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Comparative microsporogenesis and flower development in Eucalyptus urophylla × E. grandis

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Abstract

Microsporogenesis and flower development in Eucalyptus urophylla × E. grandis were examined using chromosome tableting to provide a method to predict the meiotic stages in this species. Although microsporogenesis was normal, cytokinesis during meiosis of pollen mother cells occurred simultaneously, with strong asynchronism observed in the two different lengths of stamens in a flower bud. In a single flower, the developmental period of microsporogenesis in anthers on the longer stamens was always ahead of those on the shorter stamens. Flower development was also asynchronous at different locations on a branch. Flower buds on the upper side of the branch were larger in diameter than those on the lower side. In addition, a correlation was observed between microsporogenesis development and flower bud diameter growth. The pachytene stage was first observed when the diameter of the flower buds increased to 3.0 mm, and the majority of the meiotic stages were observed when bud diameters ranged from 3.5 to 5.0 mm. This study showed that the developmental stages of microsporogenesis in Eucalyptus urophylla × E. grandis could be distinguished readily, which may be applicable to future breeding studies.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, People′s Republic of China

    Jun Yang, Pengqiang Yao, Zhen Huang & Xiangyang Kang

  2. Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, 100083, People′s Republic of China

    Jun Yang, Pengqiang Yao, Zhen Huang & Xiangyang Kang

  3. Guangxi Dongmen Forest Farm, Guangxi Zhuang Autonomous Region, 532108, People′s Republic of China

    Jun Lan

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  1. Jun Yang
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  2. Jun Lan
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  3. Pengqiang Yao
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  4. Zhen Huang
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  5. Xiangyang Kang
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Correspondence to Xiangyang Kang.

Additional information

Project funding: This research was financially supported by the Special Fund for Forest Scientific Research in the Public Welfare (201404113), the 111 Project (B13007) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT13047).

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Corresponding editor: Hu Yanbo

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Yang, J., Lan, J., Yao, P. et al. Comparative microsporogenesis and flower development in Eucalyptus urophylla × E. grandis . J. For. Res. 27, 257–263 (2016). https://doi.org/10.1007/s11676-015-0150-9

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  • DOI: https://doi.org/10.1007/s11676-015-0150-9

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