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Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats

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Abstract

Glucagon-like peptide-2 (GLP-2) has potent anti-inflammatory effects and protects against experimental ischemia/reperfusion (I/R) injury in pulmonary, intestinal, and myocardial tissue. However, its protective abilities against I/R injury in the liver are unknown. We investigated the potential role of GLP-2 pretreatment on hepatic I/R injury in rats. A total of 24 rats were randomly divided into three groups (n = 8). The first group was the control group; the second group was the vehicle-treated hepatic ischemia/reperfusion (HIR, vehicle saline-treated) group; and the third group was the GLP-2 pretreated I/R (GLP2-IR) group. Each rat in the third group was intraperitoneally administered 5 µg GLP-2 for 5 d before the procedure. A portal triad was created to induce ischemia with a vascular atraumatic clamp. After 40 min, the clamp was released to initiate hepatic reperfusion for 6 h. Blood samples and tissue specimens from the liver were obtained. Alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels significantly increased in the salinetreated HIR group (P < 0.001), whereas GLP-2 pretreatment significantly decreased their levels (P < 0.01). Our data suggested that GLP-2 pretreatment may have a protective effect on liver I/R injury. However, dose-response studies are necessary to determine the most effective dose.

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Authors and Affiliations

  1. Medical Faculty, Department of Pediatrics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Naci Topaloğlu, Şule Yıldırım & Mustafa Tekin

  2. Department of Pediatric Surgery, Düzce Atatürk State Hospital, Düzce, Turkey

    Adem Küçük

  3. Medical Faculty, Department of Pathology, Düzce University, Düzce, Turkey

    Havva Erdem

  4. Medical Faculty, Department of Physiology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Mustafa Deniz

Authors
  1. Naci Topaloğlu
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  2. Adem Küçük
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  3. Şule Yıldırım
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  4. Mustafa Tekin
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  5. Havva Erdem
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  6. Mustafa Deniz
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Correspondence to Şule Yıldırım.

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Topaloğlu, N., Küçük, A., Yıldırım, Ş. et al. Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats. Front. Med. 9, 368–373 (2015). https://doi.org/10.1007/s11684-015-0403-1

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  • DOI: https://doi.org/10.1007/s11684-015-0403-1

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