84, 163–171 (2011)
N. Hasheminejad, F. Khodaiyan, M. Safari, Improving the antifungal activity of clove essential oil encapsulated by chitosan nanoparticles. Food Chem. 275, 113–122 (2019)
M. Hadidi, A. Ibarz, S. Pouramin, Optimization of extraction and deamidation of edible protein from evening primrose (Oenothera biennis L.) oil processing by-products and its effect on structural and techno-functional properties. Food Chem. 334, 127613 (2021)
M. Hadidi, P. Izadi Amoli, A. Zarei Jelyani, Z. Hasiri, A. Rouhafza, A. Ibarz, F. Baradaran Khaksar, S. Tajbakhsh, Polysaccharides from pineapple core as a canning by-product: extraction optimization, chemical structure, antioxidant and functional properties. Int. J. Biol. Macromol. 163, 2357–2364 (2020)
M. Sotelo-Boyása, Z. Correa-Pachecoa, S. Bautista-Banosa, Y. Gómez, Release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria. Int. J. Biol. Macromol. 103, 409–414 (2017)
S. Gul, B. Demirci, K.H.C. Baser, H.A. Akpulat, P. Aksu, Chemical composition and in vitro cytotoxic, genotoxic effects of essential oil from Urtica dioica L. Bull. Environ. Contam. Toxicol. 88(5), 666–671 (2012)
S. Das, V.K. Singh, A.K. Dwivedy, A.K. Chaudhari, N. Upadhyay, P. Singh, S. Sharma, N.K. Dubey, Encapsulation in chitosan-based nanomatrix as an efficient green technology to boost the antimicrobial, antioxidant and in situ efficacy of Coriandrum sativum essential oil. Int. J. Biol. Macromol. 133, 294–305 (2019)
K.I. Matshetshe, S. Parani, S.M. Manki, O.S. Oluwafemi, Preparation, characterization and in vitro release study of β-cyclodextrin/chitosan nanoparticles loaded Cinnamomum zeylanicum essential oil. Int. J. Biol. Macromol. 118(PartA), 676–682 (2018)
S. Hasani, S.M. Ojagh, M. Ghorbani, Nanoencapsulation of lemon essential oil in Chitosan-Hicap system. Part 1: study on its physical and structural characteristics. Int. J. Biol. Macromol. 115, 143–151 (2018)
E. Dickinson, Hydrocolloids as emulsifiers and emulsion stabilizers. Food Hydrocoll. 23, 1473–1482 (2009)
G.C. Feyzioglu, F. Tornuk, Development of chitosan nanoparticles loaded with Summer savory (Satureja hortensis L.) essential oil for antimicrobial and antioxidant delivery applications. LWT-Food Sci. Technol. 70, 104–110 (2016)
P. Lertsutthiwong, P. Rojsitthisak, U. Nimmannit, Preparation of turmeric oil-loaded chitosan-alginate biopolymeric nanocapsules. Mater. Sci. Eng. C. 29(3), 856–860 (2009)
M. Hasani, A.H. Elhami Rad, M.M. Hosseini, M. Shahidi Noghabi, Physicochemical characteristic of microencapsulated fish oil by freeze-drying using different combinations of wall materials. Biosci. Biotechnol. Biochem. 12, 45–51 (2015)
R. Yoksan, J. Jirawutthiwongchai, K. Arpo, Encapsulation of ascorbyl palmitate in chitosan nanoparticles by oil-in-water emulsion and ionic gelation processes. Colloids Surf. B 76, 292–297 (2010)
A. Shetta, J. Kegere, W. Mamdouh, Comparative study of encapsulated peppermint and green tea essential oils in chitosan nanoparticles: encapsulation, thermal stability, in-vitro release, antioxidant and antibacterial activities. Int. J. Biol. Macromol. 126, 731–742 (2018)
K. Pan, Y. Luo, Y. Gan, S.J. Baek, Q. Zhong, pH-driven encapsulation of curcumin in self-assembled casein nanoparticles for enhanced dispersibility and bioactivity. Soft Matter 10(35), 6820–6830 (2014)
S. Woranuch, R. Yoksan, Eugenol-loaded chitosan nanoparticles: I. Thermal stability improvement of eugenol through encapsulation. Carbohydr. Polym. 96, 578–585 (2013)
M.S. Daneshzadeh, H. Abbaspour, L. Amjad, An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri F Delaroche. J. Food Meas. Charact. 14, 708–715 (2020)
S. Roshanpour, J. Tavakoli, F. Beigmohammadi et al., Correction to: Improving antioxidant effect of phenolic extract of Mentha piperita using nanoencapsulation process. J. Food Meas. Charact. (2020). https://doi.org/10.1007/s11694-020-00631-w
A.M. Nafchi, A. Olfat, M. Bagheri, L. Nouri, A. Karim, F. Ariffin, Preparation and characterization of a novel edible film based on Alyssum homolocarpum seed gum. J. Food Sci. Technol. 54(6), 1703–1710 (2017)
M.Z. Elsabee, E.S. Abdou, Chitosan based edible films and coatings: a review. Mater. Sci. Eng. C 33, 1819–1841 (2013)
J. Seil, T. Webster, Antimicrobial applications of nanotechnology: methods and literature. Int. J. Nanomed. 7, 2767–2781 (2012)
H. Zhang, J. Jung, Y. Zhao, Preparation, characterization and evaluation of antibacterial activity of catechins and catechins-Zn complex loaded chitosan nanoparticles of different particle sizes. Carbohydr. Polym. 137, 82–91 (2016)
D. Raafat, K.V. Bargen, A. Haas, H.G. Sahl, Insights into the mode of action of chitosan as an antibacterial compound. Appl. Environ. Microbiol. 74(12), 3764–3773 (2008)
S. Burt, Essential oils: their antibacterial properties and potential applications in foods: a review. Int. J. Food Microbiol. 94, 223–253 (2004)
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Bagheri, R., Ariaii, P. & Motamedzadegan, A. Characterization, antioxidant and antibacterial activities of chitosan nanoparticles loaded with nettle essential oil. Food Measure 15, 1395–1402 (2021). https://doi.org/10.1007/s11694-020-00738-0
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DOI: https://doi.org/10.1007/s11694-020-00738-0