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Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint

  • Acupuncture Research
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Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the effect of direct moxibustion at Ganshu (BL18) on the serum concentrations of tumor specific growth factor (TSGF) and tumor necrosis factor α (TNF-α) in a rat model with precancerous lesion of primary hepatocellular carcinoma (HCC), so as to explore the mechanism of moxibustion underlying improvement of HCC.

Methods

Sixty male Wistar rats were randomly divided into control group (n=10), model group (n=20), prevention group 1 (n=15) and prevention group 2 (n=15). The normal rats were injected with physiological saline as blank control. At the same time, the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibustion with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1 (1 treatment course, 20 days) and prevention group 2 (2 treatment courses, 40 days), 5 doses for each acupoint, 0.5 mg/dose, once every other day. At each time point (before model establishment, the end of 1st course prevention, the end of 2nd course prevention and the end of model establishment), serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay.

Results

Compared with the control group, there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment (P<0.05). At the end of the 1st course of direct moxibustion, the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group (P<0.05). At the end of the 2nd course of direct moxibustion, serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference (P<0.05), and the levels of TSGF and TNF-α in the prevention group 2 were significantly reduced in comparison with the model group (P<0.05).

Conclusion

It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis, and the therapeutic effect of two courses were better than one course.

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Authors and Affiliations

  1. School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 100029, China

    Pei Wang  (王 培), Jiang Zhu  (朱 江), Ming-he Sui  (睢明河), Wen-rui Jia  (贾文睿), Yang Liu  (柳 杨) & Zhong-wei Hou  (侯中伟)

  2. The Key Unit of Evaluation of Characteristic Acupuncture Therapy, State Administration of Traditional Chinese Medicine, Beijing, 100029, China

    Jiang Zhu  (朱 江)

  3. The Research Institute of Acupuncture and Moxibustion in Houma City, Houma, Shanxi Province, 043001, China

    Xi-liang Xie  (谢锡亮)

  4. School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China

    Qiu-ju Zhang  (张秋菊)

  5. Chengde Medical College, Chengde, Hebei Province, 067000, China

    Si-yuan Xin  (辛思源)

Authors
  1. Pei Wang  (王 培)
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  2. Jiang Zhu  (朱 江)
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  3. Xi-liang Xie  (谢锡亮)
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  4. Ming-he Sui  (睢明河)
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  5. Qiu-ju Zhang  (张秋菊)
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  6. Wen-rui Jia  (贾文睿)
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  7. Si-yuan Xin  (辛思源)
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  8. Yang Liu  (柳 杨)
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  9. Zhong-wei Hou  (侯中伟)
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Corresponding author

Correspondence to Zhong-wei Hou  (侯中伟).

Additional information

Supported by the National Natural Science Foundation of China (No. 81202760) and the Doctoral Program of Higher Educition of Ministry Education of China (No. 20100013120014)

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Wang, P., Zhu, J., Xie, Xl. et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint. Chin. J. Integr. Med. 22, 532–536 (2016). https://doi.org/10.1007/s11655-015-2157-7

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  • DOI: https://doi.org/10.1007/s11655-015-2157-7

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