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Protective effect of tanshinone II A on signal transduction system protein kinase B in rats with myocardial hypertrophy

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Abstract

The effects of tanshinone II A on cell signal transduction system protein kinase B in rats with myocardial hypertrophy induced by the abdominal aorta partial coarctation were investigated. Rat models of myocardial hypertrophy were established by using abdominal aorta partial coarctation method. Forty-eight rats were randomly divided into sham group (S group), model group (M group), valsartan treatment group (X group), low-dose tanshinone treatment group (LD group), medium-dose tanshinone treatment group (MD group), and high-dose tanshinone treatment group (HD group) (n = 8 in each group). Left ventricular mass index (LVMI), left ventricular posterior wall (LVPW), and septal thickness (IVS) were detected by high frequency ultrasonography. Myocardial fiber diameter (MFD) was examined by Hematoxylin-Eosin (HE) staining, and the contents of phosphorylated protein kinase B (p-Akt) and p-Gsk3β in myocardium were assayed by Western blot. The results showed that compared with S group, the values of LVMI, LVPW, IVS and MFD were increased in other groups (P<0.05), and the contents of p-Akt, and p-Gsk3β were also increased in other groups. As compared with MD group, the values of LVMI, LVPW, IVS and MFD were decreased in all treatment groups (P<0.05), and the contents of p-Akt, and p-Gsk3β were also decreased in all treatment groups. However, there were no significant differences among LD, MD, and HD groups (P>0.05), and there were no significant differences between X group and tanshinone treatment groups (P>0.05). It was suggested that tanshinone II A could prevent myocardial hypertrophy by its action on the Akt signaling pathway.

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Authors and Affiliations

  1. Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Enyuan Tu, Yongjun Pan, Kang Zheng, Zhaohua Wang, Qiansheng Liang & Guangtian Yang

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  1. Enyuan Tu
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  2. Yongjun Pan
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  3. Kang Zheng
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  4. Zhaohua Wang
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  6. Guangtian Yang
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Correspondence to Guangtian Yang.

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Tu, E., Pan, Y., Zheng, K. et al. Protective effect of tanshinone II A on signal transduction system protein kinase B in rats with myocardial hypertrophy. Front. Med. China 3, 431–436 (2009). https://doi.org/10.1007/s11684-009-0088-4

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  • DOI: https://doi.org/10.1007/s11684-009-0088-4

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