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Effect of Compound Zhebei Granule (复方浙贝颗粒) combined with chemotherapy on surface markers of leukemia stem cell in patients with acute myeloid leukemia

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Abstract

Objective

To observe the effects of Compound Zhebei Granule (复方浙贝颗粒, CZBG) combined with chemotherapy on surface markers of leukemia stem cell (LSC) in the bone marrow of patients with acute myeloid leukemia (AML).

Methods

Seventy-eight patients with AML received bone marrow aspiration and the percentages of CD34+ CD123+ and CD33+ CD123+ cells were tested using flow cytometry method. A total of 24 refractory or relapsed AML patients were enrolled and treated with one cycle of standard chemotherapy combined with CZBG. Bone marrow samples were obtained before and after treatment, and the percentages of CD34+ CD123+ and CD33+ CD123+ cells were examined by flflow cytometry.

Results

Compared with refractory or relapsed AML patients, patients achieved remission had a significant lower percentage of CD34+ CD123+ cells(P<0.01) and CD33+ CD123+ cells (P<0.01), indicating that controlling the LSC percentage may be important for patients with AML to achieve sustainable remission. Compared with those before treatment, the expression levels of CD34+ CD123+ were significantly decreased after CZBG combined with chemotherapy treatment (P<0.01). The percentages of CD34+ CD123+ cells and CD33+ CD123+ in patients achieving complete remission after CZBG combined with chemotherapy treatment were both significantly lower than those in patients with nonremission (P<0.01).

Conclusion

CZBG combining chemotherapy could reduce the percentages of CD34+ CD123+ and CD33+ CD123+ LSC, which might improve the clinical efficacy of refractory or relapsed AML.

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Authors and Affiliations

  1. Department of Oncology and Hematology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100700, China

    Jing Wang  (王 婧), Zong-lang Lai  (赖宗浪), Xin-yi Chen  (陈信义), Dong-yun Li  (李冬云), Ya-yue Zhang  (张雅月), Wei Ma  (马 薇), Yu-ting Chu  (褚雨霆), Feng-qin Shi  (石凤芹), Lu Yang  (杨 璐) & Li Hou  (侯 丽)

  2. Department of Oncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, 400021, China

    Zong-lang Lai  (赖宗浪)

Authors
  1. Jing Wang  (王 婧)
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  2. Zong-lang Lai  (赖宗浪)
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  3. Xin-yi Chen  (陈信义)
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  4. Dong-yun Li  (李冬云)
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  5. Ya-yue Zhang  (张雅月)
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  6. Wei Ma  (马 薇)
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  7. Yu-ting Chu  (褚雨霆)
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  8. Feng-qin Shi  (石凤芹)
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  9. Lu Yang  (杨 璐)
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  10. Li Hou  (侯 丽)
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Corresponding author

Correspondence to Li Hou  (侯 丽).

Additional information

Supported by the Specialized Research Fund for the Doctoral Program of Ministry of Education of China (No. 20100013110008)

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Wang, J., Lai, Zl., Chen, Xy. et al. Effect of Compound Zhebei Granule (复方浙贝颗粒) combined with chemotherapy on surface markers of leukemia stem cell in patients with acute myeloid leukemia. Chin. J. Integr. Med. 22, 438–444 (2016). https://doi.org/10.1007/s11655-015-2117-2

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  • DOI: https://doi.org/10.1007/s11655-015-2117-2

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