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Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways

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Abstract

Objective

To investigate the effect of salvianolic acid B (Sal B) on oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs) apoptosis and the possible mechanism.

Methods

HUVECs were divided into 6 groups, including control group, ox-LDL group, vitamin C group (positive control), and 5, 10 and 20 μg/mL Sal B groups. Cell viability of HUVECs was determined by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The anti-apoptotic effect of Sal B was tested by Hoechst 33258 staining and Annexin V/propidium iodide flflow cytometry analysis. Apoptosis-related genes (p53, Bcl-2 and Bax) expression and caspase-3 activity were also determined. Oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by the corresponding kits.

Results

In HUVECs, ox-LDL signifificantly reduced cell viability and induced apoptosis (P<0.05 or P<0.01), however, Sal B diminished the effects of ox-LDL in a dose-dependent manner (P<0.05). Moreover, 10 and 20 μg/mL Sal B reduced the expression levels of p53, increased the Bcl-2/Bax ratio and inhibited the caspase-3 activity in ox-LDL-treated HUVECs (P<0.05). In addition, 5, 10 and 20 μg/mL Sal B signifificantly enhanced the activity of SOD, while decreased the level of MDA in the HUVECs which treated with ox-LDL (P<0.05).

Conclusion

Sal B exhibited anti-apoptotic effects in ox-LDL-induced endothelial cell injury by suppressing oxidative stress, p53, and caspase-3.

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Acknowledgments

We thank Jessica Waite (Biology Department, Penn State University) and Erin D. Scully (Huck Institutes of the Life Sciences, Penn State University) for their assistance.

Author information

Authors and Affiliations

  1. Key Laboratory of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China

    Hong-mei Chen, Hao Luo, Wen-bi Zeng, Bin Liu, Xue-gang Sun & Ying-chun Zhou

  2. Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China

    Hong-mei Chen & Ying-chun Zhou

  3. The Fifth Department of Internal Medicine, Dongguan Hospital of Traditional Chinese Medicine, Dongguan, Guangdong Province, 523000, China

    Jia-cheng Huang & Yan-jin Zeng

  4. Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China

    Min Liu

  5. Department of Traditional Chinese Medicine, Shantou Central Hospital, Shantou, Guangdong Province, 515031, China

    Qiang Zheng

  6. Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China

    Ji-qiang Li

Authors
  1. Hong-mei Chen
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  2. Hao Luo
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  3. Wen-bi Zeng
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  4. Bin Liu
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  5. Jia-cheng Huang
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  6. Min Liu
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  8. Qiang Zheng
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  9. Ji-qiang Li
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  10. Xue-gang Sun
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Corresponding author

Correspondence to Ying-chun Zhou.

Additional information

Supported by the National Natural Science Foundation of China (Nos. 30973850, 81173459, and 81202841) and Planned Science and Technology Project of Guangdong Province (No. 2010B060500009), China

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Chen, Hm., Luo, H., Zeng, Wb. et al. Salvianolic acid B attenuates oxidized low-density lipoprotein-induced endothelial cell apoptosis through inhibition of oxidative stress, p53, and caspase-3 pathways. Chin. J. Integr. Med. (2017). https://doi.org/10.1007/s11655-016-2645-4

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  • DOI: https://doi.org/10.1007/s11655-016-2645-4

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