Summary
Ethylene is a plant growth regulator that is known to influence in vitro morphogenesis. This study investigated the effects of three ethylene inhibitors, silver nitrate (AgNO3), 2,5-norbornadiene, and cobalt chloride (CoCl2), on the regeneration of cowpea from cotyledon explants. Significant increases in the percentage of regeneration occurred as a result of adding either 50 µM AgNO3 or 100 µM 2,5-norbornadiene. The number of shoots produced per explant was enhanced by adding 25 µM CoCl2 or 100 µM norbornadiene. Maximum shoot elongation was obtained with 25 µM of either CoCl2 or norbornadiene. The effect of the duration of exposure to AgNO3 was also determined. The greatest percent regeneration was obtained with the addition of 60 µM AgNO3 either to both the initiation and regeneration stages, or to only the regeneration stage. The promotive effects on organogenesis in response to ethylene inhibitors suggests an important role for ethylene in the process of in vitro morphogenesis of cowpea and may contribute to its normally low regeneration frequency.
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Brar, M.S., Moore, M.J., Al-Khayri, J.M. et al. Ethylene inhibitors promote in vitro regeneration of cowpea (Vigna unguiculata L.). In Vitro Cell.Dev.Biol.-Plant 35, 222–225 (1999). https://doi.org/10.1007/s11627-999-0082-1
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DOI: https://doi.org/10.1007/s11627-999-0082-1