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Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury

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Abstract

Objective

To investigate the synergistic effect of Naoxintong Capsule (NXTC, 脑心通胶囊) and Guhong Injection (GHI, 谷红注射液) on cerebral ischemia-reperfusion (I/R) injury.

Methods

Forty-eight Sprague-Dawley rats were divided into 6 groups: control group, oxygen and glucose deprivation (OGD) group, nimodipine group (9.375 mg/kg), NXTC group (0.5 g/kg), GHI group (5 mL/kg) and NXTC+GHI group (0.5 g/kg NXTC+5 mL/kg GHI), after the onset of reperfusion and once per day for the following 7 days. Blood was collected 1 h after final administration, and the sera were collected. Cultured primary rat brain microvascular endothelial cells (rBMECs) were subjected to OGD to establish a cell injury model. Untreated rBMECs were used as blank control. The cell counting kit-8 assay was used to assess cell viability using the sera. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assessed using an enzyme-linked immunosorbent assay. Apoptosis was evaluated after Hoechst33342 staining using fluorescence microscopy and flow cytometry. JC-1 staining was performed to assess changes in mitochondrial membrane potential.

Results

Statistical analysis indicated that more than 95% of the cells were rBMECs. Compared with the OGD group, the cellular morphology of the all drug delivery groups improved. In particular, the combined drug group had the most significant effect. Compared with the OGD group, all drug intervention groups induced a decrease in the apoptotic rate of rBMECs, increased the SOD levels, and decreased the MDA levels (all P<0.01). Compared with the mono-therapy groups, the NXTC+GHI group exhibited a significant improvement in the number of apoptotic rBMECs (P<0.01). All drug intervention groups showed different degrees of increase in membrane potential, and the NXTC+GHI group was higher than the NXTC or GHI group (P<0.01).

Conclusion

The combinationa application of NXTC and GHI on cerebral I/R injury clearly resulted in protective benefits.

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Author information

Authors and Affiliations

  1. College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Hai-yan Wang, Hui-fen Zhou, Li Yu, Chang Li & Hai-tong Wan

  2. College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Yu He

  3. College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou, 310053, China

    Jie-hong Yang

Authors
  1. Hai-yan Wang
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  2. Hui-fen Zhou
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  3. Yu He
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  4. Li Yu
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  5. Chang Li
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Contributions

Wan HT and Yang JH designed the experiments. Wang HY, Zhou HF and He Y perform the experiments. Yu L and Li C analyzed the data. Wang HY wrote the manuscript. Wang HT and Yang JH supervise the whole experimental process and the preparation of the manuscript. All authors read and approved the final manuscript for publication.

Corresponding author

Correspondence to Hai-tong Wan.

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The authors have no financial or commercial conflicts of interest to declare.

Additional information

Supported by the National Natural Science Foundation of China (No. 81630105, 81973560); Zhejiang Provincial Natural Science Foundation of China (Nos. LZ17H270001 and LZ18H270001) and Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents

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Wang, Hy., Zhou, Hf., He, Y. et al. Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury. Chin. J. Integr. Med. 27, 744–751 (2021). https://doi.org/10.1007/s11655-020-3215-3

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  • DOI: https://doi.org/10.1007/s11655-020-3215-3

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